{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:03:52Z","timestamp":1789661032174,"version":"4.0.1"},"reference-count":44,"publisher":"Royal Society of Chemistry (RSC)","issue":"35","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":217,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Anal. Methods"],"abstract":"<jats:p>Amino acid (AA) profiles from body fluids are clinical indicators of metabolic and hepatic diseases. This study proposes structural isomers as internal standards in tandem mass spectrometry (MS\/MS) for quantification of AAs in preventive medicine.<\/jats:p>","DOI":"10.1039\/d6ay01194b","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T16:43:18Z","timestamp":1786034598000},"page":"7718-7727","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chimeric structural isomer fragments as cost-efficient internal standards for amino acid quantification by mass spectrometry"],"prefix":"10.1039","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0546-031X","authenticated-orcid":false,"given":"Luis Alberto","family":"De Le\u00f3n-Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Advanced Genomics Unit, Cinvestav, Km. 9.6 Libramiento Norte Carr. Irapuato-Le\u00f3n, 36824 Irapuato, Gto, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6732-1958","authenticated-orcid":false,"given":"Robert","family":"Winkler","sequence":"additional","affiliation":[{"name":"Advanced Genomics Unit, Cinvestav, Km. 9.6 Libramiento Norte Carr. Irapuato-Le\u00f3n, 36824 Irapuato, Gto, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6AY01194B\/cit1\/1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1093\/nutrit\/nuaa086","volume":"78","author":"Nagao","year":"2020","journal-title":"Nutr. Rev."},{"key":"D6AY01194B\/cit2\/1","first-page":"49","volume":"12","author":"Kimura","year":"2009","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"D6AY01194B\/cit3\/1","doi-asserted-by":"crossref","first-page":"year","DOI":"10.7717\/peerj.13754","volume":"10","author":"Albores-Mendez","year":"2022","journal-title":"PeerJ"},{"key":"D6AY01194B\/cit4\/1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1097\/00000658-197911000-00003","volume":"190","author":"Freund","year":"1979","journal-title":"Ann. Surg."},{"key":"D6AY01194B\/cit5\/1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s00125-009-1637-8","volume":"53","author":"Tai","year":"2010","journal-title":"Diabetologia"},{"key":"D6AY01194B\/cit6\/1","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/nm.2307","volume":"17","author":"Wang","year":"2011","journal-title":"Nat. Med."},{"key":"D6AY01194B\/cit7\/1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s12986-018-0271-1","volume":"15","author":"Hole\u010dek","year":"2018","journal-title":"Nutr. Metab."},{"key":"D6AY01194B\/cit8\/1","doi-asserted-by":"crossref","first-page":"1579S","DOI":"10.1093\/jn\/137.6.1579S","volume":"137","author":"Dejong","year":"2007","journal-title":"J. Nutr."},{"key":"D6AY01194B\/cit9\/1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/s41572-021-00267-0","volume":"7","author":"Van Spronsen","year":"2021","journal-title":"Nature Reviews Disease Primers"},{"key":"D6AY01194B\/cit10\/1","doi-asserted-by":"crossref","first-page":"year","DOI":"10.7717\/peerj.17757","volume":"12","author":"Albores-Mendez","year":"2024","journal-title":"PeerJ"},{"key":"D6AY01194B\/cit11\/1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0021-9258(19)77791-6","volume":"192","author":"Moore","year":"1951","journal-title":"J. Biol. Chem."},{"key":"D6AY01194B\/cit12\/1","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1007\/s00726-021-03002-x","volume":"53","author":"Ga\u0142\u0118zowska","year":"2021","journal-title":"Amino Acids"},{"key":"D6AY01194B\/cit13\/1","doi-asserted-by":"crossref","first-page":"462530","DOI":"10.1016\/j.chroma.2021.462530","volume":"1655","author":"Violi","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"D6AY01194B\/cit14\/1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s10545-016-9935-z","volume":"39","author":"Prinsen","year":"2016","journal-title":"J. Inherited Metab. Dis."},{"key":"D6AY01194B\/cit15\/1","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1038\/s41592-021-01197-1","volume":"18","author":"Alseekh","year":"2021","journal-title":"Nat. Methods"},{"key":"D6AY01194B\/cit16\/1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.clinbiochem.2004.11.007","volume":"38","author":"Taylor","year":"2005","journal-title":"Clin. Biochem."},{"key":"D6AY01194B\/cit17\/1","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.1021\/jasms.4c00408","volume":"35","author":"Dubbelman","year":"2024","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"D6AY01194B\/cit18\/1","doi-asserted-by":"crossref","first-page":"MSB200861","DOI":"10.1038\/msb.2008.61","volume":"4","author":"Lange","year":"2008","journal-title":"Mol. Syst. Biol."},{"key":"D6AY01194B\/cit19\/1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nmeth.2015","volume":"9","author":"Picotti","year":"2012","journal-title":"Nat. Methods"},{"key":"D6AY01194B\/cit20\/1","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.3390\/molecules26102989","volume":"26","author":"Grocholska","year":"2021","journal-title":"Molecules"},{"key":"D6AY01194B\/cit21\/1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.jpba.2006.08.010","volume":"43","author":"Wang","year":"2007","journal-title":"J. Pharm. Biomed. Anal."},{"key":"D6AY01194B\/cit22\/1","doi-asserted-by":"crossref","first-page":"7033","DOI":"10.1021\/ac501309s","volume":"86","author":"Szarka","year":"2014","journal-title":"Anal. Chem."