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We have reported previously that immunization with <jats:italic>N. lactamica<\/jats:italic> outer\u2010membrane vesicles containing the major OMPs (outer\u2010membrane proteins) protected mice against lethal challenge with meningococci of diverse serogroups and serotypes and has the potential to form the basis of a vaccine against meningococcal diseases [Oliver, Reddin, Bracegirdle et al. (2002) Infect. Immun. 70, 3621\u20133626]. In the present study, we have shown that biomass production and the profile of outer\u2010membrane vesicle proteins may be affected by fermentation conditions and, in particular, media composition. Ciphergen SELDI\u2013TOF Protein Chips\u00ae were used as a rapid and sensitive new method in comparison with conventional SDS\/PAGE. SELDI\u2013TOF\u2010MS (surface\u2010enhanced laser\u2010desorption ionization\u2013time\u2010of\u2010flight MS) reproducibly identified three major OMPs (NspA, RmpM and PorB) and detected the changes in the protein profile when the growth medium was altered. The findings of this work indicate that SELDI\u2013TOF\u2010MS is a useful tool for the rapid optimization of OMP production in industrial fermentation processes and can be adapted as a Process Analytical Technology (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fda.gov\/cder\/OPS\/PAT.htm\">http:\/\/www.fda.gov\/cder\/OPS\/PAT.htm<\/jats:ext-link>).<\/jats:p>","DOI":"10.1042\/ba20040098","type":"journal-article","created":{"date-parts":[[2005,3,22]],"date-time":"2005-03-22T22:06:13Z","timestamp":1111529173000},"page":"175-182","source":"Crossref","is-referenced-by-count":23,"title":["Rapid characterization of outer\u2010membrane proteins in <i>Neisseria lactamica<\/i> by SELDI\u2013TOF\u2010MS (surface\u2010enhanced laser desorption ionization\u2013time\u2010of\u2010flight MS) for use in a meningococcal vaccine"],"prefix":"10.1002","volume":"41","author":[{"given":"Tarit Kumar","family":"Mukhopadhyay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Halliwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cliona","family":"O'Dwyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz Ayazi","family":"Shamlou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam Susana","family":"Levy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel","family":"Allison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Gorringe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen M.","family":"Reddin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.129.6.1327"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2002.tb00608.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00299-7"},{"key":"e_1_2_7_5_2","unstructured":"Eradicating Meningococcal Disease in Young People: Progress Report by the Chief Medical Officer Sir Liam Donaldson Department of Health London(http:\/\/www.publications.doh.gov.uk\/cmo\/progress\/meningitis\/)"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/150.1.71"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.5010-5015.2001"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(93)90045-Y"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1561-1567.2001"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.7.1173"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00774-0"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1650-1655.2003"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(96)00020-5"},{"key":"e_1_2_7_14_2","doi-asserted-by":"crossref","first-page":"2552","DOI":"10.1128\/IAI.67.5.2552-2560.1999","volume":"67","author":"Lehmann A. 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