{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:00:53Z","timestamp":1791392453339,"version":"4.3.3"},"reference-count":55,"publisher":"Royal Society of Chemistry (RSC)","issue":"44","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":263,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","award":["Fundamental Fund: fiscal year 2026 (R016941010)"],"award-info":[{"award-number":["Fundamental Fund: fiscal year 2026 (R016941010)"]}],"id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","award":["N42A680269"],"award-info":[{"award-number":["N42A680269"]}],"id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["rsc.org"],"crossmark-restriction":true},"short-container-title":["RSC Adv."],"abstract":"<jats:p>\n                    Leuconostoc mesenteroides\n                    <jats:sc>l<\/jats:sc>\n                    -asparaginase (\n                    <jats:italic>Lm<\/jats:italic>\n                    ASNase) exhibits high specificity for\n                    <jats:sc>l<\/jats:sc>\n                    -asparagine with no detectable\n                    <jats:sc>l<\/jats:sc>\n                    -glutaminase activity. MD simulations support selective\n                    <jats:sc>l<\/jats:sc>\n                    -asparagine binding, and\n                    <jats:italic>Lm<\/jats:italic>\n                    ASNase exhibits anticancer activity.\n                  <\/jats:p>","DOI":"10.1039\/d6ra05175h","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:19:54Z","timestamp":1790003994000},"page":"56129-56140","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel glutaminase-free\n                    <scp>l<\/scp>\n                    -asparaginase from\n                    <i>Leuconostoc mesenteroides<\/i>\n                    BN1: biochemical properties, molecular dynamics, and anticancer activity"],"prefix":"10.1039","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9322-0261","authenticated-orcid":false,"given":"Thanapon","family":"Charoenwongpaiboon","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karan","family":"Wangpaiboon","sequence":"additional","affiliation":[{"name":"Center of Excellence in Structural and Computational Biology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanisa","family":"Srichompoo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praphasri","family":"Septham","sequence":"additional","affiliation":[{"name":"Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phatchanat","family":"Klaihmon","sequence":"additional","affiliation":[{"name":"Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujittra","family":"Khampang","sequence":"additional","affiliation":[{"name":"Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surapol","family":"Issaragrisil","sequence":"additional","affiliation":[{"name":"Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Bangkok Hematology Center, Wattanosoth Hospital, BDMS Center of Excellence for Cancer, Bangkok, 10310, Thailand"},{"name":"Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2206-7065","authenticated-orcid":false,"given":"Chanchao","family":"Lorthongpanich","sequence":"additional","affiliation":[{"name":"Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"},{"name":"Mechanobiology and Stem Cell Fate Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"292","published-online":{"date-parts":[[2026]]},"reference":[{"key":"D6RA05175H\/cit1\/1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.52547\/ibj.3494","volume":"26","author":"Shahnazari","year":"2022","journal-title":"Iran. Biomed. J."},{"key":"D6RA05175H\/cit2\/1","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1016\/j.bbrc.2008.10.118","volume":"377","author":"Cappelletti","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"D6RA05175H\/cit3\/1","first-page":"1173","volume":"81","author":"Kebeish","year":"2016","journal-title":"Biochemistry"},{"key":"D6RA05175H\/cit4\/1","doi-asserted-by":"crossref","first-page":"17664","DOI":"10.1074\/jbc.M116.728485","volume":"291","author":"Nguyen","year":"2016","journal-title":"J. Biol. Chem."},{"key":"D6RA05175H\/cit5\/1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s12088-019-00806-0","volume":"59","author":"Aishwarya","year":"2019","journal-title":"Indian J. Microbiol."},{"key":"D6RA05175H\/cit6\/1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s13568-024-01700-9","volume":"14","author":"Abdelrazek","year":"2024","journal-title":"AMB Express"},{"key":"D6RA05175H\/cit7\/1","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1158\/0008-5472.CAN-17-2106","volume":"78","author":"Nguyen","year":"2018","journal-title":"Cancer Res."},{"key":"D6RA05175H\/cit8\/1","first-page":"720","volume":"42","author":"Krishnapura","year":"2016","journal-title":"Crit. Rev. Microbiol."