{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:05:47Z","timestamp":1789571147429,"version":"build-2803163510"},"reference-count":64,"publisher":"Royal Society of Chemistry (RSC)","issue":"18","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":222,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100006469","name":"Fundo para o Desenvolvimento das Ci\u00eancias e da Tecnologia","doi-asserted-by":"publisher","award":["0021\/2022\/A1"],"award-info":[{"award-number":["0021\/2022\/A1"]}],"id":[{"id":"10.13039\/501100006469","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006469","name":"Fundo para o Desenvolvimento das Ci\u00eancias e da Tecnologia","doi-asserted-by":"publisher","award":["0002\/2024\/TFP"],"award-info":[{"award-number":["0002\/2024\/TFP"]}],"id":[{"id":"10.13039\/501100006469","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004733","name":"Universidade de Macau","doi-asserted-by":"publisher","award":["MYRG-GRG2025-00213-IAPME"],"award-info":[{"award-number":["MYRG-GRG2025-00213-IAPME"]}],"id":[{"id":"10.13039\/501100004733","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Lab Chip"],"abstract":"<jats:p>The template-free FUS-pen system enables non-contact, cross-scale manipulation of microparticles and cells. With high resolution and high cell viability (&gt;94%), it serves as an agile tool for constructing organized multicellular models.<\/jats:p>","DOI":"10.1039\/d6lc00543h","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:39:52Z","timestamp":1786437592000},"page":"4935-4950","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["FUS-pen: focused ultrasound-mediated micro-sculpting of label-free cells to create functional co-culture microenvironments"],"prefix":"10.1039","volume":"26","author":[{"given":"Yu","family":"Liu","sequence":"first","affiliation":[{"name":"Macao Centre for Research and Development in Advanced Materials, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macao S.A.R. 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1004-4923","authenticated-orcid":false,"given":"Ziyu","family":"Huang","sequence":"additional","affiliation":[{"name":"Macao Centre for Research and Development in Advanced Materials, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macao S.A.R. 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runxing","family":"Lin","sequence":"additional","affiliation":[{"name":"Macao Centre for Research and Development in Advanced Materials, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macao S.A.R. 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Macao Centre for Research and Development in Advanced Materials, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macao S.A.R. 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4180-8669","authenticated-orcid":false,"given":"Shizheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Macao Centre for Research and Development in Advanced Materials, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macao S.A.R. 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1288-6745","authenticated-orcid":false,"given":"Zhichao","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Medical Robotics, School of Biomedical Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2084-5804","authenticated-orcid":false,"given":"Yinning","family":"Zhou","sequence":"additional","affiliation":[{"name":"Macao Centre for Research and Development in Advanced Materials, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macao S.A.R. 999078, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6LC00543H\/cit1\/1","doi-asserted-by":"crossref","first-page":"103604","DOI":"10.1016\/j.semcdb.2025.103604","volume":"169","author":"Seirin-Lee","year":"2025","journal-title":"Semin. Cell Dev. Biol."},{"key":"D6LC00543H\/cit2\/1","doi-asserted-by":"crossref","first-page":"e2101321","DOI":"10.1002\/adma.202101321","volume":"34","author":"Bertassoni","year":"2022","journal-title":"Adv. Mater."