{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:23:20Z","timestamp":1789507400654,"version":"build-2803163510"},"reference-count":59,"publisher":"Portland Press Ltd.","issue":"11","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:p>Among the several mechanisms that contribute to MDR (multidrug resistance), the overexpression of drug-efflux pumps belonging to the ABC (ATP-binding cassette) superfamily is the most frequent cause of resistance to antifungal agents. The multidrug transporter proteins Cdr1p and Cdr2p of the ABCG subfamily are major players in the development of MDR in Candida albicans. Because several genes coding for ABC proteins exist in the genome of C. albicans, but only Cdr1p and Cdr2p have established roles in MDR, it is implicit that the other members of the ABC family also have alternative physiological roles. The present study focuses on an ABC transporter of C. albicans, Mlt1p, which is localized in the vacuolar membrane and specifically transports PC (phosphatidylcholine) into the vacuolar lumen. Transcriptional profiling of the mlt1\u2206\/\u2206 mutant revealed a down-regulation of the genes involved in endocytosis, oxidoreductase activity, virulence and hyphal development. High-throughput MS-based lipidome analysis revealed that the Mlt1p levels affect lipid homoeostasis and thus lead to a plethora of physiological perturbations. These include a delay in endocytosis, inefficient sequestering of reactive oxygen species (ROS), defects in hyphal development and attenuated virulence. The present study is an emerging example where new and unconventional roles of an ABC transporter are being identified.<\/jats:p>","DOI":"10.1042\/bcj20160024","type":"journal-article","created":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T01:44:07Z","timestamp":1459302247000},"page":"1537-1552","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Pleiotropic effects of the vacuolar ABC transporter <i>MLT1<\/i> of <i>Candida albicans<\/i> on cell function and virulence"],"prefix":"10.1042","volume":"473","author":[{"given":"Nitesh\u00a0Kumar","family":"Khandelwal","sequence":"first","affiliation":[{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Kaemmer","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute Jena (HKI), D-07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toni\u00a0M.","family":"F\u00f6rster","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute Jena (HKI), D-07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, 145 Life Sciences Building, Stony Brook, NY 11794, U.S.A."},{"name":"Department of Biochemistry, Lucknow University, Lucknow 226024, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alix\u00a0T.","family":"Coste","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, University of Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, CH-1011, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David\u00a0R.","family":"Andes","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of Wisconsin, Madison, WI 53792, U.S.A."},{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Hube","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knoell Institute Jena (HKI), D-07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Sanglard","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, University of Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, CH-1011, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeraj","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Public Health Research Institute, New Jersey Medical School, Rutgers, The State University of New Jersey, 225 Warren Street, Newark, NJ 07103, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupinder","family":"Kaur","sequence":"additional","affiliation":[{"name":"Laboratory of Fungal Pathogenesis, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500001, Andhra Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"d'Enfert","sequence":"additional","affiliation":[{"name":"D\u00e9partement G\u00e9nomes et G\u00e9n\u00e9tique, Institut Pasteur, Unit\u00e9 Biologie et Pathog\u00e9nicit\u00e9 Fongiques, Paris, France, INRA, USC2019, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok\u00a0Kumar","family":"Mondal","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra","family":"Prasad","sequence":"additional","affiliation":[{"name":"Amity Institute of Integrative Sciences and Health, Amity University Haryana, Amity Education Valley, Gurgaon 122413, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2016,5,27]]},"reference":[{"key":"2021112216285017200_B1","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.1128\/AAC.46.6.1704-1713.2002","article-title":"Resistance mechanisms in clinical isolates of Candida albicans","volume":"46","author":"White","year":"2002","journal-title":"Antimicrob. 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