},{"key":"D6RA05175H\/cit9\/1","doi-asserted-by":"crossref","first-page":"e7246","DOI":"10.1002\/cam4.7246","volume":"13","author":"Ishida","year":"2024","journal-title":"Cancer Med."},{"key":"D6RA05175H\/cit10\/1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/hon.2207","volume":"34","author":"Yong","year":"2016","journal-title":"Hematol. Oncol."},{"key":"D6RA05175H\/cit11\/1","doi-asserted-by":"crossref","first-page":"217404","DOI":"10.1016\/j.canlet.2024.217404","volume":"611","author":"Van Trimpont","year":"2025","journal-title":"Cancer Lett."},{"key":"D6RA05175H\/cit12\/1","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1038\/s41556-019-0415-1","volume":"21","author":"Pathria","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"D6RA05175H\/cit13\/1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ccell.2017.12.003","volume":"33","author":"Gwinn","year":"2018","journal-title":"Cancer Cell"},{"key":"D6RA05175H\/cit14\/1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/1097-0142(197002)25:2<253::AID-CNCR2820250204>3.0.CO;2-U","volume":"25","author":"Oettgen","year":"1970","journal-title":"Cancer"},{"key":"D6RA05175H\/cit15\/1","doi-asserted-by":"crossref","first-page":"127998","DOI":"10.1016\/j.ijbiomac.2023.127998","volume":"254","author":"de Lima","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"D6RA05175H\/cit16\/1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ejca.2021.11.016","volume":"162","author":"Brigitha","year":"2022","journal-title":"Eur. J. Cancer"},{"key":"D6RA05175H\/cit17\/1","doi-asserted-by":"crossref","first-page":"e000858","DOI":"10.1136\/esmoopen-2020-000858","volume":"5","author":"Burke","year":"2020","journal-title":"ESMO Open"},{"key":"D6RA05175H\/cit18\/1","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.2217\/fon-2021-1288","volume":"18","author":"Burke","year":"2022","journal-title":"Future Oncol."},{"key":"D6RA05175H\/cit19\/1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1080\/17425255.2023.2233412","volume":"19","author":"Alqahtani","year":"2023","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"D6RA05175H\/cit20\/1","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1073\/pnas.90.4.1474","volume":"90","author":"Swain","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"D6RA05175H\/cit21\/1","doi-asserted-by":"crossref","first-page":"9428","DOI":"10.1021\/jf402636w","volume":"61","author":"Jia","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"D6RA05175H\/cit22\/1","doi-asserted-by":"crossref","first-page":"3756","DOI":"10.1038\/s41598-019-40512-x","volume":"9","author":"Alrumman","year":"2019","journal-title":"Sci. Rep."},{"key":"D6RA05175H\/cit23\/1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1021\/acs.jafc.0c06609","volume":"69","author":"Ran","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"D6RA05175H\/cit24\/1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0014-5793(93)80943-O","volume":"328","author":"Miller","year":"1993","journal-title":"FEBS Lett."},{"key":"D6RA05175H\/cit25\/1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1080\/10826068.2021.1931881","volume":"52","author":"da Cunha","year":"2022","journal-title":"Prep. Biochem. Biotechnol."},{"key":"D6RA05175H\/cit26\/1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1385\/ABAB:135:1:33","volume":"135","author":"Mishra","year":"2006","journal-title":"Appl. Biochem. Biotechnol."},{"key":"D6RA05175H\/cit27\/1","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1016\/j.ijbiomac.2018.09.015","volume":"120","author":"Aghaeepoor","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"D6RA05175H\/cit28\/1","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1049\/iet-nbt.2017.0156","volume":"12","author":"Varshosaz","year":"2018","journal-title":"IET Nanobiotechnol."},{"key":"D6RA05175H\/cit29\/1","doi-asserted-by":"crossref","first-page":"96","DOI":"10.3390\/foods13010096","volume":"13","author":"Ro\u015fca","year":"2024","journal-title":"Foods"},{"key":"D6RA05175H\/cit30\/1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1080\/00380768.1995.10419587","volume":"41","author":"Kanazawa","year":"1995","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"D6RA05175H\/cit31\/1","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1385\/1-59259-890-0","volume-title":"The Proteomics Protocols Handbook","volume":"571","author":"Gasteiger","year":"2005","unstructured":"E.Gasteiger ,  C.Hoogland ,  A.Gattiker ,  S. E.Duvaud ,  M. R.Wilkins ,  R. D.Appel  and  A.Bairoch , in  The Proteomics Protocols Handbook , ed.  J. M. Walker ,  Humana Press ,  Totowa, NJ ,  2005 , vol.  571 ,  pp. 571\u2013607 ,  10.1385\/1-59259-890-0"},{"key":"D6RA05175H\/cit32\/1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/pro.3290","volume":"27","author":"Sievers","year":"2018","journal-title":"Protein Sci."