{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:33:31Z","timestamp":1793277211077,"version":"4.5.0"},"reference-count":70,"publisher":"Royal Society of Chemistry (RSC)","issue":"47","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":286,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM149220"],"award-info":[{"award-number":["R35GM149220"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["RSC Adv."],"abstract":"<jats:p>\n                    Backbone modification in the\n                    <jats:italic>de novo<\/jats:italic>\n                    metalloenzyme MID1-zinc manipulates inherent chain flexibility in a dynamic helix adjacent to the active site, impacting thermal folded stability and catalytic function.\n                  <\/jats:p>","DOI":"10.1039\/d6ra05101d","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:19:29Z","timestamp":1791976769000},"page":"62876-62883","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulating thermal folded stability and catalytic activity of a\n                    <i>de novo<\/i>\n                    metalloenzyme through chemical backbone modification"],"prefix":"10.1039","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9335-6070","authenticated-orcid":false,"given":"Jacob A.","family":"Wolfe","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2927-1739","authenticated-orcid":false,"given":"W. Seth","family":"Horne","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6RA05101D\/cit1\/1","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1038\/nature06522","volume":"450","author":"Henzler-Wildman","year":"2007","journal-title":"Nature"},{"key":"D6RA05101D\/cit2\/1","doi-asserted-by":"crossref","first-page":"4704","DOI":"10.1002\/cctc.202000665","volume":"12","author":"Agarwal","year":"2020","journal-title":"ChemCatChem"},{"key":"D6RA05101D\/cit3\/1","doi-asserted-by":"crossref","first-page":"4402","DOI":"10.1021\/bi2002289","volume":"50","author":"Bar-Even","year":"2011","journal-title":"Biochemistry"},{"key":"D6RA05101D\/cit4\/1","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1021\/acs.biochem.5b00621","volume":"54","author":"Bar-Even","year":"2015","journal-title":"Biochemistry"},{"key":"D6RA05101D\/cit5\/1","doi-asserted-by":"crossref","first-page":"11324","DOI":"10.1021\/jacs.0c04924","volume":"142","author":"Crean","year":"2020","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit6\/1","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1038\/s41467-018-03562-9","volume":"9","author":"Otten","year":"2018","journal-title":"Nat. Commun."},{"key":"D6RA05101D\/cit7\/1","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1126\/science.abd3623","volume":"370","author":"Otten","year":"2020","journal-title":"Science"},{"key":"D6RA05101D\/cit8\/1","doi-asserted-by":"crossref","first-page":"eabn7738","DOI":"10.1126\/sciadv.abn7738","volume":"8","author":"Yabukarski","year":"2022","journal-title":"Sci. Adv."},{"key":"D6RA05101D\/cit9\/1","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1002\/pro.3379","volume":"27","author":"Boehr","year":"2018","journal-title":"Protein Sci."},{"key":"D6RA05101D\/cit10\/1","doi-asserted-by":"crossref","first-page":"11532","DOI":"10.1021\/acscatal.1c01776","volume":"11","author":"Hindson","year":"2021","journal-title":"ACS Catal."},{"key":"D6RA05101D\/cit11\/1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/s41557-020-0420-9","volume":"12","author":"Horne","year":"2020","journal-title":"Nat. Chem."},{"key":"D6RA05101D\/cit12\/1","doi-asserted-by":"crossref","first-page":"17045","DOI":"10.1073\/pnas.0407820101","volume":"101","author":"Valiyaveetil","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6RA05101D\/cit13\/1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/nchembio766","volume":"2","author":"Bang","year":"2006","journal-title":"Nat. Chem. Biol."},{"key":"D6RA05101D\/cit14\/1","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1073\/pnas.0711908105","volume":"105","author":"Liu","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6RA05101D\/cit15\/1","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1039\/C4SC01094A","volume":"5","author":"Reinert","year":"2014","journal-title":"Chem. Sci."},{"key":"D6RA05101D\/cit16\/1","doi-asserted-by":"crossref","first-page":"11798","DOI":"10.1039\/D2SC04427G","volume":"13","author":"Santhouse","year":"2022","journal-title":"Chem. Sci."},{"key":"D6RA05101D\/cit17\/1","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1039\/D3SC03976E","volume":"15","author":"Santhouse","year":"2024","journal-title":"Chem. Sci."},{"key":"D6RA05101D\/cit18\/1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1126\/science.1566069","volume":"256","author":"Schnolzer","year":"1992","journal-title":"Science"},{"key":"D6RA05101D\/cit19\/1","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1021\/ja952790w","volume":"118","author":"Liu","year":"1996","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit20\/1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1021\/acs.bioconjchem.9b00110","volume":"30","author":"Kofoed","year":"2019","journal-title":"Bioconjugate Chem."