{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:07:25Z","timestamp":1789726045605,"version":"4.0.1"},"reference-count":40,"publisher":"Royal Society of Chemistry (RSC)","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":252,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100018883","name":"Maurice Wilkins Centre for Molecular Biodiscovery","doi-asserted-by":"publisher","award":["MWC4657"],"award-info":[{"award-number":["MWC4657"]}],"id":[{"id":"10.13039\/501100018883","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018883","name":"Maurice Wilkins Centre for Molecular Biodiscovery","doi-asserted-by":"publisher","award":["MWC4559"],"award-info":[{"award-number":["MWC4559"]}],"id":[{"id":"10.13039\/501100018883","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001505","name":"Health Research Council of New Zealand","doi-asserted-by":"publisher","award":["22\/030"],"award-info":[{"award-number":["22\/030"]}],"id":[{"id":"10.13039\/501100001505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001505","name":"Health Research Council of New Zealand","doi-asserted-by":"publisher","award":["26\/041"],"award-info":[{"award-number":["26\/041"]}],"id":[{"id":"10.13039\/501100001505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006346","name":"Faculty of Medical and Health Sciences, University of Auckland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006346","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["RSC Chem. Biol."],"abstract":"<jats:p>Screening of COP1 E3 ligase with pooled, peptide-based combinatorial libraries and affinity selection mass spectrometry revealed new peptide binder sequences for further development as cellular probes for studying COP1 biology.<\/jats:p>","DOI":"10.1039\/d6cb00250a","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:43:06Z","timestamp":1789029786000},"update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Affinity selection mass spectrometry screening discovers new COP1 peptide binders"],"prefix":"10.1039","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1771-0001","authenticated-orcid":false,"given":"Harsha","family":"Negi","sequence":"first","affiliation":[{"name":"Department of Cancer Sciences, Faculty of Medical and Health Sciences, University of Auckland, 85 Park Rd, Grafton, Auckland, 1023, New Zealand"},{"name":"School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"},{"name":"Maurice Wikins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3103-0914","authenticated-orcid":false,"given":"Noor","family":"Radhi","sequence":"additional","affiliation":[{"name":"Department of Cancer Sciences, Faculty of Medical and Health Sciences, University of Auckland, 85 Park Rd, Grafton, Auckland, 1023, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Schiff","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0120-4737","authenticated-orcid":false,"given":"Sam A.","family":"Jamieson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Otago, 710 Cumberland St, Dunedin, 9016, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna","family":"Shea","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Hub, School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Middleditch","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Hub, School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0090-4286","authenticated-orcid":false,"given":"Robert","family":"Mullin","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0009-5869","authenticated-orcid":false,"given":"Renata","family":"Kowalczyk","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"},{"name":"Maurice Wikins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2579-4543","authenticated-orcid":false,"given":"Paul W.","family":"Harris","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Auckland, 23 Symonds St, Auckland, 1010, New Zealand"},{"name":"Maurice Wikins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2175-9537","authenticated-orcid":false,"given":"Peter D.","family":"Mace","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Otago, 710 Cumberland St, Dunedin, 9016, New Zealand"},{"name":"Maurice Wikins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3389-8377","authenticated-orcid":false,"given":"Daniel","family":"Conole","sequence":"additional","affiliation":[{"name":"Department