{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T21:59:29Z","timestamp":1762898369732},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":1134,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. Ribonucleases have been well studied in yeast and bacteria, but their biological significance to developmental processes in multicellular organisms is not well understood. However, there is increasing evidence that specific timed transcript degradation is critical for regulation of many cellular processes, including translational repression, nonsense\u2010mediated decay and RNA interference. The <jats:italic>Drosophila<\/jats:italic> gene <jats:italic>pacman<\/jats:italic> is highly homologous to the major yeast exoribonuclease <jats:italic>XRN1<\/jats:italic> and is the only known cytoplasmic 5\u2032\u20133\u2032 exoribonuclease in eukaryotes. To determine the effects of this exoribonuclease in development we have constructed a number of mutations in <jats:italic>pacman<\/jats:italic> by P\u2010element excision and characterized the resulting phenotypes.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Mutations in <jats:italic>pacman<\/jats:italic> resulted in flies with a number of specific phenotypes, such as low viability, dull wings, crooked legs, failure of correct dorsal\/thorax closure and defects in wound healing. The epithelial sheet movement involved in dorsal\/thorax closure is a conserved morphogenetic process which is similar to that of hind\u2010brain closure in vertebrates and wound healing in humans. As the JNK (c\u2010Jun N\u2010terminal kinase) signalling pathway is known to be involved in dorsal\/thorax closure and wound healing, we tested whether <jats:italic>pacman<\/jats:italic> affects JNK signalling. Our experiments demonstrate that <jats:italic>pacman<\/jats:italic> genetically interacts with <jats:italic>puckered<\/jats:italic>, a phosphatase that negatively regulates the JNK signalling pathway.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. These results reveal that the 5\u2032\u20133\u2032 exoribonuclease <jats:italic>pacman<\/jats:italic> is required for a critical aspect of epithelial sheet sealing in <jats:italic>Drosophila<\/jats:italic>. Since these mutations result in specific phenotypes, our data suggest that the exoribonuclease Pacman targets a specific subset of mRNAs involved in this process. One of these targets could be a member of the JNK signalling pathway, although it is possible that a parallel pathway may instead be affected. The exoribonuclease <jats:italic>pacman<\/jats:italic> is highly conserved in all eukaryotes, therefore it is likely that it is involved in similar morphological processes, such as wound healing in human cells.<\/jats:p>","DOI":"10.1042\/bc20080049","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T13:35:00Z","timestamp":1213191300000},"page":"687-701","source":"Crossref","is-referenced-by-count":21,"title":["The 5\u2032\u20133\u2032 exoribonuclease <i>pacman<\/i> is required for epithelial sheet sealing in <i>Drosophila<\/i> and genetically interacts with the phosphatase <i>puckered<\/i>"],"prefix":"10.1111","volume":"100","author":[{"given":"Dominic P.","family":"Grima","sequence":"first","affiliation":[]},{"given":"Melanie","family":"Sullivan","sequence":"additional","affiliation":[]},{"given":"Maria 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