},{"key":"D6RA05175H\/cit33\/1","doi-asserted-by":"crossref","first-page":"W465","DOI":"10.1093\/nar\/gkn180","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"D6RA05175H\/cit34\/1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"D6RA05175H\/cit35\/1","doi-asserted-by":"crossref","first-page":"W368","DOI":"10.1093\/nar\/gki464","volume":"33","author":"Gordon","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"D6RA05175H\/cit36\/1","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1021\/acs.jcim.1c00203","volume":"61","author":"Eberhardt","year":"2021","journal-title":"J. Chem. Inf. Model."},{"key":"D6RA05175H\/cit37\/1","doi-asserted-by":"crossref","first-page":"31523","DOI":"10.1021\/acs.jafc.5c11841","volume":"73","author":"Charoenwongpaiboon","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"D6RA05175H\/cit38\/1","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1021\/ct400341p","volume":"9","author":"Roe","year":"2013","journal-title":"J. Chem. Theory Comput."},{"key":"D6RA05175H\/cit39\/1","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1021\/ct300418h","volume":"8","author":"Miller III","year":"2012","journal-title":"J. Chem. Theory Comput."},{"key":"D6RA05175H\/cit40\/1","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"D6RA05175H\/cit41\/1","doi-asserted-by":"crossref","first-page":"4183","DOI":"10.1111\/febs.16042","volume":"288","author":"Lubkowski","year":"2021","journal-title":"FEBS J."},{"key":"D6RA05175H\/cit42\/1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.3389\/fmicb.2014.00486","volume":"5","author":"Upadhyay","year":"2014","journal-title":"Front. Microbiol."},{"key":"D6RA05175H\/cit43\/1","doi-asserted-by":"crossref","first-page":"100076","DOI":"10.1016\/j.microb.2024.100076","volume":"3","author":"Rath","year":"2024","journal-title":"Microbe"},{"key":"D6RA05175H\/cit44\/1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1146\/annurev-physiol-021119-034627","volume":"82","author":"Boedtkjer","year":"2020","journal-title":"Annu. Rev. Physiol."},{"key":"D6RA05175H\/cit45\/1","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s11274-012-1213-0","volume":"29","author":"Mohan Kumar","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"D6RA05175H\/cit46\/1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/s40360-018-0242-1","volume":"19","author":"El-Naggar","year":"2018","journal-title":"BMC Pharmacol. Toxicol."},{"key":"D6RA05175H\/cit47\/1","doi-asserted-by":"crossref","first-page":"e0148877","DOI":"10.1371\/journal.pone.0148877","volume":"11","author":"Husain","year":"2016","journal-title":"PLoS One"},{"key":"D6RA05175H\/cit48\/1","doi-asserted-by":"crossref","first-page":"3260","DOI":"10.3390\/polym16233260","volume":"16","author":"Alharthi","year":"2024","journal-title":"Polymers"},{"key":"D6RA05175H\/cit49\/1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/s12866-025-03833-8","volume":"25","author":"Saleh","year":"2025","journal-title":"BMC Microbiol."},{"key":"D6RA05175H\/cit50\/1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.ijbiomac.2019.10.258","volume":"143","author":"Farahat","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"D6RA05175H\/cit51\/1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.2478\/s11756-018-0054-1","volume":"73","author":"Chohan","year":"2018","journal-title":"Biologia"},{"key":"D6RA05175H\/cit52\/1","doi-asserted-by":"crossref","first-page":"e0336433","DOI":"10.1371\/journal.pone.0336433","volume":"20","author":"Rodr\u00edguez-Vargas","year":"2025","journal-title":"PLoS One"},{"key":"D6RA05175H\/cit53\/1","doi-asserted-by":"crossref","first-page":"135458","DOI":"10.1016\/j.ijbiomac.2024.135458","volume":"279","author":"Abdullah","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"D6RA05175H\/cit54\/1","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1007\/s13205-015-0300-y","volume":"5","author":"Kishore","year":"2015","journal-title":"3 Biotech"},{"key":"D6RA05175H\/cit55\/1","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1096\/fj.11-191254","volume":"26","author":"Bansal","year":"2012","journal-title":"Faseb. J."}],"container-title":["RSC Advances"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/pubs.rsc.org\/RA\/article-pdf\/doi\/10.1039\/D6RA05175H\/0\/D6RA05175H.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:45:24Z","timestamp":1791391524000},"score":1,"resource":{"primary":{"URL":"http:\/\/pubs.rsc.org\/RA\/article-lookup\/doi\/10.1039\/D6RA05175H"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":55,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2026,10,7]]}},"URL":"https:\/\/doi.org\/10.1039\/d6ra05175h","relation":{},"ISSN":["2046-2069"],"issn-type":[{"value":"2046-2069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}