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Other problems include difficulties in antigen production and the associated biohazard risk. This has prompted the need to develop an alternative antigen to replace LPS. In the present study, we cloned and expressed a BP26 (26 kDa periplasmic protein) antigen gene (<jats:italic>bp26<\/jats:italic>) of <jats:italic>Brucella abortus<\/jats:italic>. The recombinant periplasmic protein [rBP26 (recombinant BP26)] was expressed to high levels in <jats:italic>Escherichia coli<\/jats:italic> and purified in a single step. The purified rBP26 was examined for its binding activity with antibodies in a serum derived from a rabbit immunized intramuscularly with whole\u2010cell lysate of <jats:italic>B. abortus<\/jats:italic>, as well as with commercial <jats:italic>Brucella<\/jats:italic> antibody (Difco). The purified rBP26 was used to develop an in\u2010house plate ELISA and was further tested with a panel of 75 bovine brucellosis sera samples characterized previously by conventional serological tests. The results of both were in excellent agreement. The results show that rBP26 has potential use in the diagnosis of brucellosis, both in the laboratory and in field\u2010based conditions with high levels of sensitivity and specificity.<\/jats:p>","DOI":"10.1042\/ba20070111","type":"journal-article","created":{"date-parts":[[2008,2,18]],"date-time":"2008-02-18T11:26:19Z","timestamp":1203333979000},"page":"213-218","source":"Crossref","is-referenced-by-count":14,"title":["Expression and purification of the 26 kDa periplasmic protein of <i>Brucella abortus<\/i>: a reagent for the diagnosis of bovine brucellosis"],"prefix":"10.1002","volume":"49","author":[{"given":"Sanjay","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urmil","family":"Tuteja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harsh Vardhan","family":"Batra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"301","volume-title":"Animal Brucellosis","author":"Enright F. 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