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The metabolic impact of heterologous protein production in Escherichia colicells is of particular interest, since there are numerous cellular stresses triggered during this process that limit the overall productivity, e.g. the stringent response. Because the knowledge on the metabolic responses in recombinant bioprocesses is still scarce, metabolic footprinting can provide relevant information on the intrinsic metabolic adjustments. Thus, the metabolic footprints generated by E. coli W3110 and the \u0394relA mutant strain during recombinant fed-batch fermentations at different experimental conditions were measured and interpreted. The IPTG-induction of the heterologous protein expression resulted in the rapid accumulation of inhibitors of the glyoxylate shunt in the culture broth, suggesting the clearance of this anaplerotic route to replenish the TCA intermediaries withdrawn for the additional formation of the heterologous protein. Nutritional shifts were also critical in the recombinant cellular metabolism, indicating that cells employ diverse strategies to counteract imbalances in the cellular metabolism, including the secretion of certain metabolites that are, most likely, used as a metabolic relief to survival processes.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c0mb00143k","type":"journal-article","created":{"date-parts":[[2010,12,9]],"date-time":"2010-12-09T04:30:39Z","timestamp":1291869039000},"page":"899-910","source":"Crossref","is-referenced-by-count":32,"title":["Metabolic footprint analysis of recombinant\n                    <i>Escherichia coli<\/i>\n                    strains during fed-batch fermentations"],"prefix":"10.1093","volume":"7","author":[{"given":"S\u00f3nia","family":"Carneiro","sequence":"first","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar a , 4710-057 Braga, Portugal irocha@deb.uminho.pt +351 253 678 986 +351 253 604 408"}]},{"given":"Silas G","family":"Villas-B\u00f4as","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Auckland b , Private Bag 92019, Auckland 1142 New Zealand s.villas-boas@auckland.ac.nz +64 9 3737416 +64 9 3737599"}]},{"given":"Eug\u00e9nio C","family":"Ferreira","sequence":"additional","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar a , 4710-057 Braga, Portugal irocha@deb.uminho.pt +351 253 678 986 +351 253 604 408"}]},{"given":"Isabel","family":"Rocha","sequence":"additional","affiliation":[{"name":"IBB\u2014Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar a , 4710-057 Braga, Portugal irocha@deb.uminho.pt +351 253 678 986 +351 253 604 408"}]}],"member":"286","published-online":{"date-parts":[[2011,4,20]]},"reference":[{"issue":"1","key":"2026012913140152700_cit1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00449-007-0163-7","volume":"31","author":"Gnoth","year":"2008","journal-title":"Bioprocess Biosyst. 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