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The <jats:italic>N<\/jats:italic>\u2010ethyl\u20105\u2010phenylisoxazolium\u20103\u2032\u2010sulphonate (Woodward reagent K) gave a high binding but totally inhibited the enzyme activity. Best results were obtained with CM\u2010HASCL\u201035 using 1\u2010ethyl\u20103\u2010(3\u2010dimethylaminopropyl)carbodi\u2010imide as a coupling agent. The immobilized enzyme retained 88% of its initial limit rate [<jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub>(app)=16 EU\/mg for immobilized uricase versus <jats:italic>V<\/jats:italic><jats:sub>max<\/jats:sub>=18 EU\/mg for the free enzyme], with an apparent decrease of affinity for urate substrate [<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>(app)=0.17 mM versus <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>=0.03 mM for the free enzyme]. The coupling yield was 60% and the modified uricase was found more resistant to proteolysis than the free enzyme. The immobilized uricase retained 25% of its initial activity after 60 min in pancreatic proteolysis medium (pancreatin), whereas the free enzyme retained only 5% of its initial activity. The best immobilization yield was obtained with the polymeric support based on CM\u2010HASCL\u201035 (53%), which gave better results than commercial supports based on agarose.<\/jats:p>","DOI":"10.1042\/ba20020028","type":"journal-article","created":{"date-parts":[[2003,12,8]],"date-time":"2003-12-08T15:18:38Z","timestamp":1070896718000},"page":"163-170","source":"Crossref","is-referenced-by-count":8,"title":["Modified high amylose starch for immobilization of uricase for therapeutic application"],"prefix":"10.1002","volume":"36","author":[{"given":"J\u00e9rome","family":"Mulhbacher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"McGeeney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pompilia","family":"Ispas\u2010Szabo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Lenaerts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mircea\u2010Alexandru","family":"Mateescu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400850"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb18151.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780080429"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.55.639.26"},{"key":"e_1_2_6_6_2","first-page":"22","volume":"3","author":"Terkeltaub R.","year":"1996","journal-title":"Sci. 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