{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T16:29:05Z","timestamp":1789144145484,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":1087,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>In the present study, the growth and key metabolic features of a gene\u2010transfected <jats:italic>Drosophila melanogaster<\/jats:italic> (fruitfly) S2 (Schneider 2) cell population (S2AcRVGP cells), cultured in Sf900\u2010II medium, have been evaluated to provide substantial support for the development of a bioprocess to produce RVGP (rabies\u2010virus glycoprotein). Experimental cultures were grown both in a 100 ml Schott flask incubated in a shaker at 28 \u00b0C and 100 rev.\/min and in a 3 litre stirred\u2010tank bioreactor at 28 \u00b0C, with increasing agitation. In small\u2010scale culture, S2AcRVGP cells reached a maximum cell concentration of 1.13\u00d710<jats:sup>7<\/jats:sup> cell\/ml, presented a \u03bc<jats:sub>max<\/jats:sub> (maximum specific growth rate) of 0.037 h<jats:sup>\u22121<\/jats:sup> and the growth was limited by oxygen deprivation. An early and remarkably long stationary phase was observed under hypoxia. Cell cultures grown in the bioreactor without oxygen limitation exhibited a maximum cell concentration of 2.2\u00d710<jats:sup>7<\/jats:sup> cells\/ml and \u03bc<jats:sub>max<\/jats:sub> values as high as 0.048 h<jats:sup>\u22121<\/jats:sup>. The main substrate consumed in order to reach such a high growth rate was the amino acid proline, which seems to play an important role as a source of metabolic energy in the culture of S2AcRVGP cells. Under conditions of hypoxia, the cells were able to survive for 15 h without apparent damage, recovering their previous metabolic activity.<\/jats:p>","DOI":"10.1042\/ba20060148","type":"journal-article","created":{"date-parts":[[2007,12,18]],"date-time":"2007-12-18T16:50:17Z","timestamp":1197996617000},"page":"41-49","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of growth and metabolism of <i>Drosophila melanogaster<\/i> cells transfected with the rabies\u2010virus glycoprotein gene"],"prefix":"10.1002","volume":"49","author":[{"given":"Kamilla","family":"Swiech","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00f3vis S.","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mabel K.","family":"Arantes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra S.","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato M.","family":"Astray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udio A. 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