{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T09:54:55Z","timestamp":1760954095963},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":1513,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p>A conjugate meningococcal vaccine against serogroup B\/C consisting of capsular PS (polysaccharide) from serogroup C conjugated to OMV (outer membrane vesicle) from serogroup B would be a very useful vaccine in regions where there is a prevalence of both serogroups, for example in Brazil. For this purpose, the conjugation method that uses ADHy (adipic acid dihydrazide) as spacer and a carbodi\u2010imide derivative, EDAC [1\u2010ethyl\u20103\u2010(3\u2010dimethylaminopropyl)carbodi\u2010imide], as catalyser was optimized looking for synthesis yield and maintenance of the antigenicity of both components. The best synthesis conditions preserving the vaccine immunogenicity resulted in a final yield of approx. 17%. Immunogenicity of the vaccine was highest when 10% of the sialic acid residues of the PS were occupied by the ADHy spacer. Sterilization of the conjugate by filtration through a 0.22\u2010\u03bcm\u2010pore\u2010size membrane resulted in a low recovery of protein and PS (\u223c50%), although the vaccine immunogenicity was maintained. Using \u03b3 irradiation on freeze\u2010dried sample, it was possible to maintain the integrity of OMV structure and, consequently, its ability to induce bactericidal antibodies.<\/jats:p>","DOI":"10.1042\/ba20060041","type":"journal-article","created":{"date-parts":[[2006,10,19]],"date-time":"2006-10-19T10:41:31Z","timestamp":1161254491000},"page":"141-146","source":"Crossref","is-referenced-by-count":6,"title":["Optimization of the conjugation method for a serogroup B\/C meningococcal vaccine"],"prefix":"10.1002","volume":"45","author":[{"given":"Lucila O.","family":"Fukasawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rocilda P. 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