of Cancer Sciences, Faculty of Medical and Health Sciences, University of Auckland, 85 Park Rd, Grafton, Auckland, 1023, New Zealand"},{"name":"Maurice Wikins Centre for Molecular Biodiscovery, University of Auckland, Auckland 1142, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6CB00250A\/cit1\/1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/s41580-022-00543-1","volume":"24","author":"Dikic","year":"2023","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"D6CB00250A\/cit2\/1","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/j.chembiol.2021.04.002","volume":"28","author":"Jevti\u0107","year":"2021","journal-title":"Cell Chem. Biol."},{"key":"D6CB00250A\/cit3\/1","doi-asserted-by":"crossref","first-page":"116875","DOI":"10.1016\/j.bcp.2025.116875","volume":"236","author":"Madhukar","year":"2025","journal-title":"Biochem. Pharmacol."},{"key":"D6CB00250A\/cit4\/1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1038\/nrc3271","volume":"12","author":"Marine","year":"2012","journal-title":"Nat. Rev. Cancer"},{"key":"D6CB00250A\/cit5\/1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/nature12885","volume":"506","author":"Di Stefano","year":"2014","journal-title":"Nature"},{"key":"D6CB00250A\/cit6\/1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.ccr.2006.09.012","volume":"10","author":"Keeshan","year":"2006","journal-title":"Cancer Cell"},{"key":"D6CB00250A\/cit7\/1","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/ncb2698","volume":"15","author":"Ye","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"D6CB00250A\/cit8\/1","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1126\/science.1123374","volume":"312","author":"Qi","year":"2006","journal-title":"Science"},{"key":"D6CB00250A\/cit9\/1","doi-asserted-by":"crossref","first-page":"5357","DOI":"10.1016\/j.cell.2021.09.006","volume":"184","author":"Wang","year":"2021","journal-title":"Cell"},{"key":"D6CB00250A\/cit10\/1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.str.2016.12.008","volume":"25","author":"Durzynska","year":"2017","journal-title":"Structure"},{"key":"D6CB00250A\/cit11\/1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.str.2016.03.002","volume":"24","author":"Uljon","year":"2016","journal-title":"Structure"},{"key":"D6CB00250A\/cit12\/1","first-page":"1","volume":"11","author":"Burgess","year":"2025","journal-title":"Sci. Adv."},{"key":"D6CB00250A\/cit13\/1","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1016\/j.str.2015.08.017","volume":"23","author":"Murphy","year":"2015","journal-title":"Structure"},{"key":"D6CB00250A\/cit14\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/scisignal.aau0597","volume":"11","author":"Jamieson","year":"2018","journal-title":"Sci. Signaling"},{"key":"D6CB00250A\/cit15\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15252\/embj.2019102140","volume":"38","author":"Lau","year":"2019","journal-title":"EMBO J."},{"key":"D6CB00250A\/cit16\/1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.str.2016.03.002","volume":"24","author":"Uljon","year":"2016","journal-title":"Structure"},{"key":"D6CB00250A\/cit17\/1","doi-asserted-by":"crossref","first-page":"16728","DOI":"10.1021\/acs.jmedchem.3c01431","volume":"66","author":"Sarkar","year":"2023","journal-title":"J. Med. Chem."},{"key":"D6CB00250A\/cit18\/1","doi-asserted-by":"crossref","first-page":"110184","DOI":"10.1016\/j.bioorg.2026.110184","volume":"180","author":"Liu","year":"2026","journal-title":"Bioorg. Chem."},{"key":"D6CB00250A\/cit19\/1","doi-asserted-by":"crossref","first-page":"19129","DOI":"10.1021\/jacs.3c04899","volume":"145","author":"Mata","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"D6CB00250A\/cit20\/1","first-page":"1","volume":"5","author":"Zhang","year":"2022","journal-title":"Commun. Chem."},{"key":"D6CB00250A\/cit21\/1","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1038\/s41467-020-16920-3","volume":"11","author":"Quartararo","year":"2020","journal-title":"Nat. Commun."},{"key":"D6CB00250A\/cit22\/1","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1021\/acscombsci.7b00109","volume":"19","author":"Vinogradov","year":"2017","journal-title":"ACS Comb. Sci."