{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:43:54Z","timestamp":1792662234483,"version":"4.5.0"},"reference-count":53,"publisher":"Royal Society of Chemistry (RSC)","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":280,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"publisher","award":["EP\/X023680\/1"],"award-info":[{"award-number":["EP\/X023680\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["Org. Biomol. Chem."],"abstract":"<jats:p>Synthesis of bacterial 'sialic acid-like' sugars, including legionaminic acid and pseudaminic acid, which are critical for virulence in numerous human pathogens, is expedited by mapping the substrate tolerance and promiscuity of wild-type aldolases.<\/jats:p>","DOI":"10.1039\/d6ob01392a","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T09:44:06Z","timestamp":1791452646000},"update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expediting chemoenzymatic access to bacterial \u2018sialic acid-like\u2019 sugars\n                    <i>via<\/i>\n                    mapping of wild type sialic acid aldolase enzyme substrate tolerance and promiscuity"],"prefix":"10.1039","author":[{"given":"Millie C.","family":"Curran","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Jeffries","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha E.","family":"Hatton","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin H.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Biology, University of York, Heslington, York, YO10 5DD, UK"},{"name":"York Biomedical Research Institute, University of York, Heslington, York, YO10 5DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0066-4419","authenticated-orcid":false,"given":"Martin A.","family":"Fascione","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK"},{"name":"York Biomedical Research Institute, University of York, Heslington, York, YO10 5DD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6OB01392A\/cit1\/1","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1039\/D4CC00077C","volume":"60","author":"Lee","year":"2024","journal-title":"Chem. Commun."},{"key":"D6OB01392A\/cit2\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2015.00649","volume":"6","author":"Merino","year":"2015","journal-title":"Front. Microbiol."},{"issue":"2","key":"D6OB01392A\/cit3\/1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.tim.2020.08.002","volume":"29","author":"McDonald","year":"2021","journal-title":"Trends Microbiol."},{"key":"D6OB01392A\/cit4\/1","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.1039\/C3RA44924F","volume":"4","author":"Zunk","year":"2014","journal-title":"RSC Adv."},{"key":"D6OB01392A\/cit5\/1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1749-6632.2012.06517.x","volume":"1253","author":"Varki","year":"2012","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"D6OB01392A\/cit6\/1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1146\/annurev-immunol-020711-075018","volume":"30","author":"Pillai","year":"2012","journal-title":"Annu. Rev. Immunol."},{"key":"D6OB01392A\/cit7\/1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0955-0674(92)90041-A","volume":"4","author":"Varki","year":"1992","journal-title":"Curr. Opin. Cell Biol."},{"issue":"16","key":"D6OB01392A\/cit8\/1","doi-asserted-by":"crossref","first-page":"2998","DOI":"10.1021\/acs.orglett.2c00894","volume":"24","author":"Siyabalapitiya Arachchige","year":"2022","journal-title":"Org. Lett."},{"key":"D6OB01392A\/cit9\/1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0008-6215(01)00235-X","volume":"335","author":"Tsvetkov","year":"2001","journal-title":"Carbohydr. Res."},{"key":"D6OB01392A\/cit10\/1","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1021\/acs.joc.5b02754","volume":"81","author":"Williams","year":"2016","journal-title":"J. Org. Chem."},{"key":"D6OB01392A\/cit11\/1","doi-asserted-by":"crossref","first-page":"13420","DOI":"10.1021\/jacs.7b06055","volume":"139","author":"Liu","year":"2017","journal-title":"J. Am. Chem. Soc."},{"key":"D6OB01392A\/cit12\/1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0008-6215(01)00041-6","volume":"331","author":"Tsvetkov","year":"2001","journal-title":"Carbohydr. Res."