},{"key":"D6RA05101D\/cit21\/1","doi-asserted-by":"crossref","first-page":"8522","DOI":"10.1021\/ja026114n","volume":"124","author":"Arnold","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit22\/1","doi-asserted-by":"crossref","first-page":"12670","DOI":"10.1021\/ja075865s","volume":"129","author":"Tam","year":"2007","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit23\/1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1002\/pro.2208","volume":"22","author":"Arnold","year":"2013","journal-title":"Protein Sci."},{"key":"D6RA05101D\/cit24\/1","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.1002\/anie.201400945","volume":"53","author":"Mayer","year":"2014","journal-title":"Angew. Chem., Int. Ed."},{"key":"D6RA05101D\/cit25\/1","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1002\/cbic.201800200","volume":"19","author":"Caserta","year":"2018","journal-title":"ChemBioChem"},{"key":"D6RA05101D\/cit26\/1","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1021\/acs.accounts.9b00032","volume":"52","author":"Koebke","year":"2019","journal-title":"Acc. Chem. Res."},{"key":"D6RA05101D\/cit27\/1","doi-asserted-by":"crossref","first-page":"6679","DOI":"10.1021\/acs.inorgchem.2c00901","volume":"61","author":"Ghosh","year":"2022","journal-title":"Inorg. Chem."},{"key":"D6RA05101D\/cit28\/1","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1038\/s41467-023-37941-8","volume":"14","author":"Chino","year":"2023","journal-title":"Nat. Commun."},{"key":"D6RA05101D\/cit29\/1","doi-asserted-by":"crossref","first-page":"102509","DOI":"10.1016\/j.cbpa.2024.102509","volume":"81","author":"Wolfe","year":"2024","journal-title":"Curr. Opin. Chem. Biol."},{"key":"D6RA05101D\/cit30\/1","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1126\/science.1604320","volume":"256","author":"Milton","year":"1992","journal-title":"Science"},{"key":"D6RA05101D\/cit31\/1","doi-asserted-by":"crossref","first-page":"11075","DOI":"10.1021\/ja00150a006","volume":"117","author":"Fitzgerald","year":"1995","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit32\/1","doi-asserted-by":"crossref","first-page":"2997","DOI":"10.1039\/C4SC03877K","volume":"6","author":"Vinogradov","year":"2015","journal-title":"Chem. Sci."},{"key":"D6RA05101D\/cit33\/1","doi-asserted-by":"crossref","first-page":"3997","DOI":"10.1093\/nar\/gkx079","volume":"45","author":"Pech","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"D6RA05101D\/cit34\/1","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.chempr.2019.01.001","volume":"5","author":"Wang","year":"2019","journal-title":"Chem"},{"key":"D6RA05101D\/cit35\/1","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1038\/s41587-021-00969-6","volume":"39","author":"Fan","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"D6RA05101D\/cit36\/1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/s41557-023-01411-x","volume":"16","author":"Zhang","year":"2024","journal-title":"Nat. Chem."},{"key":"D6RA05101D\/cit37\/1","doi-asserted-by":"crossref","first-page":"4188","DOI":"10.1021\/jacs.4c13832","volume":"147","author":"Fittolani","year":"2025","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit38\/1","doi-asserted-by":"crossref","first-page":"7151","DOI":"10.1021\/ja970826+","volume":"119","author":"Chapman","year":"1997","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit39\/1","doi-asserted-by":"crossref","first-page":"8105","DOI":"10.1021\/ja0006090","volume":"122","author":"Nakhle","year":"2000","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit40\/1","doi-asserted-by":"crossref","first-page":"9503","DOI":"10.1016\/S0040-4020(00)00835-8","volume":"56","author":"Baca","year":"2000","journal-title":"Tetrahedron"},{"key":"D6RA05101D\/cit41\/1","doi-asserted-by":"crossref","first-page":"6620","DOI":"10.1021\/bi020700b","volume":"42","author":"Silinski","year":"2003","journal-title":"Biochemistry"},{"key":"D6RA05101D\/cit42\/1","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1021\/cb200300w","volume":"6","author":"Lee","year":"2011","journal-title":"ACS Chem. Biol."},{"key":"D6RA05101D\/cit43\/1","doi-asserted-by":"crossref","first-page":"20982","DOI":"10.1073\/pnas.1111202108","volume":"108","author":"Torbeev","year":"2011","journal-title":"Proc. Natl. Acad. Sci.\nU. S. A."},{"key":"D6RA05101D\/cit44\/1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0022-2836(02)01036-7","volume":"324","author":"Bartlett","year":"2002","journal-title":"J. Mol. Biol."},{"key":"D6RA05101D\/cit45\/1","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/nchem.1201","volume":"4","author":"Zastrow","year":"2012","journal-title":"Nat. Chem."