},{"key":"D6LC00543H\/cit3\/1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/s41563-020-00804-4","volume":"20","author":"Garreta","year":"2021","journal-title":"Nat. Mater."},{"key":"D6LC00543H\/cit4\/1","doi-asserted-by":"crossref","first-page":"100259","DOI":"10.1016\/j.labinv.2023.100259","volume":"103","author":"De Logu","year":"2023","journal-title":"Lab. Invest."},{"key":"D6LC00543H\/cit5\/1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/s41587-024-02301-4","volume":"43","author":"Lorenzo-Mart\u00edn","year":"2025","journal-title":"Nat. Biotechnol."},{"key":"D6LC00543H\/cit6\/1","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/s41586-024-07330-2","volume":"629","author":"Lorenzo-Martin","year":"2024","journal-title":"Nature"},{"key":"D6LC00543H\/cit7\/1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1039\/D0LC01186J","volume":"21","author":"Zhang","year":"2021","journal-title":"Lab Chip"},{"key":"D6LC00543H\/cit8\/1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/s41592-019-0325-y","volume":"16","author":"Homan","year":"2019","journal-title":"Nat. Methods"},{"key":"D6LC00543H\/cit9\/1","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/nbt.2958","volume":"32","author":"Murphy","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"D6LC00543H\/cit10\/1","first-page":"1","author":"Zhang","year":"2026","journal-title":"Biomed. Mater. & Devices"},{"issue":"2","key":"D6LC00543H\/cit11\/1","doi-asserted-by":"crossref","first-page":"022003","DOI":"10.1088\/1758-5090\/ad22ee","volume":"16","author":"Zhang","year":"2024","journal-title":"Biofabrication"},{"key":"D6LC00543H\/cit12\/1","first-page":"70","volume":"9","author":"Xu","year":"2022","journal-title":"Mil. Med. Res."},{"key":"D6LC00543H\/cit13\/1","doi-asserted-by":"crossref","first-page":"106039","DOI":"10.1016\/j.isci.2023.106039","volume":"26","author":"Li","year":"2023","journal-title":"iScience"},{"issue":"106\u2013122","key":"D6LC00543H\/cit14\/1","first-page":"e126","volume":"189","author":"Wang","year":"2026","journal-title":"Cell"},{"key":"D6LC00543H\/cit15\/1","doi-asserted-by":"crossref","first-page":"e70396","DOI":"10.1002\/mco2.70396","volume":"6","author":"Dai","year":"2025","journal-title":"MedComm"},{"key":"D6LC00543H\/cit16\/1","doi-asserted-by":"crossref","first-page":"2310212","DOI":"10.1002\/adma.202310212","volume":"36","author":"Chai","year":"2024","journal-title":"Adv. Mater."},{"key":"D6LC00543H\/cit17\/1","doi-asserted-by":"crossref","first-page":"12072","DOI":"10.1021\/acsnano.2c10704","volume":"17","author":"Ren","year":"2023","journal-title":"ACS Nano"},{"key":"D6LC00543H\/cit18\/1","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1038\/s41467-024-46245-4","volume":"15","author":"Han","year":"2024","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit19\/1","doi-asserted-by":"crossref","first-page":"e2107888","DOI":"10.1002\/smll.202107888","volume":"18","author":"Noh","year":"2022","journal-title":"Small"},{"key":"D6LC00543H\/cit20\/1","doi-asserted-by":"crossref","first-page":"e08807","DOI":"10.1002\/adma.202508807","volume":"38","author":"Harder","year":"2026","journal-title":"Adv. Mater."},{"key":"D6LC00543H\/cit21\/1","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1038\/s41467-024-47631-8","volume":"15","author":"Zhang","year":"2024","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit22\/1","doi-asserted-by":"crossref","first-page":"2400161","DOI":"10.1002\/adtp.202400161","volume":"7","author":"Vighetto","year":"2024","journal-title":"Adv. Ther."