{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:21:35Z","timestamp":1773422495426,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":1409,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in <jats:italic>Amoeba proteus<\/jats:italic> [Nishihara, Shimmen and Sonobe (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#b23\">2004<\/jats:ext-link>) Cell Struct. Funct. <jats:bold>29<\/jats:bold>, 85\u201390].<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. In the present study, we cloned an AQP gene from <jats:italic>A. proteus<\/jats:italic> [ApAQP (<jats:italic>A. proteus<\/jats:italic> AQP)] that encodes a 295\u2010amino\u2010acid protein. The protein has six putative TMs (transmembrane domains) and two NPA (Asn\u2010Pro\u2010Ala) motifs, which are conserved among various AQPs and are thought to be involved in the formation of water channels that span the lipid bilayer. Using <jats:italic>Xenopus<\/jats:italic> oocytes, we have demonstrated that the ApAQP protein product can function as a water channel. Immunofluorescence microscopy with anti\u2010ApAQP antibody revealed that ApAQP is detected on the CV membrane and on the vesicles around the CV. The presence of V\u2010ATPase (vacuolar H<jats:sup>+<\/jats:sup>\u2010ATPase) on the vesicle membrane around the CV was also detected.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. Our data on ApAQP allow us to provide the first informed explanation of the high water permeability of the CV membrane in amoeba. Moreover, the results suggest that vesicles possessing V\u2010ATPase are involved in generating an osmotic gradient. Based on our findings, we propose a new hypothesis for the mechanism of CV function.<\/jats:p>","DOI":"10.1042\/bc20070091","type":"journal-article","created":{"date-parts":[[2008,2,19]],"date-time":"2008-02-19T10:25:44Z","timestamp":1203416744000},"page":"179-188","source":"Crossref","is-referenced-by-count":32,"title":["Presence of aquaporin and V\u2010ATPase on the contractile vacuole of <i>Amoeba proteus<\/i>"],"prefix":"10.1111","volume":"100","author":[{"given":"Eri","family":"Nishihara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etsuo","family":"Yokota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Tazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidefumi","family":"Orii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maki","family":"Katsuhara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kensuke","family":"Kataoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisako","family":"Igarashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Moriyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruo","family":"Shimmen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Sonobe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200510-109JH"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-1878(200011)22:11<1035::AID-BIES10>3.0.CO;2-A"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20040095"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1242\/jcs.115.14.2893","article-title":"Dynamics of the vacuolar H+\u2010ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells","volume":"115","author":"Clarke M.","year":"2002","journal-title":"J. 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