},{"issue":"62","key":"D6OB01392A\/cit13\/1","doi-asserted-by":"crossref","first-page":"35768","DOI":"10.1039\/C8RA07771A","volume":"8","author":"Carter","year":"2018","journal-title":"RSC Adv."},{"issue":"8","key":"D6OB01392A\/cit14\/1","doi-asserted-by":"crossref","first-page":"2848","DOI":"10.1021\/jacs.5b00455","volume":"137","author":"Matthies","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"D6OB01392A\/cit15\/1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.carres.2019.01.009","volume":"474","author":"Gintner","year":"2019","journal-title":"Carbohydr. Res."},{"key":"D6OB01392A\/cit16\/1","doi-asserted-by":"crossref","first-page":"3429","DOI":"10.1039\/D3QO00637A","volume":"10","author":"Guo","year":"2023","journal-title":"Org. Chem. Front."},{"key":"D6OB01392A\/cit17\/1","doi-asserted-by":"crossref","first-page":"6819","DOI":"10.1002\/ejoc.202000250","author":"Pradhan","year":"2020","journal-title":"Eur. J. Org. Chem."},{"key":"D6OB01392A\/cit18\/1","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1021\/cr950031q","volume":"96","author":"Gijsen","year":"1996","journal-title":"Chem. Rev."},{"issue":"4","key":"D6OB01392A\/cit19\/1","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1021\/cb500067z","volume":"9","author":"Daniels","year":"2014","journal-title":"ACS Chem. Biol."},{"key":"D6OB01392A\/cit20\/1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molcatb.2012.05.016","volume":"83","author":"Groher","year":"2012","journal-title":"J. Mol. Catal. B: Enzym."},{"issue":"2","key":"D6OB01392A\/cit21\/1","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1006\/jmbi.1996.0769","volume":"266","author":"Lawrence","year":"1997","journal-title":"J. Mol. Biol."},{"issue":"26","key":"D6OB01392A\/cit22\/1","doi-asserted-by":"crossref","first-page":"10138","DOI":"10.1021\/ja00052a008","volume":"114","author":"Lin","year":"1992","journal-title":"J. Am. Chem. Soc."},{"key":"D6OB01392A\/cit23\/1","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1002\/anie.200604799","volume":"46","author":"Huang","year":"2007","journal-title":"Angew. Chem., Int. Ed."},{"key":"D6OB01392A\/cit24\/1","doi-asserted-by":"crossref","first-page":"3663","DOI":"10.1021\/jo00117a016","volume":"60","author":"Fitz","year":"1995","journal-title":"J. Org. Chem."},{"issue":"110","key":"D6OB01392A\/cit25\/1","doi-asserted-by":"crossref","first-page":"6481","DOI":"10.1021\/ja00227a031","author":"Kim","year":"1988","journal-title":"J. Am. Chem. Soc."},{"key":"D6OB01392A\/cit26\/1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.enzmictec.2016.07.003","volume":"92","author":"Chen","year":"2016","journal-title":"Enzyme Microb. Technol."},{"issue":"13","key":"D6OB01392A\/cit27\/1","doi-asserted-by":"crossref","first-page":"e202200074","DOI":"10.1002\/cbic.202200074","volume":"23","author":"Hu","year":"2022","journal-title":"ChemBioChem"},{"key":"D6OB01392A\/cit28\/1","doi-asserted-by":"crossref","first-page":"7485","DOI":"10.1039\/D4OB00874J","volume":"22","author":"Hu","year":"2024","journal-title":"Org. Biomol. Chem."},{"issue":"29","key":"D6OB01392A\/cit29\/1","doi-asserted-by":"crossref","first-page":"e202403133","DOI":"10.1002\/anie.202403133","volume":"63","author":"Mishra","year":"2024","journal-title":"Angew. Chem., Int. Ed."},{"key":"D6OB01392A\/cit30\/1","doi-asserted-by":"crossref","first-page":"2929","DOI":"10.1002\/anie.201712022","volume":"57","author":"Santra","year":"2018","journal-title":"Angew. Chem., Int. Ed."},{"key":"D6OB01392A\/cit31\/1","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1039\/C9OB02476J","volume":"18","author":"McArthur","year":"2020","journal-title":"Org. Biomol. Chem."},{"issue":"16","key":"D6OB01392A\/cit32\/1","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.3390\/molecules29163980","volume":"29","author":"Kooner","year":"2024","journal-title":"Molecules"},{"issue":"1","key":"D6OB01392A\/cit33\/1","doi-asserted-by":"crossref","first-page":"e19750","DOI":"10.1002\/ange.202519750","volume":"138","author":"Yang","year":"2025","journal-title":"Angew. Chem."},{"key":"D6OB01392A\/cit34\/1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1007\/s00253-008-1506-2","volume":"79","author":"Li","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"D6OB01392A\/cit35\/1","doi-asserted-by":"crossref","first-page":"9341","DOI":"10.1038\/srep09341","volume":"5","author":"Ji","year":"2015","journal-title":"Sci. Rep."