},{"key":"D6LC00543H\/cit23\/1","doi-asserted-by":"crossref","first-page":"4144","DOI":"10.1038\/s41467-025-59146-x","volume":"16","author":"Zhong","year":"2025","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit24\/1","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1073\/pnas.1920099117","volume":"117","author":"Aghakhani","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6LC00543H\/cit25\/1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.bioelechem.2011.06.002","volume":"82","author":"Stacey","year":"2011","journal-title":"Bioelectrochemistry"},{"key":"D6LC00543H\/cit26\/1","doi-asserted-by":"crossref","first-page":"9401","DOI":"10.1016\/j.biomaterials.2011.08.075","volume":"32","author":"Bae","year":"2011","journal-title":"Biomaterials"},{"key":"D6LC00543H\/cit27\/1","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1140\/epjp\/s13360-022-03190-9","volume":"137","author":"Hu","year":"2022","journal-title":"Eur. Phys. J. Plus"},{"key":"D6LC00543H\/cit28\/1","doi-asserted-by":"crossref","first-page":"045501","DOI":"10.1088\/2631-7990\/adbb34","volume":"7","author":"Yin","year":"2025","journal-title":"Int. J. Extreme Manuf."},{"key":"D6LC00543H\/cit29\/1","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1038\/s41467-024-55478-2","volume":"16","author":"Luo","year":"2025","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit30\/1","doi-asserted-by":"crossref","first-page":"106441","DOI":"10.1016\/j.ultsonch.2023.106441","volume":"96","author":"Liu","year":"2023","journal-title":"Ultrason. Sonochem."},{"key":"D6LC00543H\/cit31\/1","doi-asserted-by":"crossref","first-page":"e2000462","DOI":"10.1002\/smll.202000462","volume":"16","author":"Tayebi","year":"2020","journal-title":"Small"},{"key":"D6LC00543H\/cit32\/1","doi-asserted-by":"crossref","first-page":"100357","DOI":"10.1016\/j.mtbio.2022.100357","volume":"16","author":"Di Marzio","year":"2022","journal-title":"Mater. Today Bio"},{"key":"D6LC00543H\/cit33\/1","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.3390\/mi13081362","volume":"13","author":"Leshno","year":"2022","journal-title":"Micromachines"},{"key":"D6LC00543H\/cit34\/1","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/s41467-024-55337-0","volume":"16","author":"Surappa","year":"2025","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit35\/1","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1039\/D3LC00742A","volume":"24","author":"Harley","year":"2024","journal-title":"Lab Chip"},{"key":"D6LC00543H\/cit36\/1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1038\/s41378-024-00789-z","volume":"10","author":"Yin","year":"2024","journal-title":"Microsyst. Nanoeng."},{"key":"D6LC00543H\/cit37\/1","doi-asserted-by":"crossref","first-page":"2211422","DOI":"10.1002\/adfm.202211422","volume":"33","author":"Harley","year":"2023","journal-title":"Adv. Funct. Mater."},{"key":"D6LC00543H\/cit38\/1","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/s41563-025-02169-y","volume":"24","author":"Zhao","year":"2025","journal-title":"Nat. Mater."},{"key":"D6LC00543H\/cit39\/1","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1039\/D1LC01074C","volume":"22","author":"Liu","year":"2022","journal-title":"Lab Chip"},{"key":"D6LC00543H\/cit40\/1","doi-asserted-by":"crossref","first-page":"122691","DOI":"10.1016\/j.biomaterials.2024.122691","volume":"311","author":"Li","year":"2024","journal-title":"Biomaterials"},{"key":"D6LC00543H\/cit41\/1","doi-asserted-by":"crossref","first-page":"6691","DOI":"10.1038\/s41467-024-50923-8","volume":"15","author":"Derayatifar","year":"2024","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit42\/1","doi-asserted-by":"crossref","first-page":"e1904181","DOI":"10.1002\/adma.201904181","volume":"32","author":"Ma","year":"2020","journal-title":"Adv. Mater."},{"key":"D6LC00543H\/cit43\/1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s00441-020-03397-1","volume":"386","author":"Jiang","year":"2021","journal-title":"Cell Tissue Res."},{"key":"D6LC00543H\/cit44\/1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1177\/2211068213485748","volume":"19","author":"Xie","year":"2014","journal-title":"J. Lab. Autom."},{"key":"D6LC00543H\/cit45\/1","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1039\/C9LC01124B","volume":"20","author":"Kang","year":"2020","journal-title":"Lab Chip"},{"key":"D6LC00543H\/cit46\/1","doi-asserted-by":"crossref","first-page":"8691","DOI":"10.1039\/C9SM00946A","volume":"15","author":"Collins","year":"2019","journal-title":"Soft Matter"},{"key":"D6LC00543H\/cit47\/1","doi-asserted-by":"crossref","first-page":"8686","DOI":"10.1038\/ncomms9686","volume":"6","author":"Collins","year":"2015","journal-title":"Nat. Commun."