{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T17:06:23Z","timestamp":1784048783509,"version":"3.55.0"},"reference-count":67,"publisher":"Royal Society of Chemistry (RSC)","issue":"14","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":138,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100010428","name":"Innovation and Technology Fund","doi-asserted-by":"publisher","award":["ITS\/048\/22"],"award-info":[{"award-number":["ITS\/048\/22"]}],"id":[{"id":"10.13039\/501100010428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Lab Chip"],"abstract":"<jats:p>A fully automated microfluidic SELEX that accelerates weeks-long aptamer discovery to 30 min selection rounds by integrating free-solution electrokinetic partitioning through an artificial sieve with plasmonic bead-based PCR using Au nanorods.<\/jats:p>","DOI":"10.1039\/d6lc00275g","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T09:26:27Z","timestamp":1779182787000},"page":"4117-4130","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Auto-SELEX: a fully automated microfluidic platform for rapid discovery of high-affinity aptamers"],"prefix":"10.1039","volume":"26","author":[{"given":"Yang","family":"Bu","sequence":"first","affiliation":[{"name":"Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuze","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anni","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1968-1005","authenticated-orcid":false,"given":"Yung Ching","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3151-5616","authenticated-orcid":false,"given":"Levent","family":"Yobas","sequence":"additional","affiliation":[{"name":"Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China"},{"name":"Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6LC00275G\/cit1\/1","doi-asserted-by":"crossref","first-page":"0076","DOI":"10.1038\/s41570-017-0076","volume":"1","author":"Dunn","year":"2017","journal-title":"Nat. Rev. Chem."},{"key":"D6LC00275G\/cit2\/1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.biotechadv.2018.11.001","volume":"37","author":"Wang","year":"2019","journal-title":"Biotechnol. Adv."},{"key":"D6LC00275G\/cit3\/1","doi-asserted-by":"crossref","first-page":"941","DOI":"10.3390\/molecules24050941","volume":"24","author":"Zhang","year":"2019","journal-title":"Molecules"},{"key":"D6LC00275G\/cit4\/1","doi-asserted-by":"crossref","first-page":"3175","DOI":"10.1016\/j.bios.2008.11.010","volume":"24","author":"Torres-Chavolla","year":"2009","journal-title":"Biosens. Bioelectron."},{"key":"D6LC00275G\/cit5\/1","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.3390\/ph6121507","volume":"6","author":"Shum","year":"2013","journal-title":"Pharmaceuticals"},{"key":"D6LC00275G\/cit6\/1","doi-asserted-by":"crossref","first-page":"119038","DOI":"10.1016\/j.saa.2020.119038","volume":"247","author":"Zhao","year":"2021","journal-title":"Spectrochim. Acta, Part A"},{"key":"D6LC00275G\/cit7\/1","doi-asserted-by":"crossref","first-page":"817350","DOI":"10.1155\/2013\/817350","volume":"2013","author":"Chang","year":"2013","journal-title":"J. Nucleic Acids"},{"key":"D6LC00275G\/cit8\/1","doi-asserted-by":"crossref","first-page":"434","DOI":"10.3389\/fchem.2020.00434","volume":"8","author":"McConnell","year":"2020","journal-title":"Front. Chem."},{"key":"D6LC00275G\/cit9\/1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.copbio.2016.11.020","volume":"45","author":"Nguyen","year":"2017","journal-title":"Curr. Opin. Biotechnol."},{"key":"D6LC00275G\/cit10\/1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3390\/cancers10030080","volume":"10","author":"Morita","year":"2018","journal-title":"Cancers"},{"key":"D6LC00275G\/cit11\/1","doi-asserted-by":"crossref","first-page":"830","DOI":"10.3390\/molecules23040830","volume":"23","author":"Kim","year":"2018","journal-title":"Molecules"},{"key":"D6LC00275G\/cit12\/1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.bios.2017.11.028","volume":"102","author":"Park","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"D6LC00275G\/cit13\/1","doi-asserted-by":"crossref","first-page":"5400","DOI":"10.1039\/D1AY01519B","volume":"13","author":"Mandal","year":"2021","journal-title":"Anal. Methods"},{"key":"D6LC00275G\/cit14\/1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s10753-016-0437-9","volume":"40","author":"Hu","year":"2017","journal-title":"Inflammation"},{"key":"D6LC00275G\/cit15\/1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1089\/nat.2015.0568","volume":"26","author":"Vorobyeva","year":"2016","journal-title":"Nucleic Acid Ther."},{"key":"D6LC00275G\/cit16\/1","doi-asserted-by":"crossref","first-page":"2186","DOI":"10.1021\/acs.bioconjchem.5b00291","volume":"26","author":"Zhu","year":"2015","journal-title":"Bioconjugate Chem."