{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T21:50:17Z","timestamp":1782856217169,"version":"3.54.5"},"reference-count":70,"publisher":"Royal Society of Chemistry (RSC)","issue":"23","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":110,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/PR36327\/ADV\/90\/280\/2020 dated 27\/09\/2021"],"award-info":[{"award-number":["BT\/PR36327\/ADV\/90\/280\/2020 dated 27\/09\/2021"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["RSC Adv."],"abstract":"<jats:p>\n                    The environmental persistence of\n                    <jats:italic>Bacillus anthracis<\/jats:italic>\n                    spores continues to challenge rapid detection and surveillance of anthrax, necessitating simple, field-deployable analytical tools.\n                  <\/jats:p>","DOI":"10.1039\/d6ra02624a","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T12:31:46Z","timestamp":1776774706000},"page":"20733-20746","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synthetic peptide-based latex agglutination test for the detection of\n                    <i>Bacillus anthracis<\/i>\n                    spores from soil samples"],"prefix":"10.1039","volume":"16","author":[{"given":"Maria Anto Dani","family":"Nishanth","sequence":"first","affiliation":[{"name":"ICAR-National Meat Research Institute, Chengicherla, Boduppal P.O., Hyderabad-500 092, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4276-1129","authenticated-orcid":false,"given":"Jess","family":"Vergis","sequence":"additional","affiliation":[{"name":"College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi P.O.-673 576, Wayanad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinay","family":"Joshi","sequence":"additional","affiliation":[{"name":"Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh","family":"Jindal","sequence":"additional","affiliation":[{"name":"Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivaraj","family":"Murag","sequence":"additional","affiliation":[{"name":"Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Veterinary Biologicals, Karnataka Veterinary, Animal and Fisheries Sciences University, Hebbal-560 024, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Rani","family":"Prameela","sequence":"additional","affiliation":[{"name":"State Level Diagnostic Laboratory, Sri Venkateswara Veterinary University, Tirupati-517 502, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niveditha","family":"Pollumahanti","sequence":"additional","affiliation":[{"name":"ICAR-National Meat Research Institute, Chengicherla, Boduppal P.O., Hyderabad-500 092, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satya Veer Singh","family":"Malik","sequence":"additional","affiliation":[{"name":"College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi P.O.-673 576, Wayanad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhadeo Baliram","family":"Barbuddhe","sequence":"additional","affiliation":[{"name":"ICAR-National Meat Research Institute, Chengicherla, Boduppal P.O., Hyderabad-500 092, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-4708","authenticated-orcid":false,"given":"Deepak Bhiwa","family":"Rawool","sequence":"additional","affiliation":[{"name":"ICAR-National Meat Research Institute, Chengicherla, Boduppal P.O., Hyderabad-500 092, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6RA02624A\/cit1\/1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-3-031-86002-7_3","volume-title":"Harnessing Biotechnology Tools for Product Development","author":"Nwinyi","year":"2025","unstructured":"O. C.Nwinyi ,  A. T.Ajayi  and  A. T.Owolabi ,  Advancements in Bacillus anthracis Diagnostic and Treatment Techniques , in  Harnessing Biotechnology Tools for Product Development ,  2025 ,  pp. 29\u201342"},{"key":"D6RA02624A\/cit2\/1","first-page":"2018","volume-title":"Research Square","author":"Aqel","year":"2025","unstructured":"A. A.Aqel ,  M.Abulubad ,  H.Alzoubi ,  D. S.Schabacker ,  S.Forrester ,  S.Schlueter ,  M.Khemmani ,  A. J.Wolfe ,  T.Yaqub ,  M.Alsbou  and  Y.Gaber ,  Detection, molecular epidemiology, and mapping of Anthrax outbreaks in Jordan , in  Research Square ,  2025 ,  pp. 2018\u20132021"},{"key":"D6RA02624A\/cit3\/1","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1002\/ddr.21803","volume":"82","author":"Leonard","year":"2021","journal-title":"Drug Dev. Res."