{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:50:22Z","timestamp":1791478222002,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":2944,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>The mean molecular masses of three different meningococcal C saccharide (MenC)\u2013protein conjugate vaccines and their constituent proteins were estimated using HPLC size\u2010exclusion chromatography (SEC) with multi\u2010angle laser light scattering (MALLS) and refractive\u2010index (RI) detection (SEC\/MALLS). Chromatography of two CRM<jats:sub>197<\/jats:sub> conjugates (MenC\u2013CRM<jats:sub>197<\/jats:sub>\u2010A and MenC\u2013CRM<jats:sub>197<\/jats:sub>\u2010B) and one tetanus toxoid (TT) conjugate (MenC\u2013TT) was performed in PBS, pH 7.4, on TSK\u2010Gel\u00ae (TosoHaas) analytical columns [CRM<jats:sub>197<\/jats:sub> is a non\u2010catalytic cross\u2010reacting mutant (CRM) of diphtheria toxin]. Analysis of the light\u2010scattering signal measured at 18 angles simultaneously, using the RI signal as a measure of concentration, gave absolute weight\u2010average\u2010molecular\u2010mass (<jats:italic>&amp;#x004d;\u0304<\/jats:italic><jats:sub><jats:italic><jats:bold>w<\/jats:bold><\/jats:italic><\/jats:sub>) values for the CRM<jats:sub>197<\/jats:sub> conjugates as follows: MenC\u2010CRM<jats:sub>197<\/jats:sub>\u2010A, \u224875000 g\u00b7mol<jats:sup>\u22121<\/jats:sup> and MenC\u2013CRM<jats:sub>197<\/jats:sub>\u2010B, \u2248350000 g\u00b7mol<jats:sup>\u22121<\/jats:sup>, suggesting that MenC\u2013CRM<jats:sub>197<\/jats:sub>\u2010A is a monomer (one carrier protein per conjugate molecule), while MenC\u2010CRM<jats:sub>197<\/jats:sub>\u2010B is largely composed of conjugates containing three or four CRM<jats:sub>197<\/jats:sub> molecules. The MenC\u2013TT conjugate eluted as a two\u2010component system with <jats:italic>&amp;#x004d;\u0304<\/jats:italic><jats:sub><jats:italic><jats:bold>w<\/jats:bold><\/jats:italic><\/jats:sub> of 1.63\u00d710<jats:sup>6<\/jats:sup> and 395000 g\u00b7 mol<jats:sup>\u22121<\/jats:sup>, suggesting that some cross\u2010linked complexes contain up to six TT molecules. Comparison of results from MALLS\/RI with those obtained using UV detection highlights the differences in size and relative composition of the various subpopulations of the MenC conjugates that can be obtained using different detection systems.<\/jats:p>","DOI":"10.1042\/ba20020066","type":"journal-article","created":{"date-parts":[[2003,12,8]],"date-time":"2003-12-08T15:18:38Z","timestamp":1070896718000},"page":"219-226","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of meningococcal C oligosaccharide conjugate vaccines by size\u2010exclusion chromatography\/multi\u2010angle laser light scattering"],"prefix":"10.1002","volume":"36","author":[{"given":"Kornelia","family":"Jumel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei M.","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Bolgiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.302-317.2000"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03594-7"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1128\/iai.40.1.245-256.1983","volume":"40","author":"Chu C.","year":"1983","journal-title":"Infect. 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