{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:26:11Z","timestamp":1771633571449,"version":"3.50.1"},"reference-count":51,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,7,15]]},"abstract":"<jats:p>ATP is released from pancreatic acini in response to cholinergic and hormonal stimulation. The same stimuli cause exocytosis of ZG (zymogen granules) and release of digestive enzymes. The aim of the present study was to determine whether ZG stored ATP and to characterize the uptake mechanism for ATP transport into the ZG. ZG were isolated and the ATP content was measured using luciferin\/luciferase assays and was related to protein in the sample. The estimate of ATP concentration in freshly isolated granules was 40\u2013120\u00a0\u03bcM. The ATP uptake had an apparent Km value of 4.9\u00b12.1\u00a0mM when granules were incubated without Mg2+ and a Km value of 0.47\u00b10.05\u00a0mM in the presence of Mg2+, both in pH\u00a06.0 buffers. The uptake of ATP was significantly higher at pH\u00a07.2 compared with pH\u00a06.0 solutions. The anion transport blockers DIDS (4,4\u2032-di-isothiocyanostilbene-2,2\u2032-disulfonate) and Evans Blue inhibited ATP transport. Western blot analysis on the ZG showed the presence of VNUT (vesicular nucleotide transporter). Together, these findings indicate that VNUT may be responsible for the ATP uptake into ZG. Furthermore, the present study shows the presence of ATP together with digestive enzymes in ZG. This indicates that co-released ATP would regulate P2 receptors in pancreatic ducts and, thus, ductal secretion, and this would aid delivery of enzymes to the duodenum.<\/jats:p>","DOI":"10.1042\/bj20091337","type":"journal-article","created":{"date-parts":[[2010,5,5]],"date-time":"2010-05-05T14:01:30Z","timestamp":1273068090000},"page":"303-311","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["ATP storage and uptake by isolated pancreatic zymogen granules"],"prefix":"10.1042","volume":"429","author":[{"given":"Kristian\u00a0A.","family":"Haanes","sequence":"first","affiliation":[{"name":"Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Novak","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Copenhagen, Universitetsparken 13, DK-2100 Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2010,6,28]]},"reference":[{"key":"2021112214355788000_B1","doi-asserted-by":"crossref","first-page":"32925","DOI":"10.1074\/jbc.M103313200","article-title":"Visualization of ATP release in pancreatic acini in response to cholinergic stimulus: use of fluorescent probes and confocal microscopy","volume":"276","author":"Sorensen","year":"2001","journal-title":"J. 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