},{"key":"D6RA02624A\/cit4\/1","first-page":"24","volume":"3","author":"Popescu","year":"2020","journal-title":"Rom. Mil. Think."},{"key":"D6RA02624A\/cit5\/1","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0140-6736(05)76173-0","volume":"353","author":"Morris","year":"1999","journal-title":"Lancet"},{"key":"D6RA02624A\/cit6\/1","doi-asserted-by":"crossref","first-page":"106778","DOI":"10.1016\/j.mimet.2023.106778","volume":"211","author":"Nishanth","year":"2023","journal-title":"J. Microbiol. Methods"},{"key":"D6RA02624A\/cit7\/1","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1038\/s41564-019-0435-4","volume":"4","author":"Carlson","year":"2019","journal-title":"Nat. Microbiol."},{"key":"D6RA02624A\/cit8\/1","first-page":"711","volume":"37","author":"Bylaiah","year":"2022","journal-title":"Int. J. Spec. Educ."},{"key":"D6RA02624A\/cit9\/1","first-page":"81","volume":"10","author":"Olani","year":"2020","journal-title":"Eur. J. Biol. Res."},{"key":"D6RA02624A\/cit10\/1","doi-asserted-by":"crossref","first-page":"0303413","DOI":"10.1371\/journal.pone.0303413","volume":"19","author":"Arede","year":"2024","journal-title":"PLoS One"},{"key":"D6RA02624A\/cit11\/1","volume-title":"Anthrax","author":"CDC","unstructured":"CDC ,  Anthrax , available online: accessed on 10 October 2019"},{"key":"D6RA02624A\/cit12\/1","first-page":"215","volume":"7","author":"Meshram","year":"2021","journal-title":"IP Int. J. Med. Microbiol. Trop. Dis."},{"key":"D6RA02624A\/cit13\/1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1080\/00450618.2019.1629020","volume":"53","author":"Bowman","year":"2021","journal-title":"Aust. J. Forensic Sci."},{"key":"D6RA02624A\/cit14\/1","doi-asserted-by":"crossref","first-page":"6269","DOI":"10.1039\/C5AN01304F","volume":"140","author":"Hutchison","year":"2015","journal-title":"Analyst"},{"key":"D6RA02624A\/cit15\/1","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.tifs.2022.03.023","volume":"123","author":"Meng","year":"2022","journal-title":"Trends Food Sci. Technol."},{"key":"D6RA02624A\/cit16\/1","doi-asserted-by":"crossref","first-page":"4319","DOI":"10.18203\/2320-6012.ijrms20163286","volume":"4","author":"Parwanto","year":"2016","journal-title":"Int. Res. J. Med. Sci."},{"key":"D6RA02624A\/cit17\/1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/jms.3747","volume":"51","author":"Duriez","year":"2016","journal-title":"J. Mass Spectrom."},{"key":"D6RA02624A\/cit18\/1","volume-title":"Humans and Animals","author":"Anthrax","year":"2008","unstructured":"W. H. O.Anthrax . in  Humans and Animals ,  World Health Organization ,  Geneva, Switzerland ,  2008"},{"key":"D6RA02624A\/cit19\/1","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1080\/10826068.2018.1525564","volume":"48","author":"Budama-Kilinc","year":"2018","journal-title":"Prep. Biochem. Biotechnol."},{"key":"D6RA02624A\/cit20\/1","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.17582\/journal.aavs\/2021\/9.9.1292.1301","volume":"9","author":"Roushdy","year":"2021","journal-title":"Adv. Anim. Vet. Sci."},{"key":"D6RA02624A\/cit21\/1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1128\/jcm.27.2.309-312.1989","volume":"27","author":"Inzana","year":"1989","journal-title":"J. Clin. Microbiol."},{"key":"D6RA02624A\/cit22\/1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.21307\/pjm-2018-030","volume":"67","author":"Rastawicki","year":"2018","journal-title":"Pol. J. Microbiol."},{"key":"D6RA02624A\/cit23\/1","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1128\/CVI.00342-06","volume":"14","author":"Hajra","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"D6RA02624A\/cit24\/1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.vetpar.2012.04.010","volume":"189","author":"Moraveji","year":"2012","journal-title":"Vet. Parasitol."},{"key":"D6RA02624A\/cit25\/1","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1111\/lam.12204","volume":"58","author":"Sumithra","year":"2014","journal-title":"Lett. Appl. Microbiol."},{"key":"D6RA02624A\/cit26\/1","first-page":"78","volume":"1","author":"Sumitha","year":"2014","journal-title":"Int. Res. J. Biol. Sci."},{"key":"D6RA02624A\/cit27\/1","first-page":"206","volume":"7","author":"Felix","year":"2019","journal-title":"Poult. Fish. Wildl. Sci."},{"key":"D6RA02624A\/cit28\/1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.mimet.2006.09.014","volume":"68","author":"Turnbull","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"D6RA02624A\/cit29\/1","first-page":"713","author":"David","year":"2010","journal-title":"Clin. Ophthalmol."