},{"key":"D6LC00275G\/cit17\/1","doi-asserted-by":"crossref","first-page":"3670","DOI":"10.1021\/nn301869z","volume":"6","author":"Xiao","year":"2012","journal-title":"ACS Nano"},{"key":"D6LC00275G\/cit18\/1","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.3390\/ph3061761","volume":"3","author":"Ray","year":"2010","journal-title":"Pharmaceuticals"},{"key":"D6LC00275G\/cit19\/1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"D6LC00275G\/cit20\/1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"D6LC00275G\/cit21\/1","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.3390\/ijms18102142","volume":"18","author":"Zhuo","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"D6LC00275G\/cit22\/1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00216-005-3388-9","volume":"383","author":"Stoltenburg","year":"2005","journal-title":"Anal. Bioanal. Chem."},{"key":"D6LC00275G\/cit23\/1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.2144\/02321dd04","volume":"32","author":"Bruno","year":"2002","journal-title":"BioTechniques"},{"key":"D6LC00275G\/cit24\/1","doi-asserted-by":"crossref","first-page":"4300","DOI":"10.1021\/ac0506480","volume":"77","author":"Rupcich","year":"2005","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit25\/1","doi-asserted-by":"crossref","first-page":"6107","DOI":"10.1021\/ac050836q","volume":"77","author":"Mosing","year":"2005","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit26\/1","doi-asserted-by":"crossref","first-page":"9382","DOI":"10.1021\/ja052406n","volume":"127","author":"Mendonsa","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"D6LC00275G\/cit27\/1","doi-asserted-by":"crossref","first-page":"10761","DOI":"10.1021\/ac401875h","volume":"85","author":"Jing","year":"2013","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit28\/1","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1021\/ja056943j","volume":"128","author":"Berezovski","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"D6LC00275G\/cit29\/1","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1038\/nprot.2006.200","volume":"1","author":"Berezovski","year":"2006","journal-title":"Nat. Protoc."},{"key":"D6LC00275G\/cit30\/1","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1039\/c1lc20461k","volume":"11","author":"Jing","year":"2011","journal-title":"Lab Chip"},{"key":"D6LC00275G\/cit31\/1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00216-005-0089-3","volume":"384","author":"Hybarger","year":"2006","journal-title":"Anal. Bioanal. Chem."},{"key":"D6LC00275G\/cit32\/1","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1016\/j.bios.2009.12.029","volume":"25","author":"Huang","year":"2010","journal-title":"Biosens. Bioelectron."},{"key":"D6LC00275G\/cit33\/1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.bios.2012.02.024","volume":"35","author":"Huang","year":"2012","journal-title":"Biosens. Bioelectron."},{"key":"D6LC00275G\/cit34\/1","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1039\/C4LC00187G","volume":"14","author":"Lai","year":"2014","journal-title":"Lab Chip"},{"key":"D6LC00275G\/cit35\/1","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.bios.2018.09.040","volume":"122","author":"Sinha","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"D6LC00275G\/cit36\/1","doi-asserted-by":"crossref","first-page":"26139","DOI":"10.1038\/srep26139","volume":"6","author":"Kim","year":"2016","journal-title":"Sci. Rep."},{"key":"D6LC00275G\/cit37\/1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10544-022-00643-x","volume":"25","author":"Olsen","year":"2023","journal-title":"Biomed. Microdevices"},{"key":"D6LC00275G\/cit38\/1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1021\/ac4031994","volume":"86","author":"Cao","year":"2014","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit39\/1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1021\/nn505605e","volume":"9","author":"Cao","year":"2015","journal-title":"ACS Nano"},{"key":"D6LC00275G\/cit40\/1","doi-asserted-by":"crossref","first-page":"10022","DOI":"10.1021\/acs.analchem.7b02484","volume":"89","author":"Duan","year":"2017","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit41\/1","doi-asserted-by":"crossref","first-page":"6662","DOI":"10.1039\/D5LC00785B","volume":"25","author":"Hu","year":"2025","journal-title":"Lab Chip"},{"key":"D6LC00275G\/cit42\/1","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1109\/MEMS61431.2025.10917871","volume-title":"2025 IEEE 38th International Conference on Micro Electro Mechanical Systems (MEMS)","author":"Hu","year":"2025","unstructured":"A.Hu ,  Y.Bu ,  Y.Liu ,  Y. C.Lee  and  L.Yobas , in  2025 IEEE 38th International Conference on Micro Electro Mechanical Systems (MEMS) ,  IEEE ,  2025 ,  pp. 1221\u20131224"},{"key":"D6LC00275G\/cit43\/1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.4049\/jimmunol.157.1.221","volume":"157","author":"Wiegand","year":"1996","journal-title":"J. Immunol."