},{"key":"D6RA05101D\/cit46\/1","doi-asserted-by":"crossref","first-page":"3933","DOI":"10.1021\/bi201881p","volume":"51","author":"Der","year":"2012","journal-title":"Biochemistry"},{"key":"D6RA05101D\/cit47\/1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1002\/pro.2622","volume":"24","author":"Raymond","year":"2015","journal-title":"Protein Sci."},{"key":"D6RA05101D\/cit48\/1","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/s41467-017-00541-4","volume":"8","author":"Watkins","year":"2017","journal-title":"Nat. Commun."},{"key":"D6RA05101D\/cit49\/1","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1126\/science.aau3744","volume":"362","author":"Studer","year":"2018","journal-title":"Science"},{"key":"D6RA05101D\/cit50\/1","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1073\/pnas.1915054117","volume":"117","author":"Stenner","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6RA05101D\/cit51\/1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/s41586-022-05278-9","volume":"610","author":"Bhattacharya","year":"2022","journal-title":"Nature"},{"key":"D6RA05101D\/cit52\/1","first-page":"1","author":"Schnettler","year":"2024","journal-title":"Nat. Chem."},{"key":"D6RA05101D\/cit53\/1","doi-asserted-by":"crossref","first-page":"e202304367","DOI":"10.1002\/chem.202304367","volume":"30","author":"Borghesani","year":"2024","journal-title":"Chem. \u2013 Eur. J."},{"key":"D6RA05101D\/cit54\/1","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/s41467-017-00541-4","volume":"8","author":"Watkins","year":"2017","journal-title":"Nat. Commun."},{"key":"D6RA05101D\/cit55\/1","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1111\/evo.13275","volume":"71","author":"Miller","year":"2017","journal-title":"Evolution"},{"key":"D6RA05101D\/cit56\/1","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1126\/science.adt7268","volume":"388","author":"Hou","year":"2025","journal-title":"Science"},{"key":"D6RA05101D\/cit57\/1","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1021\/ja208015j","volume":"134","author":"Der","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit58\/1","doi-asserted-by":"crossref","first-page":"100919","DOI":"10.1016\/j.xpro.2021.100919","volume":"2","author":"Kawale","year":"2021","journal-title":"STAR Protoc."},{"key":"D6RA05101D\/cit59\/1","doi-asserted-by":"crossref","first-page":"103073","DOI":"10.1016\/j.sbi.2025.103073","volume":"93","author":"Brennan","year":"2025","journal-title":"Curr. Opin. Struct. Biol."},{"key":"D6RA05101D\/cit60\/1","doi-asserted-by":"crossref","first-page":"6747","DOI":"10.1021\/bi00481a001","volume":"29","author":"Karle","year":"1990","journal-title":"Biochemistry"},{"key":"D6RA05101D\/cit61\/1","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1126\/science.2237415","volume":"250","author":"O'Neil","year":"1990","journal-title":"Science"},{"key":"D6RA05101D\/cit62\/1","doi-asserted-by":"crossref","first-page":"13292","DOI":"10.1021\/jacs.7b07930","volume":"139","author":"Fisher","year":"2017","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit63\/1","doi-asserted-by":"crossref","first-page":"9151","DOI":"10.1073\/pnas.0801135105","volume":"105","author":"Horne","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6RA05101D\/cit64\/1","doi-asserted-by":"crossref","first-page":"9396","DOI":"10.1021\/jacs.8b05162","volume":"140","author":"Fisher","year":"2018","journal-title":"J. Am. Chem. Soc."},{"key":"D6RA05101D\/cit65\/1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nature03798","volume":"436","author":"Eathiraj","year":"2005","journal-title":"Nature"},{"key":"D6RA05101D\/cit66\/1","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1021\/acs.biochem.5c00687","volume":"65","author":"Page","year":"2026","journal-title":"Biochemistry"},{"key":"D6RA05101D\/cit67\/1","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.bpj.2018.01.002","volume":"114","author":"Fleming","year":"2018","journal-title":"Biophys. J."},{"key":"D6RA05101D\/cit68\/1","first-page":"E8153","volume":"115","author":"Jenkins","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6RA05101D\/cit69\/1","doi-asserted-by":"crossref","first-page":"7891","DOI":"10.1074\/jbc.M212508200","volume":"278","author":"D'Amico","year":"2003","journal-title":"J. Biol. Chem."},{"key":"D6RA05101D\/cit70\/1","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1038\/nature06407","volume":"450","author":"Henzler-Wildman","year":"2007","journal-title":"Nature"}],"container-title":["RSC Advances"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/RA\/article-pdf\/doi\/10.1039\/D6RA05101D\/0\/D6RA05101D.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T11:52:24Z","timestamp":1793274744000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/RA\/article-lookup\/doi\/10.1039\/D6RA05101D"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":70,"journal-issue":{"issue":"47","published-print":{"date-parts":[[2026,10,29]]}},"URL":"https:\/\/doi.org\/10.1039\/d6ra05101d","relation":{},"ISSN":["2046-2069"],"issn-type":[{"value":"2046-2069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}