},{"key":"D6RA02624A\/cit30\/1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3389\/fcimb.2017.00270","volume":"7","author":"Liang","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"D6RA02624A\/cit31\/1","doi-asserted-by":"crossref","first-page":"0228270","DOI":"10.1371\/journal.pone.0228270","volume":"15","author":"Norris","year":"2020","journal-title":"PLoS One"},{"key":"D6RA02624A\/cit32\/1","doi-asserted-by":"crossref","first-page":"623","DOI":"10.3201\/eid0906.020377","volume":"9","author":"Whitney","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"D6RA02624A\/cit33\/1","doi-asserted-by":"crossref","first-page":"31370","DOI":"10.1038\/srep31370","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"key":"D6RA02624A\/cit34\/1","doi-asserted-by":"crossref","first-page":"116","DOI":"10.4103\/ijmm.IJMM_19_111","volume":"37","author":"Kumar","year":"2019","journal-title":"Indian J. Med. Microbiol."},{"key":"D6RA02624A\/cit35\/1","doi-asserted-by":"crossref","first-page":"105859","DOI":"10.1016\/j.mimet.2020.105859","volume":"170","author":"Yadav","year":"2020","journal-title":"J. Microbiol. Methods"},{"key":"D6RA02624A\/cit36\/1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/macp.1992.021930119","volume":"193","author":"Charleux","year":"1992","journal-title":"Makromol. Chem."},{"key":"D6RA02624A\/cit37\/1","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1182\/blood.V74.8.2698.2698","volume":"74","author":"Bale","year":"1989","journal-title":"Blood"},{"key":"D6RA02624A\/cit38\/1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2136\/sssabookser5.2.c3","volume-title":"Methods of Soil Analysis: Part 2 Microbiological and Biochemical Properties","volume":"59","author":"Wolf","year":"1994","unstructured":"D. C.Wolf  and  H. D.Skipper ,  Soil sterilization , in  Methods of Soil Analysis: Part 2 Microbiological and Biochemical Properties ,  1994 , vol.  59 ,  pp. 41\u201351"},{"key":"D6RA02624A\/cit39\/1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/1471-2180-13-167","volume":"13","author":"Fasanella","year":"2013","journal-title":"BMC Microbiol."},{"key":"D6RA02624A\/cit40\/1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1046\/j.1472-765x.2001.00966.x","volume":"33","author":"Dragon","year":"2001","journal-title":"Lett. Appl. Microbiol."},{"key":"D6RA02624A\/cit41\/1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1472-765X.2008.02375.x","volume":"47","author":"Marston","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"D6RA02624A\/cit42\/1","first-page":"138","author":"Bradley","year":"2011","journal-title":"Remed. J."},{"key":"D6RA02624A\/cit43\/1","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.4315\/0362-028X.JFP-12-048","volume":"75","author":"Shields","year":"2012","journal-title":"J. Food Prot."},{"key":"D6RA02624A\/cit44\/1","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1046\/j.1365-2672.2003.02038.x","volume":"95","author":"Cheun","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"D6RA02624A\/cit45\/1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.5455\/javar.2022.i599","volume":"9","author":"Alam","year":"2022","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"D6RA02624A\/cit46\/1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2672.2006.03111.x","volume":"102","author":"Carrera","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"D6RA02624A\/cit47\/1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s00203-007-0295-9","volume":"189","author":"Baweja","year":"2008","journal-title":"Arch. Microbiol."},{"key":"D6RA02624A\/cit48\/1","doi-asserted-by":"crossref","first-page":"S129","DOI":"10.1016\/j.ijfoodmicro.2010.07.036","volume":"145","author":"Fricker","year":"2011","journal-title":"Int.\nJ. Food Microbiol."},{"key":"D6RA02624A\/cit49\/1","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1061\/(ASCE)0733-9372(2005)131:9(1245)","volume":"131","author":"Blatchley III","year":"2005","journal-title":"J. Environ. Eng."},{"key":"D6RA02624A\/cit50\/1","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1111\/j.1751-1097.2008.00387.x","volume":"84","author":"Coohill","year":"2008","journal-title":"Photochem. Photobiol."