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Biol."],"abstract":"<jats:p>\n                    A new (\n                    <jats:italic>R<\/jats:italic>\n                    )-selective transaminase (RTA-223, UniProt W9Z089) is identified from\n                    <jats:italic>Capronia coronata<\/jats:italic>\n                    with activity towards a range of bulky ketone substrates. We demonstrate the directed evolution of the enzyme for production of the challenging pharmaceutical target (\n                    <jats:italic>R<\/jats:italic>\n                    )-sitagliptin.\n                  <\/jats:p>","DOI":"10.1039\/d6cb00180g","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:48:10Z","timestamp":1787222890000},"page":"2053-2058","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery and evolution of an (\n                    <i>R<\/i>\n                    )-selective transaminase for production of sitagliptin"],"prefix":"10.1039","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6285-2933","authenticated-orcid":false,"given":"Matthew","family":"Treadell","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9088-4898","authenticated-orcid":false,"given":"Gareth R. E.","family":"Surman","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grayson J.","family":"Ford","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2445-126X","authenticated-orcid":false,"given":"Christopher W.","family":"Otun","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0506-8314","authenticated-orcid":false,"given":"Shiny Joseph","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Galman","sequence":"additional","affiliation":[{"name":"Johnson Matthey, 28 Cambridge science park, Cambridge, CB4 0FP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Bawn","sequence":"additional","affiliation":[{"name":"Johnson Matthey, 28 Cambridge science park, Cambridge, CB4 0FP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akash","family":"Pandya","sequence":"additional","affiliation":[{"name":"Johnson Matthey, 28 Cambridge science park, Cambridge, CB4 0FP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2147-0744","authenticated-orcid":false,"given":"Craig J.","family":"Markin","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Function, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0454-1798","authenticated-orcid":false,"given":"Anthony P.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1590-8674","authenticated-orcid":false,"given":"Jack S.","family":"Rowbotham","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, 131 Princess Street, Manchester M1 7DN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6CB00180G\/cit1\/1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/anie.202006648","volume":"60","author":"Wu","year":"2021","journal-title":"Angew. 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