},{"key":"D6LC00275G\/cit44\/1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1021\/ja037832s","volume":"126","author":"Mendonsa","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"D6LC00275G\/cit45\/1","first-page":"28","volume-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol.","volume":"2","author":"Bailey","year":"1994","unstructured":"T. L.Bailey  and  C.Elkan ,  Proc. Int. Conf. Intell. Syst. Mol. Biol. ,  1994 ,  vol. 2 ,  pp. 28\u201336"},{"key":"D6LC00275G\/cit46\/1","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1093\/nar\/gkg595","volume":"31","author":"Zuker","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"D6LC00275G\/cit47\/1","doi-asserted-by":"crossref","first-page":"5387","DOI":"10.1021\/ac049857v","volume":"76","author":"Mendonsa","year":"2004","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit48\/1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1002\/elps.200600398","volume":"28","author":"Gong","year":"2007","journal-title":"Electrophoresis"},{"key":"D6LC00275G\/cit49\/1","doi-asserted-by":"crossref","first-page":"3636","DOI":"10.1021\/ac902877v","volume":"82","author":"Turgeon","year":"2010","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit50\/1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/(SICI)1098-2825(1999)13:4<145::AID-JCLA2>3.0.CO;2-C","volume":"13","author":"Iijima","year":"1999","journal-title":"J. Clin. Lab. Anal."},{"key":"D6LC00275G\/cit51\/1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0378-4347(01)00327-9","volume":"762","author":"Ren","year":"2001","journal-title":"J. Chromatogr. B: Biomed. Sci. Appl."},{"key":"D6LC00275G\/cit52\/1","doi-asserted-by":"crossref","first-page":"7652","DOI":"10.1073\/pnas.85.20.7652","volume":"85","author":"Gyllensten","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6LC00275G\/cit53\/1","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1016\/S0021-9258(18)96257-5","volume":"242","author":"Little","year":"1967","journal-title":"J. Biol. Chem."},{"key":"D6LC00275G\/cit54\/1","doi-asserted-by":"crossref","first-page":"4937","DOI":"10.1093\/nar\/17.13.4937","volume":"17","author":"Hultman","year":"1989","journal-title":"Nucleic Acids Res."},{"key":"D6LC00275G\/cit55\/1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1472-6750-7-48","volume":"7","author":"Nitsche","year":"2007","journal-title":"BMC Biotechnol."},{"key":"D6LC00275G\/cit56\/1","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1073\/pnas.0813135106","volume":"106","author":"Lou","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6LC00275G\/cit57\/1","doi-asserted-by":"crossref","first-page":"10940","DOI":"10.1021\/ac503261b","volume":"86","author":"H\u00fcnniger","year":"2014","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit58\/1","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1006\/jmbi.1998.1727","volume":"278","author":"Vant-Hull","year":"1998","journal-title":"J. Mol. Biol."},{"key":"D6LC00275G\/cit59\/1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1093\/nar\/gkaf219","volume":"53","author":"Alkhamis","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"D6LC00275G\/cit60\/1","doi-asserted-by":"crossref","first-page":"15373","DOI":"10.1073\/pnas.1009331107","volume":"107","author":"Cho","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6LC00275G\/cit61\/1","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1021\/ac302721j","volume":"85","author":"Yang","year":"2013","journal-title":"Anal. Chem."},{"key":"D6LC00275G\/cit62\/1","doi-asserted-by":"crossref","first-page":"F219","DOI":"10.1149\/1.1623767","volume":"150","author":"Thorsness","year":"2003","journal-title":"J. Electrochem. Soc."},{"key":"D6LC00275G\/cit63\/1","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1039\/b518362f","volume":"6","author":"Grover","year":"2006","journal-title":"Lab Chip"},{"key":"D6LC00275G\/cit64\/1","doi-asserted-by":"crossref","first-page":"046501","DOI":"10.1063\/1.4757968","volume":"6","author":"Liu","year":"2012","journal-title":"Biomicrofluidics"},{"key":"D6LC00275G\/cit65\/1","doi-asserted-by":"crossref","first-page":"e108","DOI":"10.1093\/nar\/gnf107","volume":"30","author":"Cox","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"D6LC00275G\/cit66\/1","doi-asserted-by":"crossref","first-page":"e183","DOI":"10.1038\/mtna.2014.34","volume":"3","author":"Ozer","year":"2014","journal-title":"Mol. Ther.--Nucleic Acids"},{"key":"D6LC00275G\/cit67\/1","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/j.biotechadv.2015.02.008","volume":"33","author":"Darmostuk","year":"2015","journal-title":"Biotechnol. Adv."}],"container-title":["Lab on a Chip"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/LC\/article-pdf\/doi\/10.1039\/D6LC00275G\/0\/D6LC00275G.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T16:13:04Z","timestamp":1784045584000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/LC\/article-lookup\/doi\/10.1039\/D6LC00275G"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":67,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2026,7,14]]}},"URL":"https:\/\/doi.org\/10.1039\/d6lc00275g","relation":{},"ISSN":["1473-0197","1473-0189"],"issn-type":[{"value":"1473-0197","type":"print"},{"value":"1473-0189","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}