},{"key":"D6LC00543H\/cit48\/1","doi-asserted-by":"crossref","first-page":"8355","DOI":"10.1038\/s41598-021-87459-6","volume":"11","author":"Jeger-Madiot","year":"2021","journal-title":"Sci. Rep."},{"key":"D6LC00543H\/cit49\/1","doi-asserted-by":"crossref","first-page":"e2416432","DOI":"10.1002\/adma.202416432","volume":"37","author":"Ren","year":"2025","journal-title":"Adv. Mater."},{"key":"D6LC00543H\/cit50\/1","doi-asserted-by":"crossref","first-page":"136266","DOI":"10.1016\/j.matlet.2024.136266","volume":"363","author":"Zhao","year":"2024","journal-title":"Mater. Lett."},{"key":"D6LC00543H\/cit51\/1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/nprot.2016.037","volume":"11","author":"Loessner","year":"2016","journal-title":"Nat. Protoc."},{"key":"D6LC00543H\/cit52\/1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.actbio.2020.02.023","volume":"106","author":"Puckert","year":"2020","journal-title":"Acta Biomater."},{"key":"D6LC00543H\/cit53\/1","doi-asserted-by":"crossref","first-page":"111277","DOI":"10.1016\/j.eurpolymj.2022.111277","volume":"173","author":"Xiao","year":"2022","journal-title":"Eur. Polym. J."},{"key":"D6LC00543H\/cit54\/1","doi-asserted-by":"crossref","first-page":"6099","DOI":"10.1038\/s41598-017-06257-1","volume":"7","author":"Sato","year":"2017","journal-title":"Sci. Rep."},{"key":"D6LC00543H\/cit55\/1","doi-asserted-by":"crossref","first-page":"8709","DOI":"10.1021\/acs.langmuir.7b04108","volume":"34","author":"Landry","year":"2018","journal-title":"Langmuir"},{"key":"D6LC00543H\/cit56\/1","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1002\/jbm.a.37783","volume":"112","author":"McLoughlin","year":"2024","journal-title":"J. Biomed. Mater. Res., Part A"},{"key":"D6LC00543H\/cit57\/1","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1084\/jem.120.5.955","volume":"120","author":"Levine","year":"1964","journal-title":"J. Exp. Med."},{"key":"D6LC00543H\/cit58\/1","doi-asserted-by":"crossref","first-page":"102177","DOI":"10.1016\/j.xpro.2023.102177","volume":"4","author":"Guo","year":"2023","journal-title":"STAR Protoc."},{"key":"D6LC00543H\/cit59\/1","doi-asserted-by":"crossref","first-page":"eaaw9021","DOI":"10.1126\/science.aaw9021","volume":"375","author":"Gjorevski","year":"2022","journal-title":"Science"},{"key":"D6LC00543H\/cit60\/1","doi-asserted-by":"crossref","first-page":"025017","DOI":"10.1088\/1758-5090\/ac57a5","volume":"14","author":"Shao","year":"2022","journal-title":"Biofabrication"},{"key":"D6LC00543H\/cit61\/1","doi-asserted-by":"crossref","first-page":"e1802649","DOI":"10.1002\/adma.201802649","volume":"30","author":"Armstrong","year":"2018","journal-title":"Adv. Mater."},{"key":"D6LC00543H\/cit62\/1","doi-asserted-by":"crossref","first-page":"4413","DOI":"10.1039\/D3LC00448A","volume":"23","author":"Gao","year":"2023","journal-title":"Lab Chip"},{"key":"D6LC00543H\/cit63\/1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41563-020-00803-5","volume":"20","author":"Brassard","year":"2021","journal-title":"Nat. Mater."},{"key":"D6LC00543H\/cit64\/1","doi-asserted-by":"crossref","first-page":"e2005346","DOI":"10.1002\/adma.202005346","volume":"32","author":"Zhang","year":"2020","journal-title":"Adv. Mater."}],"container-title":["Lab on a Chip"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/LC\/article-pdf\/doi\/10.1039\/D6LC00543H\/0\/D6LC00543H.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:48:33Z","timestamp":1789570113000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/LC\/article-lookup\/doi\/10.1039\/D6LC00543H"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":64,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2026,9,16]]}},"URL":"https:\/\/doi.org\/10.1039\/d6lc00543h","relation":{},"ISSN":["1473-0197","1473-0189"],"issn-type":[{"value":"1473-0197","type":"print"},{"value":"1473-0189","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}