},{"key":"D6RA02624A\/cit51\/1","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1128\/AEM.69.2.1327-1330.2003","volume":"69","author":"Nicholson","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"D6RA02624A\/cit52\/1","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1128\/aem.62.7.2221-2227.1996","volume":"62","author":"Xue","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"D6RA02624A\/cit53\/1","doi-asserted-by":"crossref","first-page":"6841","DOI":"10.1128\/AEM.69.11.6841-6847.2003","volume":"69","author":"Knurr","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"D6RA02624A\/cit54\/1","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1021\/ja0656649","volume":"129","author":"Acharya","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA02624A\/cit55\/1","volume-title":"Development of Novel Fluorescence-Based Methods for Detection of Bacillus Anthracis Spores","author":"Schumacher","year":"2008","unstructured":"W. C.Schumacher ,  Development of Novel Fluorescence-Based Methods for Detection of Bacillus Anthracis Spores ,  Doctoral dissertation, The Ohio State University ,  2008"},{"key":"D6RA02624A\/cit56\/1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.vetimm.2009.12.011","volume":"135","author":"da Silva","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"D6RA02624A\/cit57\/1","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1163\/156856200743931","volume":"11","author":"Davalos-Pantoja","year":"2000","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"D6RA02624A\/cit58\/1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1590\/S0103-84782014000100025","volume":"44","author":"Munhoz","year":"2013","journal-title":"Cienc. Rural"},{"key":"D6RA02624A\/cit59\/1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.21055\/0370-1069-2011-4(110)-71-75","volume":"4","author":"Khlyntseva","year":"2011","journal-title":"Probl. Osobo Opasnykh Infekts."},{"key":"D6RA02624A\/cit60\/1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.diagmicrobio.2009.02.001","volume":"64","author":"AuCoin","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"D6RA02624A\/cit61\/1","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1007\/s13337-013-0162-z","volume":"24","author":"Joshi","year":"2013","journal-title":"Indian J. Virol."},{"key":"D6RA02624A\/cit62\/1","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1093\/infdis\/160.4.706","volume":"160","author":"Harrison","year":"1989","journal-title":"J. Infect. Dis."},{"key":"D6RA02624A\/cit63\/1","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1128\/CVI.00023-06","volume":"13","author":"Mabry","year":"2006","journal-title":"Clin. Vaccine Immunol."},{"key":"D6RA02624A\/cit64\/1","doi-asserted-by":"crossref","first-page":"5175","DOI":"10.1128\/IAI.00719-07","volume":"75","author":"Moayeri","year":"2007","journal-title":"Infect. Immun."},{"key":"D6RA02624A\/cit65\/1","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1007\/s13213-014-0932-x","volume":"65","author":"Silvestri","year":"2015","journal-title":"Ann. Microbiol."},{"key":"D6RA02624A\/cit66\/1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3390\/bios6040061","volume":"6","author":"Waller","year":"2016","journal-title":"Biosensors"},{"key":"D6RA02624A\/cit67\/1","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1128\/aem.62.9.3474-3476.1996","volume":"62","author":"Bruno","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"D6RA02624A\/cit68\/1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/jam.12206","volume":"115","author":"Thomas","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"D6RA02624A\/cit69\/1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1007\/s12088-012-0334-3","volume":"53","author":"Ghosh","year":"2013","journal-title":"Indian J. Microbiol."},{"key":"D6RA02624A\/cit70\/1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s12010-013-0145-y","volume":"170","author":"Patel","year":"2013","journal-title":"Appl. Biochem. Biotechnol."}],"container-title":["RSC Advances"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/en\/content\/articlepdf\/2026\/RA\/D6RA02624A","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T20:40:47Z","timestamp":1782852047000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.rsc.org\/ra\/article\/16\/23\/20733-20746\/1244270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":70,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2026,4,20]]}},"URL":"https:\/\/doi.org\/10.1039\/d6ra02624a","relation":{},"ISSN":["2046-2069"],"issn-type":[{"value":"2046-2069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}