},{"key":"D6OB01392A\/cit36\/1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1574-6968.2012.02663.x","volume":"336","author":"Gruteser","year":"2012","journal-title":"Microbiol. Lett."},{"key":"D6OB01392A\/cit37\/1","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/1471-2148-9-118","volume":"9","author":"Almagro-Moreno","year":"2009","journal-title":"BMC Evol. Biol."},{"issue":"7","key":"D6OB01392A\/cit38\/1","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1128\/AEM.02927-10","volume":"77","author":"S\u00e1nchez-Carr\u00f3n","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"D6OB01392A\/cit39\/1","doi-asserted-by":"crossref","first-page":"7450","DOI":"10.1021\/jf3014102","volume":"60","author":"Garcia-Garcia","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"D6OB01392A\/cit40\/1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.enzmictec.2003.10.020","volume":"35","author":"Lee","year":"2004","journal-title":"Enzyme Microb. Technol."},{"issue":"5","key":"D6OB01392A\/cit41\/1","doi-asserted-by":"crossref","first-page":"e96976","DOI":"10.1371\/journal.pone.0096976","volume":"9","author":"Garcia-Garcia","year":"2014","journal-title":"PLoS One"},{"key":"D6OB01392A\/cit42\/1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/S0008-6215(97)10106-9","volume":"306","author":"Maru","year":"1998","journal-title":"Carbohydr. Res."},{"issue":"3","key":"D6OB01392A\/cit43\/1","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/S1389-1723(02)80026-3","volume":"93","author":"Maru","year":"2002","journal-title":"J. Biosci. Bioeng."},{"key":"D6OB01392A\/cit44\/1","doi-asserted-by":"crossref","first-page":"11583","DOI":"10.1039\/D1RA01070K","volume":"11","author":"Niedzwiecka","year":"2021","journal-title":"RSC Adv."},{"key":"D6OB01392A\/cit45\/1","doi-asserted-by":"crossref","first-page":"24811","DOI":"10.1002\/anie.202107181","volume":"60","author":"Meng","year":"2021","journal-title":"Angew. Chem., Int. Ed."},{"issue":"42","key":"D6OB01392A\/cit46\/1","doi-asserted-by":"crossref","first-page":"28630","DOI":"10.1021\/jacs.4c10835","volume":"146","author":"Kumawat","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"D6OB01392A\/cit47\/1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1006\/jmbi.2000.4138","volume":"303","author":"Barbosa","year":"2000","journal-title":"J. Mol. Biol."},{"issue":"47","key":"D6OB01392A\/cit48\/1","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1021\/bi4011754","volume":"52","author":"Huynh","year":"2013","journal-title":"Biochemistry"},{"key":"D6OB01392A\/cit49\/1","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1002\/cbic.202000019","volume":"21","author":"Flack","year":"2020","journal-title":"ChemBioChem"},{"key":"D6OB01392A\/cit50\/1","doi-asserted-by":"crossref","first-page":"15008","DOI":"10.1021\/jacs.8b09654","volume":"140","author":"Dhakal","year":"2018","journal-title":"J. Am. Chem. Soc."},{"key":"D6OB01392A\/cit51\/1","doi-asserted-by":"crossref","first-page":"100699","DOI":"10.1016\/j.jbc.2021.100699","volume":"296","author":"Kentache","year":"2021","journal-title":"J. Biol. Chem."},{"key":"D6OB01392A\/cit52\/1","doi-asserted-by":"crossref","first-page":"12018","DOI":"10.1002\/anie.201606006","volume":"55","author":"Hassan","year":"2016","journal-title":"Angew. Chem., Int. Ed."},{"issue":"10","key":"D6OB01392A\/cit53\/1","doi-asserted-by":"crossref","first-page":"3272","DOI":"10.1021\/bi702364s","volume":"47","author":"Glaze","year":"2008","journal-title":"Biochem."}],"container-title":["Organic &amp; Biomolecular Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/OB\/article-pdf\/doi\/10.1039\/D6OB01392A\/0\/D6OB01392A.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:33:30Z","timestamp":1792661610000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/OB\/article-lookup\/doi\/10.1039\/D6OB01392A"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":53,"URL":"https:\/\/doi.org\/10.1039\/d6ob01392a","relation":{"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv.15007810\/v1","asserted-by":"object"}]},"ISSN":["1477-0520","1477-0539"],"issn-type":[{"value":"1477-0520","type":"print"},{"value":"1477-0539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}