{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:41:54Z","timestamp":1791175314680,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":1469,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>\n                    <jats:italic>Background information<\/jats:italic>\n                    . The IP\n                    <jats:sub>3<\/jats:sub>\n                    R (inositol 1,4,5\u2010trisphosphate receptor) is a tetrameric channel that accounts for a large part of the intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release in virtually all cell types. We have previously demonstrated that caspase\u20103\u2010mediated cleavage of IP\n                    <jats:sub>3<\/jats:sub>\n                    R1 during cell death generates a C\u2010terminal fragment of 95 kDa comprising the complete channel domain. Expression of this truncated IP\n                    <jats:sub>3<\/jats:sub>\n                    R increases the cellular sensitivity to apoptotic stimuli, and it was postulated to be a constitutively active channel.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    . In the present study, we demonstrate that expression of the caspase\u20103\u2010cleaved C\u2010terminus of IP\n                    <jats:sub>3<\/jats:sub>\n                    R1 increased the rate of thapsigargin\u2010mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    leak and decreased the rate of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake into the ER (endoplasmic reticulum), although it was not sufficient by itself to deplete intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    stores. We detected the truncated IP\n                    <jats:sub>3<\/jats:sub>\n                    R1 in different cell types after a challenge with apoptotic stimuli, as well as in aged mouse oocytes. Injection of mRNA corresponding to the truncated IP\n                    <jats:sub>3<\/jats:sub>\n                    R1 blocked sperm factor\u2010induced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations and induced an apoptotic phenotype.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . In the present study, we show that caspase\u20103\u2010mediated truncation of IP\n                    <jats:sub>3<\/jats:sub>\n                    R1 enhanced the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    leak from the ER. We suggest a model in which, in normal conditions, the increased Ca\n                    <jats:sup>2+<\/jats:sup>\n                    leak is largely compensated by enhanced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010uptake activity, whereas in situations where the cellular metabolism is compromised, as occurring in aging oocytes, the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    leak acts as a feed\u2010forward mechanism to divert the cell into apoptosis.\n                  <\/jats:p>","DOI":"10.1042\/bc20070086","type":"journal-article","created":{"date-parts":[[2007,12,19]],"date-time":"2007-12-19T10:28:52Z","timestamp":1198060132000},"page":"39-49","source":"Crossref","is-referenced-by-count":52,"title":["Caspase\u20103\u2010truncated type 1 inositol 1,4,5\u2010trisphosphate receptor enhances intracellular Ca\n                    <sup>2+<\/sup>\n                    leak and disturbs Ca\n                    <sup>2+<\/sup>\n                    signalling"],"prefix":"10.1111","volume":"100","author":[{"given":"Leen","family":"Verbert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bora","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Kocks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zerihun","family":"Assefa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan B.","family":"Parys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwig","family":"Missiaen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert","family":"Callewaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael A.","family":"Fissore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humbert","family":"De Smedt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geert","family":"Bultynck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,1,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403872200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/27094"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35036035"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.20.5450"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409650102"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3560345"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.17.9896"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200309146"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(03)00093-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207107"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","unstructured":"DevogelaereB.VerbertL.ParysJ.B.MissiaenL.DeSmedtH.The complex regulatory function of the ligand\u2010binding domain of the inositol 1 4 5\u2010trisphosphate receptorCell Calcium2007doi:10.1016\/j.ceca.2007.1004.1005","DOI":"10.1016\/j.ceca.2007.04.005"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1240745"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209990200"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402147"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.6.1828"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00616-8"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.48.34433"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.034926"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.6.3005"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-007-0719-7"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.06.029"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3180871"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00521-X"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/352241a0"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2004.04.007"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14700"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1124\/mol.105.013045","article-title":"Suramin and disulfonated stilbene derivatives stimulate the Ca2+\u2010induced Ca2+ release mechanism in A7r5 cells","volume":"68","author":"Kasri N. Nadif","year":"2005","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030599"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408352102"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1150"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(95)90070-5"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409657102"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401960"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)60155-4"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207105"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.04.008"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.11.3078"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(200003)55:3<299::AID-MRD8>3.0.CO;2-G"},{"key":"e_1_2_7_43_1","first-page":"21","article-title":"Proteolytic mechanisms leading to disturbed Ca2+ signalling in apoptotic cell death","volume":"2","author":"Verbert L.","year":"2007","journal-title":"Calcium Binding Proteins"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00561.2005"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1302"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.19.11678"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00601-9"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401661"}],"container-title":["Biology of the Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FBC20070086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/BC20070086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T17:39:13Z","timestamp":1697045953000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1042\/BC20070086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1042\/BC20070086"],"URL":"https:\/\/doi.org\/10.1042\/bc20070086","archive":["Portico"],"relation":{},"ISSN":["0248-4900","1768-322X"],"issn-type":[{"value":"0248-4900","type":"print"},{"value":"1768-322X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}