},{"key":"D6CB00250A\/cit23\/1","doi-asserted-by":"crossref","first-page":"19129","DOI":"10.1021\/jacs.3c04899","volume":"145","author":"Mata","year":"2023","journal-title":"J. Am. Chem. Soc."},{"key":"D6CB00250A\/cit24\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-026-71641-3","volume":"17","author":"Mata","year":"2026","journal-title":"Nat. Commun."},{"issue":"21","key":"D6CB00250A\/cit25\/1","doi-asserted-by":"crossref","first-page":"e202320045","DOI":"10.1002\/anie.202320045","volume":"63","author":"Bruce","year":"2024","journal-title":"Angew. Chem., Int. Ed."},{"key":"D6CB00250A\/cit26\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s42004-022-00737-w","volume":"5","author":"Ye","year":"2022","journal-title":"Commun. Chem."},{"key":"D6CB00250A\/cit27\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10989-022-10370-9","volume":"28","author":"Koh","year":"2022","journal-title":"Int. J. Pept. Res. Ther."},{"key":"D6CB00250A\/cit28\/1","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1038\/s41589-019-0245-2","volume":"15","author":"Touti","year":"2019","journal-title":"Nat. Chem. Biol."},{"issue":"6635","key":"D6CB00250A\/cit29\/1","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1126\/science.adf1354","volume":"379","author":"R\u00f6ssler","year":"2023","journal-title":"Science"},{"issue":"7","key":"D6CB00250A\/cit30\/1","doi-asserted-by":"crossref","first-page":"3855","DOI":"10.1021\/acs.analchem.4c04445","volume":"97","author":"Brown","year":"2025","journal-title":"Anal. Chem."},{"issue":"32","key":"D6CB00250A\/cit31\/1","doi-asserted-by":"crossref","first-page":"10817","DOI":"10.1039\/D1SC02587B","volume":"12","author":"Article","year":"2021","journal-title":"Chem Sci."},{"key":"D6CB00250A\/cit32\/1","first-page":"59","volume":"11","author":"Lee","year":"2025","journal-title":"Sci. Adv."},{"key":"D6CB00250A\/cit33\/1","doi-asserted-by":"crossref","first-page":"102501","DOI":"10.1016\/j.chempr.2025.102501","volume":"11","author":"Albin","year":"2025","journal-title":"Chem"},{"key":"D6CB00250A\/cit34\/1","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1021\/jacsau.3c00650","volume":"4","author":"Zhang","year":"2024","journal-title":"JACS Au"},{"key":"D6CB00250A\/cit35\/1","first-page":"1","volume":"5","author":"Zhang","year":"2022","journal-title":"Commun. Chem."},{"key":"D6CB00250A\/cit36\/1","first-page":"M111","volume-title":"Molecular and Cellular Proteomics 11","volume":"010587","author":"Zhang","year":"2012","unstructured":"J.Zhang ,  et al. , PEAKS DB:  De Novo  Sequencing Assisted Database Search for Sensitive and Accurate Peptide Identification.  Molecular and Cellular Proteomics 11 ,  M111 . 010587  ( 2012 )"},{"key":"D6CB00250A\/cit37\/1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nmeth1019","volume":"4","author":"Elias","year":"2007","journal-title":"Nat. Methods"},{"key":"D6CB00250A\/cit38\/1","first-page":"1","volume-title":"The Assay Guidance Manual","author":"Auld","year":"2017","unstructured":"D. S.Auld ,  et al., Assay Interference by Aggregation ,  The Assay Guidance Manual ,  2017 ,  pp. 1\u201328"},{"key":"D6CB00250A\/cit39\/1","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1021\/acs.jmedchem.4c02010","volume":"68","author":"Ackloo","year":"2025","journal-title":"J. Med. Chem."},{"key":"D6CB00250A\/cit40\/1","volume-title":"Mechanistic Design of Cell-Penetrating Disruptors for a Phospho-Dependent Interaction","author":"Bayliss","year":"2025","unstructured":"R.Bayliss ,  et al. ,  Mechanistic Design of Cell-Penetrating Disruptors for a Phospho-Dependent Interaction . Research Sqaure  https:\/\/www.researchsquare.com\/article\/rs-6862805\/v1  ( 2025 )"}],"container-title":["RSC Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/CB\/article-pdf\/doi\/10.1039\/D6CB00250A\/0\/D6CB00250A.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:32:48Z","timestamp":1789723968000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/CB\/article-lookup\/doi\/10.1039\/D6CB00250A"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":40,"URL":"https:\/\/doi.org\/10.1039\/d6cb00250a","relation":{},"ISSN":["2633-0679"],"issn-type":[{"value":"2633-0679","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}