{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:01:48Z","timestamp":1773277308823,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":690,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:p>The antiproliferative effects of 15\u2010LOX (15\u2010lipoxygenase) metabolites of arachidonic acid {(15<jats:italic>S<\/jats:italic>)\u2010HPETE [(15<jats:italic>S<\/jats:italic>)\u2010hydroperoxyeicosatetraenoic acid] and (15<jats:italic>S<\/jats:italic>)\u2010HETE [(15<jats:italic>S<\/jats:italic>)\u2010hydroxyeicosatetraenoic acid]} and the mechanism(s) involved were studied in the human T\u2010cell leukaemia cell line Jurkat. (15<jats:italic>S<\/jats:italic>)\u2010HPETE, the hydroperoxy metabolite of 15\u2010LOX, inhibited the growth of Jurkat cells 3\u00a0h after exposure and with an IC<jats:sub>50<\/jats:sub> value of 10\u00a0\u03bcM. The hydroxy metabolite of 15\u2010LOX, (15<jats:italic>S<\/jats:italic>)\u2010HETE, on the other hand, inhibited the growth of Jurkat cells after 6\u00a0h of exposure and with an IC<jats:sub>50<\/jats:sub> value of 40\u00a0\u03bcM. The cells exposed to 10\u00a0\u03bcM (15<jats:italic>S<\/jats:italic>)\u2010HPETE for 3\u00a0h or to 40\u00a0\u03bcM (15<jats:italic>S<\/jats:italic>)\u2010HETE for 6\u00a0h showed increased expression of Fas ligand and FADD (Fas\u2010associated death domain), caspase 8 activation, Bid (BH3\u2010interacting domain death agonist) cleavage, decrease in mitochondrial membrane potential, cytochrome <jats:italic>c<\/jats:italic> release, caspase 3 activation, PARP\u20101 [poly(ADP\u2010ribose) polymerase\u20101] cleavage and DNA fragmentation, suggesting the involvement of both extrinsic and intrinsic death pathways. Further studies on ROS (reactive oxygen species) generation revealed the involvement of NADPH oxidase. In conclusion, the present study indicates that NADPH oxidase\u2010induced ROS generation activates the Fas\u2010mediated death pathway.<\/jats:p>","DOI":"10.1042\/ba20070264","type":"journal-article","created":{"date-parts":[[2009,1,6]],"date-time":"2009-01-06T09:48:59Z","timestamp":1231235339000},"page":"121-133","source":"Crossref","is-referenced-by-count":15,"title":["Effects of (15<i>S<\/i>)\u2010hydroperoxyeicosatetraenoic acid and (15<i>S<\/i>)\u2010hydroxyeicosatetraenoic acid on the acute\u2010 lymphoblastic\u2010leukaemia cell line Jurkat: activation of the Fas\u2010mediated death pathway"],"prefix":"10.1002","volume":"52","author":[{"given":"Kotha\u00a0Anil","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalle\u00a0M.","family":"Arunasree","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karnati\u00a0R.","family":"Roy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishanth\u00a0P.","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankireddy","family":"Aparna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gorla\u00a0Venkateswara","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pallu","family":"Reddanna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-5323(03)00035-6"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5239.1189"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/377630a0"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(92)90297-9"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(96)00008-2"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508260200"},{"key":"e_1_2_8_8_2","first-page":"6307","volume":"61","author":"Shureiqi I.","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_8_9_2","first-page":"360","volume":"59","author":"Ikawa H.","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.10.1777"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/20.10.1985"},{"key":"e_1_2_8_12_2","first-page":"497","volume":"61","author":"Shappell S. 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