{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T08:15:59Z","timestamp":1758701759023},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":2517,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p>Previously we have reported the capacity of the fusion protein PD3, composed of the P64k protein and the envelope (E) fragment from amino acids (aa) 286\u2013426 of dengue\u20102 virus (DEN\u20102), to induce a functional immune response in mice against the homologous virus. In that case, the E fragment was inserted within the lipoyl\u2010binding domain of the meningococcal P64k protein. In the present study, to test the functionality of the same E region from dengue\u20101 (DEN\u20101), a similar construct was made. Furthermore, another alternative of fusion protein was also constructed where the same E fragment from DEN\u20101 was fused to the C\u2010terminus of the P64k protein. The recombinant proteins obtained (PD11 and PD10) were semi\u2010purified and analysed for their antigenicity, immunogenicity and the ability to protect mice against lethal challenge. Both molecules exhibited the same recognition patterns against anti\u2010DEN\u20101 polyclonal antibodies. In addition, when administered to mice, they elicited high levels of neutralizing antibodies and induced significant protection against lethal challenge with DEN\u20101 after intracerebral inoculation. These results reveal the availability of two sites within the P64k for the further insertion of DEN fragments, enabling a construct carrying two fragments from heterologous serotypes within the same molecule of this protein carrier.<\/jats:p>","DOI":"10.1042\/ba20030039","type":"journal-article","created":{"date-parts":[[2008,12,4]],"date-time":"2008-12-04T11:04:23Z","timestamp":1228388663000},"page":"107-114","source":"Crossref","is-referenced-by-count":24,"title":["A fragment of the envelope protein from dengue\u20101 virus, fused in two different sites of the meningococcal P64k protein carrier, induces a functional immune response in mice"],"prefix":"10.1002","volume":"39","author":[{"given":"Lisset","family":"Hermida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayner","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Lazo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00eddice","family":"Bernardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A\u00edda","family":"Zulueta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Vald\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delfina","family":"Del Rosario","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Guill\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda G.","family":"Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.003245"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00171-2"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.5.1105"},{"key":"e_1_2_7_5_2","first-page":"279","volume-title":"Sructure of the Flavivirus Genome","author":"Rice C. M.","year":"1986"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"2547","DOI":"10.1128\/JVI.73.3.2547-2551.1999","volume":"73","author":"Wang S. H. E.","year":"1999","journal-title":"J. Virol."},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00128-X"},{"key":"e_1_2_7_8_2","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1099\/0022-1317-78-8-1861","volume":"78","author":"Sugrue R. J.","year":"1997","journal-title":"J. Virol."},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(01)00406-0"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1991.tb02026.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)00059-V"},{"key":"e_1_2_7_12_2","doi-asserted-by":"crossref","first-page":"655","DOI":"10.4269\/ajtmh.1998.58.655","volume":"58","author":"Simmons M.","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_2_7_13_2","doi-asserted-by":"crossref","first-page":"500","DOI":"10.4269\/ajtmh.1991.44.500","volume":"44","author":"Fonseca B. A. L.","year":"1991","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_2_7_14_2","unstructured":"Hermida L. Rodr\u00edguez R. Lazo L. Zulueta A. L\u00f3pez C. Vald\u00e9s I. Silva R. Chinea G. Guill\u00e9n G. Guzm\u00e1n M. G. Sierra B.andEspinosa R.(2002) Patent Applic. PCT\/Cu02\/00006"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1470-8744.1998.tb00494.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/BA20010029"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008261031034"},{"key":"e_1_2_7_18_2","first-page":"376","volume":"4","author":"Bullock W. O.","year":"1987","journal-title":"Biotechniques"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(77)90289-3"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1958.7.561"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-40-3-531"},{"key":"e_1_2_7_22_2","first-page":"203","volume":"18","author":"Rosario D.","year":"2001","journal-title":"Biotecnol. Apl."},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_7_24_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90101-2"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_7_28_2","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1128\/jcm.22.2.250-254.1985","volume":"22","author":"Morens D. M.","year":"1985","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_7_29_2","unstructured":"Hermida L. Rodr\u00edguez R. Lazo L. Silva R. Zulueta A. Chinea G. L\u00f3pez C. Guzm\u00e1n M. G.andGuill\u00e9n G.(2003)J. Virol. Methods in the press"},{"key":"e_1_2_7_30_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4269\/ajtmh.2001.65.159","volume":"65","author":"Simmons M.","year":"2001","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_2_7_31_2","doi-asserted-by":"crossref","first-page":"4356","DOI":"10.1128\/jvi.64.9.4356-4363.1990","volume":"64","author":"Falgout B.","year":"1990","journal-title":"J. Virol."},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.17.8094-8101.2000"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg009"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FBA20030039","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/BA20030039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:52:50Z","timestamp":1697244770000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1042\/BA20030039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,2]]}},"alternative-id":["10.1042\/BA20030039"],"URL":"https:\/\/doi.org\/10.1042\/ba20030039","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2]]}}}