{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T06:10:47Z","timestamp":1697177447413},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":690,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:p>PA (protective antigen) is a major immunogen of the vaccine against anthrax. In the present study, a new expression system, <jats:italic>Escherichia coli<\/jats:italic> strain Rosetta\u2122 2(DE3), was used for high\u2010level expression of rPA (recombinant PA) whose gene contains 66 rare <jats:italic>E. coli<\/jats:italic> codons (9.0% of 733 total PA gene codons). The rPA\u2010formed inclusion bodies were washed with Triton X\u2010100 and 2\u00a0M urea and solubilized in 5\u00a0M urea, followed by a 60%\u2010satd.\u2010ammonium sulfate precipitation. Finally, the untagged rPA was efficiently and rapidly purified by single\u2010step hydrophobic\u2010interaction chromatography using Phenyl\u2010Sepharose High Performance resin on an AKTA Purifier 10 system. The yield was approx. 13\u00a0mg of high\u2010purity (&gt;99%) biologically active rPA per litre of culture, which can be used for the detection of anthrax and as a potential component of a vaccine against anthrax.<\/jats:p>","DOI":"10.1042\/ba20070245","type":"journal-article","created":{"date-parts":[[2008,5,8]],"date-time":"2008-05-08T11:58:08Z","timestamp":1210247888000},"page":"107-112","source":"Crossref","is-referenced-by-count":6,"title":["High\u2010level expression and single\u2010step purification of recombinant <i>Bacillus anthracis<\/i> protective antigen from <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"52","author":[{"given":"Jinbiao","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yefu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guozhi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(88)65019-1"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-0090-x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1128\/iai.39.1.371-376.1983","volume":"39","author":"Mikesell P.","year":"1983","journal-title":"Infect. 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