},{"key":"D6AY01194B\/cit23\/1","doi-asserted-by":"crossref","first-page":"11155","DOI":"10.3390\/ijms241311155","volume":"24","author":"Pallecchi","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"D6AY01194B\/cit24\/1","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1002\/jms.619","volume":"39","author":"Kushnir","year":"2004","journal-title":"J. Mass Spectrom."},{"key":"D6AY01194B\/cit25\/1","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1021\/ed100697w","volume":"87","author":"Pence","year":"2010","journal-title":"J. Chem. Educ."},{"key":"D6AY01194B\/cit26\/1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1002\/jms.1777","volume":"45","author":"Horai","year":"2010","journal-title":"J. Mass Spectrom."},{"key":"D6AY01194B\/cit27\/1","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1007\/s13361-012-0448-1","volume":"23","author":"Xiong","year":"2012","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"D6AY01194B\/cit28\/1","doi-asserted-by":"crossref","first-page":"e4613","DOI":"10.1002\/jms.4613","volume":"55","author":"Hohenester","year":"2020","journal-title":"J. Mass Spectrom."},{"key":"D6AY01194B\/cit29\/1","doi-asserted-by":"crossref","first-page":"9541","DOI":"10.1038\/s41598-018-27983-0","volume":"8","author":"Wawrzyniak","year":"2018","journal-title":"Sci. Rep."},{"key":"D6AY01194B\/cit30\/1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/978-1-4939-9639-1_16","volume-title":"Amino Acid Analysis","volume":"2030","author":"Nakayama","year":"2019","unstructured":"A.Nakayama ,  A.Imaizumi  and  H.Yoshida , in  Amino Acid Analysis , ed.  M. A. Alterman ,  Springer New York ,  New York, NY ,  2019 ,  vol. 2030 ,  pp. 207\u2013218"},{"key":"D6AY01194B\/cit31\/1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2024","unstructured":"R Core Team ,  R: A Language and Environment for Statistical Computing ,  R Foundation for Statistical Computing ,  Vienna, Austria ,  2024"},{"key":"D6AY01194B\/cit32\/1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1093\/bioinformatics\/btr645","volume":"28","author":"Gatto","year":"2012","journal-title":"Bioinformatics"},{"key":"D6AY01194B\/cit33\/1","first-page":"7","volume":"2","author":"Zeileis","year":"2002","journal-title":"R News"},{"key":"D6AY01194B\/cit34\/1","volume-title":"Bioanalytical Method Validation and Study Sample Analysis M10, ICH Technical Report","author":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use","year":"2022","unstructured":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use ,  Bioanalytical Method Validation and Study Sample Analysis M10, ICH Technical Report ,  2022"},{"key":"D6AY01194B\/cit35\/1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S1570-0232(02)00244-1","volume":"774","author":"Almeida","year":"2002","journal-title":"J. Chromatogr. B"},{"key":"D6AY01194B\/cit36\/1","doi-asserted-by":"crossref","first-page":"8959","DOI":"10.1021\/ac5018265","volume":"86","author":"Gu","year":"2014","journal-title":"Anal. Chem."},{"key":"D6AY01194B\/cit37\/1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3343\/alm.2023.43.1.5","volume":"43","author":"Cheng","year":"2023","journal-title":"Ann. Lab. Med."},{"key":"D6AY01194B\/cit38\/1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3390\/ijns11010018","volume":"11","author":"Trampu\u017e","year":"2025","journal-title":"International Journal of Neonatal Screening"},{"key":"D6AY01194B\/cit39\/1","volume-title":"Amino Acids, Quantitative, Plasma (AAQP)","author":"Mayo Clinic Laboratories","year":"2024","unstructured":"Mayo Clinic Laboratories ,  Amino Acids, Quantitative, Plasma (AAQP) ,  2024 ,  https:\/\/www.mayocliniclabs.com\/test-catalog\/overview\/9265"},{"key":"D6AY01194B\/cit40\/1","doi-asserted-by":"crossref","first-page":"e0159205","DOI":"10.1371\/journal.pone.0159205","volume":"11","author":"Kinny-K\u00f6ster","year":"2016","journal-title":"PLoS One"},{"key":"D6AY01194B\/cit41\/1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.hepres.2005.12.006","volume":"34","author":"Zhang","year":"2006","journal-title":"Hepatol. Res."},{"key":"D6AY01194B\/cit42\/1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s00216-015-9193-1","volume":"408","author":"Nanita","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"D6AY01194B\/cit43\/1","doi-asserted-by":"crossref","first-page":"3186","DOI":"10.1038\/s41467-020-17026-6","volume":"11","author":"Sarvin","year":"2020","journal-title":"Nat. Commun."},{"key":"D6AY01194B\/cit45\/1","doi-asserted-by":"crossref","first-page":"13767","DOI":"10.1021\/acs.analchem.0c02008","volume":"92","author":"Gonz\u00e1lez-Dom\u00ednguez","year":"2020","journal-title":"Anal. Chem."}],"container-title":["Analytical Methods"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/AY\/article-pdf\/doi\/10.1039\/D6AY01194B\/0\/D6AY01194B.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:16:41Z","timestamp":1789658201000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/AY\/article-lookup\/doi\/10.1039\/D6AY01194B"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":44,"journal-issue":{"issue":"35","published-print":{"date-parts":[[2026,9,17]]}},"URL":"https:\/\/doi.org\/10.1039\/d6ay01194b","relation":{"has-review":[{"id-type":"doi","id":"10.1039\/D6AY01194B\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6AY01194B\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6AY01194B\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6AY01194B\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6AY01194B\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6AY01194B\/v1\/decision1","asserted-by":"object"}]},"ISSN":["1759-9660","1759-9679"],"issn-type":[{"value":"1759-9660","type":"print"},{"value":"1759-9679","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}