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In this first article, the development of a comprehensive kinetic dynamic model is described. The model is based on in vivo NMR data that consist of concentration trends in key glycolytic metabolites and cofactors. The model structure and parameter values are identified with a customized optimization strategy that uses as its core the method of dynamic flux estimation. For the first time, a dynamic model with a single parameter set fits all available glycolytic time course data under anaerobic operation. The model captures observations that had not been addressed so far and suggests the existence of regulatory effects that had been observed in other species, but not in L. lactis. The companion paper uses this model to analyze details of the dynamic control of glycolysis under aerobic and anaerobic conditions.<\/jats:p>\n                  <jats:p\/>","DOI":"10.1039\/c5mb00331h","type":"journal-article","created":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T04:02:28Z","timestamp":1446782548000},"page":"23-36","update-policy":"https:\/\/doi.org\/10.1039\/rsc_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["New insights into the complex regulation of the glycolytic pathway in\n                    <i>Lactococcus lactis<\/i>\n                    . I. Construction and diagnosis of a comprehensive dynamic model"],"prefix":"10.1093","volume":"12","author":[{"given":"Sepideh","family":"Dolatshahi","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Georgia Institute of Technology a , Atlanta, GA, 30332, USA \u00a0 sepideh@gatech.edu \u00a0 llfonseca@gatech.edu \u00a0 eberhard.voit@bme.gatech.edu"}]},{"given":"Luis L","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Georgia Institute of Technology a , Atlanta, GA, 30332, USA \u00a0 sepideh@gatech.edu \u00a0 llfonseca@gatech.edu \u00a0 eberhard.voit@bme.gatech.edu"}]},{"given":"Eberhard O","family":"Voit","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Georgia Institute of Technology a , Atlanta, GA, 30332, USA \u00a0 sepideh@gatech.edu \u00a0 llfonseca@gatech.edu \u00a0 eberhard.voit@bme.gatech.edu"}]}],"member":"286","published-online":{"date-parts":[[2015,11,6]]},"reference":[{"key":"2026010815192113600_cit1","author":"Dolatshahi","year":"2015","journal-title":"Mol. 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