{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:59:16Z","timestamp":1761217156057},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":1087,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>T\u03b11 (thymosin \u03b1 1) is important in treating immunodeficiency and other diseases. In order to study the feasibility of expressing T\u03b11 in plants, as the first attempt, we designed and synthesized the <jats:italic>T<\/jats:italic>\u03b1<jats:italic>1<\/jats:italic> gene according to the plant codon usage preference and constructed the 4\u00d7T\u03b11 concatemer (four copies of a DNA sequence arranged end\u2010to\u2010end in tandem). The latter was inserted into <jats:italic>Escherichia coli<\/jats:italic> expression vector pQE30, resulting in a recombinant plasmid that was subsequently transformed into <jats:italic>E. coli<\/jats:italic> M15. The 4\u00d7T\u03b11 concatemer protein was successfully expressed in <jats:italic>E. coli<\/jats:italic> in a soluble form. The expressed protein was purified and its bioactivity was analysed by MTT [3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl\u20102<jats:italic>H<\/jats:italic>\u2010tetrazolium bromide] assay. Preliminary results showed that the 4\u00d7T\u03b11 concatemer protein could stimulate the mice spleen lymphocyte proliferation. This is the first report on the expression of 4\u00d7T\u03b11 concatemer that was synthesized according to plant codon usage preference in an <jats:italic>E. coli<\/jats:italic> expression system. The present study provides the basis for expressing the synthesized active <jats:italic>T<\/jats:italic>\u03b1<jats:italic>1<\/jats:italic> gene in plants in the future.<\/jats:p>","DOI":"10.1042\/ba20070055","type":"journal-article","created":{"date-parts":[[2007,12,18]],"date-time":"2007-12-18T16:50:17Z","timestamp":1197996617000},"page":"51-56","source":"Crossref","is-referenced-by-count":11,"title":["Expression and analysis of thymosin \u03b11 concatemer in <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"49","author":[{"given":"Yuhui","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingxia","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoan","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijie","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwei","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexuan","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.2.725"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7270.2005.00014.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0192-0561(00)00075-8"},{"key":"e_1_2_6_5_2","first-page":"698","volume":"3","author":"Billich A.","year":"2002","journal-title":"Curr. Opin. Investig. Drugs"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00567a023"},{"key":"e_1_2_6_7_2","first-page":"55","volume":"24","author":"Shi J. H.","year":"2003","journal-title":"Chin. J. Biochem. Pharm."},{"key":"e_1_2_6_8_2","first-page":"52","volume":"30","author":"Xu F.","year":"2001","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"e_1_2_6_9_2","first-page":"541","volume":"18","author":"Xiu Z. Y.","year":"2002","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1517\/14728214.10.1.185"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.2.477"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_6_13_2","first-page":"245","volume":"21","author":"Chen Z. Y.","year":"2000","journal-title":"Chin. J. Biochem. Pharm."},{"key":"e_1_2_6_14_2","first-page":"465","volume":"18","author":"Macreadie I. G.","year":"1998","journal-title":"Recent Res. Dev. Biotech. Bioeng."},{"key":"e_1_2_6_15_2","first-page":"287","volume":"18","author":"Hu X. J.","year":"2002","journal-title":"Chin. J. Biochem. Mol. Biol."},{"key":"e_1_2_6_16_2","first-page":"67","volume":"8","author":"Zhao Y. T.","year":"2001","journal-title":"Pharm. Biotechnol."},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-004-7333-3"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FBA20070055","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/BA20070055","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T10:06:47Z","timestamp":1697105207000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1042\/BA20070055"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1042\/BA20070055"],"URL":"https:\/\/doi.org\/10.1042\/ba20070055","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}