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Their effects on cellular metabolism were monitored in a human triple-negative metastatic breast cancer cell line (MDA-MB-231) by Raman and synchrotron-radiation infrared microspectroscopies, for different drug concentrations (2\u20138 \u03bcM) at 48 h exposure. Multivariate data analysis was applied (unsupervised PCA), unveiling drug- and concentration-dependent effects: apart from discrimination between control and drug-treated cells, a clear separation was obtained for the different agents studied \u2013 mononuclear <italic>vs.<\/italic> polynuclear, and Pt(<sc>ii<\/sc>) <italic>vs.<\/italic> Pd(<sc>ii<\/sc>). Spectral biomarkers of drug action were identified, as well as the cellular response to the chemotherapeutic insult. The main effect of the tested compounds was found to be on DNA, lipids and proteins, the Pd(<sc>ii<\/sc>) agent having a more significant impact on proteins while its Pt(<sc>ii<\/sc>) homologue affected the cellular lipid content at lower concentrations, which suggests the occurrence of distinct and unconventional pathways of cytotoxicity for these dinuclear polyamine complexes. Raman and FTIR microspectroscopies were confirmed as powerful non-invasive techniques to obtain unique spectral signatures of the biochemical impact and physiological reaction of cells to anticancer agents.<\/p>","DOI":"10.1039\/c5fd00148j","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T09:34:11Z","timestamp":1452504851000},"page":"273-298","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Chemotherapeutic response to cisplatin-like drugs in human breast cancer cells probed by vibrational microspectroscopy"],"prefix":"10.1039","volume":"187","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1280-3321","authenticated-orcid":false,"given":"A. L. M.","family":"Batista de Carvalho","sequence":"first","affiliation":[{"name":"\u201cQu\u00edmica-F\u00edsica Molecular\u201d"},{"name":"Univ. 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