{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:59:43Z","timestamp":1790719183272,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":236,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:p>The use of the recombinant BoNT\/A\u2010Hc (carboxylic domain of the <jats:italic>Clostridium botulinum<\/jats:italic> neurotoxin heavy chain) has been proposed as a vaccine candidate for botulism. This fragment contains the principle protective antigenic determinant. In the present study, in order to maximize recombinant protein expression, after verification of recombinant BoNT\/A\u2010Hc by Western blotting, modified M9 medium was selected as a simple medium, and the operational and medium\u2010composition variables together with their interactions were optimized by using the Taguchi statistical method. ANOVA for the obtained data indicated that 3.5 g, 15 g, 30 g, 15 g, 4 g, 0.7 mM, 1.5 ml per litre of medium, 30 \u00b0C and 15 h represented optimum values of (NH<jats:sub>4<\/jats:sub>)<jats:sub>2<\/jats:sub>SO<jats:sub>4<\/jats:sub>, glucose, K<jats:sub>2<\/jats:sub>HPO<jats:sub>4<\/jats:sub>, KH<jats:sub>2<\/jats:sub>PO<jats:sub>4<\/jats:sub>, MgSO<jats:sub>4<\/jats:sub>\u00b77H<jats:sub>2<\/jats:sub>O, isopropyl \u03b2\u2010<jats:sc>D<\/jats:sc>\u2010thiogalactoside concentration, trace\u2010elements solution, temperature and post\u2010induction time respectively. Consequently, under these optimum conditions, 52.1 mg\/l of soluble BoNT\/A\u2010Hc was obtained in shake flask culture.<\/jats:p>","DOI":"10.1042\/ba20090315","type":"journal-article","created":{"date-parts":[[2010,4,26]],"date-time":"2010-04-26T10:00:35Z","timestamp":1272276035000},"page":"35-42","source":"Crossref","is-referenced-by-count":15,"title":["Optimization of the BoNT\/A\u2010Hc expression in recombinant <i>Escherichia coli<\/i> using the Taguchi statistical method"],"prefix":"10.1002","volume":"56","author":[{"given":"Kheirollah","family":"Yari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Safa\u2010ali","family":"Fatemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Tavallaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2008.12.055"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.2.717"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.2000.1227"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.8.5524"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.16.1.19"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00072-T"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.06.094"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.570-574.2001"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2006.09.003"},{"key":"e_1_2_8_11_2","first-page":"91","volume":"27","author":"Khosravi Darani K.","year":"2008","journal-title":"Iran. J. Bioeng."},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.3923\/biotech.2007.456.464"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2006.05.019"},{"key":"e_1_2_8_14_2","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.5897\/AJB2007.000-2408","volume":"6","author":"Ghaemi Oskouie S. F.","year":"2007","journal-title":"Afr. J. Biotechnol."},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(03)00207-3"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-4530.2007.00112.x"},{"key":"e_1_2_8_17_2","first-page":"295","volume":"12","author":"Jun H. J.","year":"1998","journal-title":"BioTechniques"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000004390.98648.25"},{"key":"e_1_2_8_19_2","first-page":"96","volume":"3","author":"Motallebi M.","year":"2008","journal-title":"World Appl. Sci. J."},{"key":"e_1_2_8_20_2","first-page":"159","volume-title":"Optimisation of the solubility of the recombinant Itk kinase domain in Escherichia coli Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: a Comparative View on Host Physiology","author":"Meinander N. Q.","year":"2000"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-006-9158-9"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-004-5841-x"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0032-9592(02)00224-8"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2004.06.017"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1999.1138"},{"key":"e_1_2_8_26_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.06.058"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-006-9034-6"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.01.052"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/s7030371"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FBA20090315","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/BA20090315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T04:44:44Z","timestamp":1698727484000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1042\/BA20090315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1042\/BA20090315"],"URL":"https:\/\/doi.org\/10.1042\/ba20090315","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5]]}}}