{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:50:39Z","timestamp":1792111839921,"version":"4.4.0"},"reference-count":44,"publisher":"Royal Society of Chemistry (RSC)","issue":"10","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":205,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V011324\/1"],"award-info":[{"award-number":["BB\/V011324\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/Z516880\/1"],"award-info":[{"award-number":["BB\/Z516880\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/R513246\/1"],"award-info":[{"award-number":["EP\/R513246\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["209171\/Z\/17\/Z"],"award-info":[{"award-number":["209171\/Z\/17\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Nanoscale Horiz."],"abstract":"<jats:p>Three-dimensional single-particle analysis is possible using a single AFM topology image, based on contact-point reconstruction AFM (CPR-AFM) in an integrative approach with cryo-electron microscopy maps by template matching.<\/jats:p>","DOI":"10.1039\/d6nh00151c","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T22:23:42Z","timestamp":1785018222000},"page":"2570-2581","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Three-dimensional single-particle reconstruction by atomic force microscopy imaging and template matching allows rapid structural-based validation of recombinant SARS-CoV-2 Spike protein from a single topology image"],"prefix":"10.1039","volume":"11","author":[{"given":"Liisa","family":"Lutter","sequence":"first","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Beal","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Stanley","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Roobol","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Martin","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Budge","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe E.","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Nemoto-Smith","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian R.","family":"Brown","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6028-6456","authenticated-orcid":false,"given":"Martin J.","family":"Warren","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"},{"name":"Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2762-4724","authenticated-orcid":false,"given":"C. Mark","family":"Smales","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"},{"name":"National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co. Dublin, A94 X099, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6504-0404","authenticated-orcid":false,"given":"Wei-Feng","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Kent, CT2 7NJ, Canterbury, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6NH00151C\/cit1\/1","doi-asserted-by":"publisher","first-page":"107963","DOI":"10.1016\/j.jsb.2023.107963","volume":"215","author":"Dumitru","year":"2023","journal-title":"J. Struct. Biol."},{"key":"D6NH00151C\/cit2\/1","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1038\/s41586-020-2236-6","volume":"582","author":"Pasquina-Lemonche","year":"2020","journal-title":"Nature"},{"key":"D6NH00151C\/cit3\/1","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1038\/nnano.2017.45","volume":"12","author":"Dufrene","year":"2017","journal-title":"Nat. Nanotechnol."},{"key":"D6NH00151C\/cit4\/1","doi-asserted-by":"publisher","first-page":"1234","DOI":"10.1038\/s41586-024-07559-x","volume":"637","author":"Degenhardt","year":"2025","journal-title":"Nature"},{"key":"D6NH00151C\/cit5\/1","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1042\/BST20230857","volume":"52","author":"Chitty","year":"2024","journal-title":"Biochem. Soc. Trans."},{"key":"D6NH00151C\/cit6\/1","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1515\/bmc-2020-0009","volume":"11","author":"Lutter","year":"2020","journal-title":"Biomol. Concepts"},{"key":"D6NH00151C\/cit7\/1","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/j.str.2024.11.007","volume":"33","author":"Xue","year":"2025","journal-title":"Structure"},{"key":"D6NH00151C\/cit8\/1","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1038\/s41586-021-03551-x","volume":"594","author":"Heath","year":"2021","journal-title":"Nature"},{"key":"D6NH00151C\/cit9\/1","doi-asserted-by":"publisher","first-page":"e2301766","DOI":"10.1002\/smtd.202301766","author":"Heath","year":"2024","journal-title":"Small Methods"},{"key":"D6NH00151C\/cit10\/1","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1038\/s42004-025-01741-6","volume":"8","author":"Aubrey","year":"2025","journal-title":"Commun. Chem."},{"key":"D6NH00151C\/cit11\/1","doi-asserted-by":"publisher","first-page":"167466","DOI":"10.1016\/j.jmb.2022.167466","volume":"434","author":"Lutter","year":"2022","journal-title":"J. Mol. Biol."},{"key":"D6NH00151C\/cit12\/1","doi-asserted-by":"publisher","first-page":"e1008444","DOI":"10.1371\/journal.pcbi.1008444","volume":"16","author":"Amyot","year":"2020","journal-title":"PLoS Comput. Biol."},{"key":"D6NH00151C\/cit13\/1","doi-asserted-by":"publisher","first-page":"102591","DOI":"10.1016\/j.sbi.2023.102591","volume":"80","author":"Flechsig","year":"2023","journal-title":"Curr. Opin. Struct. Biol."},{"key":"D6NH00151C\/cit14\/1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/s42004-020-00372-3","volume":"3","author":"Aubrey","year":"2020","journal-title":"Commun. Chem."