{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:04:49Z","timestamp":1760645089447},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":2321,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. The hormone PYY (peptide YY), synthesized by endocrine cells in the pancreas, ileum, colon and stomach has widespread inhibitory effects on gastrointestinal and pancreatic fluid secretion. Transgenic mice expressing a viral oncoprotein under the control of the PYY gene 5\u2032\u2010flanking region develop well\u2010differentiated colonic endocrine tumours producing mainly PYY and enteroglucagon. In the present study, we investigated the expression of AQP4 (aquaporin 4) water channel and H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase in stomachs from both control and transgenic mice.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Semi\u2010quantitative RT (reverse transcriptase)\u2013PCR showed an increase in the AQP4 transcript compared with control mice. Quantitative Western\u2010blot analysis of stomachs from control and transgenic mice confirmed a significant increase in the 30 kDa AQP4 protein in transgenic mice. In control mice, AQP4 is specifically expressed in the basolateral membrane of gastric parietal cells, located in the basal region of the fundic glands. This particular location suggests that parietal cells in the base region of gastric pits might have a major role in water transport when compared with the more superficial parietal cells. Interestingly, immunofluorescence studies on transgenic mice revealed that the quantitative increase of AQP4 expression was actually due to an increase in the number of AQP4\u2010expressing epithelial cells rather than to a higher expression of AQP4 in parietal cells. In fact, immunofluorescence experiments using the specific antibody raised against the AE2 isoform of Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchanger specifically expressed in parietal cells confirmed that the number of parietal cells was comparable in both PYY and control stomachs. Moreover, in transgenic mice, a parallel significant decrease in the expression of H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase was observed, as revealed by RT\u2014PCR, quantitative immunoblotting and immunofluorescence.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. In the present study, we demonstrate that the sustained inhibition of gastric secretion due to tumours producing PYY\/enteroglucagon in transgenic mice is associated with an increase in AQP4 expression and a down\u2010regulation of H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\u2010ATPase in parietal cells that acquire the characteristics of basal parietal cells. The absence of H<jats:sub>2<\/jats:sub> receptors\u2010mediated signalling due to the inhibition of histamine release from ECL (enterochromaffin\u2010like) cells by PYY may be in part responsible for the observed increase in the number of parietal cells expressing AQP4.<\/jats:p>","DOI":"10.1042\/bc20040138","type":"journal-article","created":{"date-parts":[[2005,8,24]],"date-time":"2005-08-24T12:55:20Z","timestamp":1124888120000},"page":"735-742","source":"Crossref","is-referenced-by-count":10,"title":["Altered expression of aquaporin 4 and H<sup>+<\/sup>\/K<sup>+<\/sup>\u2010ATPase in the stomachs of peptide YY (PYY) transgenic mice"],"prefix":"10.1111","volume":"97","author":[{"given":"M.","family":"Carmosino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Mazzone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Laforenza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Gastaldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Svelto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Valenti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,9]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"F601","article-title":"Immunolocalization of AE2 anion exchanger in rat kidney","volume":"273","author":"Alper S.L.","year":"1997","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(94)90094-9"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-4257(01)00037-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1989.tb25154.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200103010"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02091539"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00343955"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.9.2993"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-9335(98)80117-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00269161"},{"key":"e_1_2_7_12_1","first-page":"G161","article-title":"Functional heterogeneity of parietal cells along the pit\u2010gland axis","volume":"272","author":"Karam S.M.","year":"1997","journal-title":"Am. J. Physiol."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1469"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9441"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.LAB.0000048720.34096.D7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(86)90938-8"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.7.1.161"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00092-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.2.245"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1242\/dev.122.4.1157","article-title":"Peptide YY expression is an early event in colonic endocrine cell differentiation: evidence from normal and transgenic mice","volume":"122","author":"Upchurch B.H.","year":"1996","journal-title":"Development"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.40.24365"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/33.10.3900195"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.279.2.G448"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.84.7.5840"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02208679"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(01)00611-8"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70227-0"}],"container-title":["Biology of the Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FBC20040138","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/BC20040138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T19:59:13Z","timestamp":1696967953000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1042\/BC20040138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2005,9]]}},"alternative-id":["10.1042\/BC20040138"],"URL":"https:\/\/doi.org\/10.1042\/bc20040138","archive":["Portico"],"relation":{},"ISSN":["0248-4900","1768-322X"],"issn-type":[{"value":"0248-4900","type":"print"},{"value":"1768-322X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9]]}}}