{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:40:24Z","timestamp":1698777624338},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,1,3]],"date-time":"2012-01-03T00:00:00Z","timestamp":1325548800000},"content-version":"vor","delay-in-days":520,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. Activation of MAPKs (mitogen\u2010activated protein kinases), in particular ERK1\/2 (extracellular\u2010signal\u2010regulated kinase 1\/2), has been reported to take place in a large variety of cell types after hypo\u2010osmotic cell swelling. Depending on cell type, ERK1\/2 phosphorylation can then serve or not the RVD (regulatory volume decrease) process. The present study investigates ERK1\/2 activation after aniso\u2010osmotic stimulations in turbot hepatocytes and the potential link between phosphorylation of these proteins and RVD.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. In turbot hepatocytes, Western\u2010blot analysis shows that a hypo\u2010osmotic shock from 320 to 240 mOsm\u00b7kg<jats:sup>\u22121<\/jats:sup> induced a rapid increase in ERK1\/2 phosphorylation, whereas a hyper\u2010osmotic shock from 320 to 400 mOsm\u00b7kg<jats:sup>\u22121<\/jats:sup> induced no significant change in the phosphorylation of these proteins. The hypo\u2010osmotic\u2010induced ERK1\/2 phosphorylation was significantly prevented when hypo\u2010osmotic shock was performed in the presence of the specific MEK (MAPK\/ERK kinase) inhibitor PD98059 (100 \u03bcM). In these conditions, the RVD process was not altered, suggesting that ERK1\/2 did not participate in this process in turbot hepatocytes. Moreover, the hypo\u2010osmotic\u2010induced activation of ERK1\/2 was significantly prevented by breakdown of extracellular ATP by apyrase (10 units\u00b7ml<jats:sup>\u22121<\/jats:sup>), by inhibition of purinergic P<jats:sub>2<\/jats:sub> receptors by suramin (100 \u03bcM) or by calcium depletion using EGTA (1 mM) and thapsigargin (1 \u03bcM).<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. In turbot hepatocytes, hypo\u2010osmotic swelling but not hyper\u2010osmotic shrinkage induced the activation of ERK1\/2. However, these proteins do not seem to be involved in the RVD process. Their hypo\u2010osmotic\u2010induced activation is partially due to cascades of signalling events triggered by the binding of released ATP on purinergic P<jats:sub>2<\/jats:sub> receptors and requires the presence of calcium.<\/jats:p>","DOI":"10.1042\/bc20090154","type":"journal-article","created":{"date-parts":[[2010,4,20]],"date-time":"2010-04-20T09:53:16Z","timestamp":1271757196000},"page":"447-456","source":"Crossref","is-referenced-by-count":4,"title":["Activation of the MAPKs ERK1\/2 by cell swelling in turbot hepatocytes"],"prefix":"10.1111","volume":"102","author":[{"given":"Audrey","family":"Fouchs","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Ollivier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Haond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stella","family":"Roy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Calv\u00e8s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Pichavant\u2010Rafini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.06.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-04-01196.1998"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00510.2002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2006.03.017"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.00117"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(00)80357-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/O09-009"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)62498-5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.28.16483"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2000.5866"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.3.C480"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021110300652"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1523\/JNEUROSCI.23-06-02348.2003","article-title":"Activation of extracellular signal\u2010regulated kinase by stretch\u2010induced injury in astrocytes involves extracellular ATP and P2 purinergic receptors","volume":"23","author":"Neary J.T.","year":"2003","journal-title":"J. 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