},{"key":"D6NH00151C\/cit15\/1","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"},{"key":"D6NH00151C\/cit16\/1","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1056\/NEJMoa2001017","volume":"382","author":"Zhu","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"D6NH00151C\/cit17\/1","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1016\/j.cell.2020.09.018","volume":"183","author":"Yao","year":"2020","journal-title":"Cell"},{"key":"D6NH00151C\/cit18\/1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/s41580-021-00418-x","volume":"23","author":"Jackson","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"D6NH00151C\/cit19\/1","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1038\/s41586-020-2179-y","volume":"581","author":"Shang","year":"2020","journal-title":"Nature"},{"key":"D6NH00151C\/cit20\/1","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.cell.2020.02.058","volume":"181","author":"Walls","year":"2020","journal-title":"Cell"},{"key":"D6NH00151C\/cit21\/1","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1038\/s41594-021-00652-z","volume":"28","author":"Yang","year":"2021","journal-title":"Nat. Struct. Mol. Biol."},{"key":"D6NH00151C\/cit22\/1","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1038\/s41586-020-2852-1","volume":"588","author":"Barnes","year":"2020","journal-title":"Nature"},{"key":"D6NH00151C\/cit23\/1","doi-asserted-by":"publisher","first-page":"eabe5575","DOI":"10.1126\/sciadv.abe5575","volume":"7","author":"Xu","year":"2021","journal-title":"Sci. Adv."},{"key":"D6NH00151C\/cit24\/1","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1107\/S2052252520012725","volume":"7","author":"Melero","year":"2020","journal-title":"IUCrJ"},{"key":"D6NH00151C\/cit25\/1","doi-asserted-by":"publisher","first-page":"679448","DOI":"10.3389\/fbioe.2021.679448","volume":"9","author":"Budge","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"D6NH00151C\/cit26\/1","doi-asserted-by":"publisher","first-page":"101127","DOI":"10.1016\/j.jbc.2021.101127","volume":"297","author":"Olia","year":"2021","journal-title":"J. Biol. Chem."},{"key":"D6NH00151C\/cit27\/1","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1038\/s41467-019-14196-w","volume":"11","author":"Henderson","year":"2020","journal-title":"Nat. Commun."},{"key":"D6NH00151C\/cit28\/1","doi-asserted-by":"publisher","first-page":"eabe3069","DOI":"10.1126\/science.abe3069","volume":"370","author":"Ma","year":"2020","journal-title":"Science"},{"key":"D6NH00151C\/cit29\/1","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1016\/j.cell.2020.06.025","volume":"182","author":"Barnes","year":"2020","journal-title":"Cell"},{"key":"D6NH00151C\/cit30\/1","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1016\/j.jiph.2021.08.011","volume":"14","author":"Vashi","year":"2021","journal-title":"J. Infect. Public Health"},{"key":"D6NH00151C\/cit31\/1","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.addr.2021.01.001","volume":"170","author":"Pollet","year":"2021","journal-title":"Adv. Drug Delivery Rev."},{"key":"D6NH00151C\/cit32\/1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1111\/jcmm.17103","volume":"26","author":"Fertig","year":"2022","journal-title":"J. Cell. Mol. Med."},{"key":"D6NH00151C\/cit33\/1","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/s41586-020-2665-2","volume":"588","author":"Ke","year":"2020","journal-title":"Nature"},{"key":"D6NH00151C\/cit34\/1","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"D6NH00151C\/cit35\/1","doi-asserted-by":"publisher","first-page":"7926","DOI":"10.1038\/s41467-022-35641-3","volume":"13","author":"Zhu","year":"2022","journal-title":"Nat. Commun."},{"key":"D6NH00151C\/cit36\/1","doi-asserted-by":"publisher","first-page":"2675","DOI":"10.1021\/acs.nanolett.0c04465","volume":"21","author":"Kiss","year":"2021","journal-title":"Nano Lett."},{"key":"D6NH00151C\/cit37\/1","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1021\/acs.nanolett.2c04270","volume":"23","author":"Lim","year":"2023","journal-title":"Nano Lett."},{"key":"D6NH00151C\/cit38\/1","doi-asserted-by":"publisher","first-page":"12089","DOI":"10.1021\/acs.langmuir.1c01910","volume":"37","author":"O'Callahan","year":"2021","journal-title":"Langmuir"},{"key":"D6NH00151C\/cit39\/1","doi-asserted-by":"publisher","first-page":"108630","DOI":"10.1016\/j.celrep.2020.108630","volume":"34","author":"Gobeil","year":"2021","journal-title":"Cell Rep."},{"key":"D6NH00151C\/cit40\/1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1038\/s41586-020-2772-0","volume":"588","author":"Benton","year":"2020","journal-title":"Nature"},{"key":"D6NH00151C\/cit41\/1","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1038\/s41422-020-00446-w","volume":"31","author":"Wang","year":"2021","journal-title":"Cell Res."},{"key":"D6NH00151C\/cit42\/1","doi-asserted-by":"publisher","first-page":"5469","DOI":"10.1038\/s41467-021-25480-z","volume":"12","author":"Huo","year":"2021","journal-title":"Nat. Commun."},{"key":"D6NH00151C\/cit43\/1","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1126\/science.abb2507","volume":"367","author":"Wrapp","year":"2020","journal-title":"Science"},{"key":"D6NH00151C\/cit44\/1","doi-asserted-by":"publisher","first-page":"2916","DOI":"10.1214\/10-Aos799","volume":"38","author":"Botev","year":"2010","journal-title":"Ann. Stat."}],"container-title":["Nanoscale Horizons"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/NH\/article-pdf\/doi\/10.1039\/D6NH00151C\/0\/D6NH00151C.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T15:41:34Z","timestamp":1791819694000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/NH\/article-lookup\/doi\/10.1039\/D6NH00151C"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":44,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,12]]}},"URL":"https:\/\/doi.org\/10.1039\/d6nh00151c","relation":{"has-review":[{"id-type":"doi","id":"10.1039\/D6NH00151C\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6NH00151C\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6NH00151C\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6NH00151C\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6NH00151C\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6NH00151C\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1039\/D6NH00151C\/v2\/response1","asserted-by":"object"}]},"ISSN":["2055-6756","2055-6764"],"issn-type":[{"value":"2055-6756","type":"print"},{"value":"2055-6764","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}