{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:20:09Z","timestamp":1790317209879,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":843,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p>Previous studies have shown that <jats:italic>Dehalococcoides<\/jats:italic> species are responsible for the anaerobic bioremediation of chloroethene pollution. It has been thought that co\u2010operation of several species is required for complete dechlorination to ethene. In the present study, we used quantitative PCR of 16 S rRNA and RDase (reductive dehalogenase) genes to examine species changes and the population of <jats:italic>Dehalococcoides<\/jats:italic> species in ground water in which the dechlorination of TCE (trichloroethene) to ethene was enhanced by delivery of hydrogen\u2010releasing compounds. The results have shown that at least two different <jats:italic>Dehalococcoides<\/jats:italic> species co\u2010operate in the dechlorination of TCE to ethene. Initially, the number of strains equipped with TCE RDase increased approx. 10<jats:sup>5<\/jats:sup>\u2010fold. This was followed by a decrease to the original level, according to the exhaustion of TCE. Subsequently, another strain appeared, which had a VC (vinyl chloride) RDase gene similar to <jats:italic>bvcA<\/jats:italic> of <jats:italic>Dehalococcoides<\/jats:italic> sp. BAV1 and is probably responsible for the dechlorination of VC to ethene. Analysis of several genes has suggested that the former strain is like <jats:italic>Dehalococcoides<\/jats:italic> sp. FMC\u2010TCE, and the latter strain is similar to the <jats:italic>Dehalococcoides<\/jats:italic> sp. strain that exists in the <jats:italic>Dehalococcoides<\/jats:italic>\u2010containing mixed culture KB1. These results support the notion that monitoring <jats:italic>Dehalococcoides<\/jats:italic> species by the presence of RDase genes as genetic markers provides detailed information on the progress of bioremediation of chloroethenes, which will be useful to improve the efficiency of bioremediation.<\/jats:p>","DOI":"10.1042\/ba20070171","type":"journal-article","created":{"date-parts":[[2008,8,18]],"date-time":"2008-08-18T10:16:57Z","timestamp":1219054617000},"page":"1-7","source":"Crossref","is-referenced-by-count":15,"title":["Detection and identification of <i>Dehalococcoides<\/i> species responsible for <i>in situ<\/i> dechlorination of trichloroethene to ethene enhanced by hydrogen\u2010releasing compounds"],"prefix":"10.1002","volume":"51","author":[{"given":"Minoru","family":"Nishimura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiko","family":"Ebisawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakari","family":"Sakihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsushi","family":"Nakama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Okochi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Yohda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.00108579"},{"key":"e_1_2_6_3_2","first-page":"14","volume-title":"Bioremediation of Chlorinated and Polycyclic Aromatic Hydrocarbon Compounds","author":"Beeman R.","year":"1994"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.56.3.482-507.1992"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.9.2991-2997.1993"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1128\/aem.62.3.761-765.1996","volume":"62","author":"Sharma P. 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