{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:15:00Z","timestamp":1791263700128,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":1287,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. TSPO (translocator protein), previously known as PBR (peripheral\u2010type benzodiazepine receptor), is a ubiquitous 18 kDa transmembrane protein that participates in diverse cell functions. High\u2010affinity TSPO ligands are best known for their ability to stimulate cholesterol transport in organs synthesizing steroids and bile salts, although they modulate other physiological functions, including cell proliferation, apoptosis and calcium\u2010dependent transepithelial ion secretion. In present study, we investigated the localization and function of TSPO in salivary glands.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. Immunohistochemical analysis of TSPO in rat salivary glands revealed that TSPO and its endogenous ligand, DBI (diazepam\u2010binding inhibitor), were present in duct and mucous acinar cells. TSPO was localized to the mitochondria of these cells, whereas DBI was cytosolic. As expected, mitochondrial membrane preparations, which were enriched in TSPO, exhibited a high affinity for the TSPO drug ligand,<jats:sup>3<\/jats:sup>H\u2010labelled PK 11195, as shown by<jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub>and<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>values of 10.0\u00b10.5 pmol\/mg and 4.0\u00b11.0 nM respectively. Intravenous perfusion of PK 11195 increased the salivary flow rate that was induced by muscarinic and \u03b1\u2010adrenergic agonists, whereas it had no effect when administered alone. Addition of PK 11195 also increased the K<jats:sup>+<\/jats:sup>, Na<jats:sup>+<\/jats:sup>, Cl<jats:sup>\u2212<\/jats:sup>and protein content of saliva, indicating that this ligand modulated secretion by acini and duct cells.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. High\u2010affinity ligand binding to mitochondrial TSPO modulates neurotransmitter\u2010induced salivary secretion by duct and mucous acinar cells of rat submandibular glands.<\/jats:p>","DOI":"10.1042\/bc20070157","type":"journal-article","created":{"date-parts":[[2008,5,8]],"date-time":"2008-05-08T10:15:11Z","timestamp":1210241711000},"page":"427-439","source":"Crossref","is-referenced-by-count":9,"title":["Effect of translocator protein (18 kDa)\u2010ligand binding on neurotransmitter\u2010induced salivary secretion in rat submandibular glands"],"prefix":"10.1111","volume":"100","author":[{"given":"Mariano A.","family":"Ostuni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar R.","family":"Tumilasci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"P\u00e9ranzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estela M. 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