{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:21:57Z","timestamp":1791296517596,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,1,3]],"date-time":"2012-01-03T00:00:00Z","timestamp":1325548800000},"content-version":"vor","delay-in-days":824,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. The TSPO (18 kDa translocator protein) is a mitochondrial transmembrane protein involved in cholesterol transport in organs that synthesize steroids and bile salts. Different natural and synthetic high\u2010affinity TSPO ligands have been characterized through their ability to stimulate cholesterol transport, but also to stimulate other physiological functions including cell proliferation, apoptosis and calcium\u2010dependent transepithelial ion secretion. Here, we investigate the localization and functions of TSPO in the small intestine.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. TSPO was present in enterocyte mitochondria but not in rat intestinal goblet cells. Enterocyte cytoplasm also contained the endogenous TSPO ligand, polypeptide DBI (diazepam\u2010binding inhibitor). Whereas intestinal TSPO had high affinity for the synthetic ligand PK 11195, the pharmacological profile of TSPO in the duodenum was distinct from the jejunum and ileum. Specifically, benzodiazepine Ro5\u20104864 and protoporphyrin IX showed 5\u201313\u2010fold lower affinity for duodenal TSPO. The mRNA and protein ratios of TSPO to other mitochondrial membrane proteins VDAC (voltage\u2010dependent anion channel) and ANT (adenine nucleotide transporter) were significantly different. PK 11195 stimulated calcium\u2010dependent chloride secretion in the duodenum and calcium\u2010dependent chloride absorption in the ileum, but did not affect jejunum ion transport.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. The functional differences in subpopulations of TSPO in different regions of the intestine could be related to structural organization of mitochondrial protein complexes that mediate the ability of TSPO to modulate either chloride secretion or absorption in the duodenum and ileum respectively.<\/jats:p>","DOI":"10.1042\/bc20080231","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T09:41:18Z","timestamp":1241689278000},"page":"573-586","source":"Crossref","is-referenced-by-count":23,"title":["Distribution, pharmacological characterization and function of the 18 kDa translocator protein in rat small intestine"],"prefix":"10.1111","volume":"101","author":[{"given":"Mariano Anibal","family":"Ostuni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"P\u00e9ranzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Ducroc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Fasseu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branislav","family":"Vidic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"Dumont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vassilios","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Jacques","family":"Lacapere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,1,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0022-3565(25)21237-X","article-title":"Peripheral\u2010type benzodiazepine receptors: autoradiographic localization in whole\u2010body sections of neonatal rats","volume":"233","author":"Anholt R.R.","year":"1985","journal-title":"J. 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