{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:09:19Z","timestamp":1792591759618,"version":"4.5.0"},"reference-count":46,"publisher":"Portland Press Ltd.","issue":"21","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,11,1]]},"abstract":"<jats:p>The structurally related AB5-type heat-labile enterotoxins of Escherichia coli and Vibrio cholerae are classified into two major types. The type I group includes cholera toxin (CT) and E. coli LT-I, whereas the type II subfamily comprises LT-IIa, LT-IIb and LT-IIc. The carbohydrate-binding specificities of LT-IIa, LT-IIb and LT-IIc are distinctive from those of cholera toxin and E. coli LT-I. Whereas CT and LT-I bind primarily to the GM1 ganglioside, LT-IIa binds to gangliosides GD1a, GD1b and GM1, LT-IIb binds to the GD1a and GT1b gangliosides, and LT-IIc binds to GM1, GM2, GM3 and GD1a. These previous studies of the binding properties of type II B-subunits have been focused on ganglio core chain gangliosides. To further define the carbohydrate binding specificity of LT-IIb B-subunits, we have investigated its binding to a collection of gangliosides and non-acid glycosphingolipids with different core chains. A high-affinity binding of LT-IIb B-subunits to gangliosides with a neolacto core chain, such as Neu5Gc\u03b13- and Neu5Ac\u03b13-neolactohexaosylceramide, and Neu5Gc\u03b13- and Neu5Ac\u03b13-neolactooctaosylceramide was detected. An LT-IIb-binding ganglioside was isolated from human small intestine and characterized as Neu5Ac\u03b13-neolactohexaosylceramide. The crystal structure of the B-subunit of LT-IIb with the pentasaccharide moiety of Neu5Ac\u03b13-neolactotetraosylceramide (Neu5Ac-nLT: Neu5Ac\u03b13Gal\u03b24GlcNAc\u03b23Gal\u03b24Glc) was determined providing the first information for a sialic-binding site in this subfamily, with clear differences from that of CT and LT-I.<\/jats:p>","DOI":"10.1042\/bcj20160575","type":"journal-article","created":{"date-parts":[[2016,8,26]],"date-time":"2016-08-26T01:41:26Z","timestamp":1472175686000},"page":"3923-3936","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Biochemical and structural characterization of the novel sialic acid-binding site of <i>Escherichia coli<\/i> heat-labile enterotoxin LT-IIb"],"prefix":"10.1042","volume":"473","author":[{"given":"Dani","family":"Zalem","sequence":"first","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, G\u00f6teborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o P.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Centre de Recherches sur les Macromol\u00e9cules V\u00e9g\u00e9tales (UPR5301, CNRS and University Grenoble Alpes), BP53, F-38041 Grenoble Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabelle","family":"Varrot","sequence":"additional","affiliation":[{"name":"Centre de Recherches sur les Macromol\u00e9cules V\u00e9g\u00e9tales (UPR5301, CNRS and University Grenoble Alpes), BP53, F-38041 Grenoble Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lebens","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Microbiology and Immunology, The Sahlgrenska Academy, University of Gothenburg, G\u00f6teborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Imberty","sequence":"additional","affiliation":[{"name":"Centre de Recherches sur les Macromol\u00e9cules V\u00e9g\u00e9tales (UPR5301, CNRS and University Grenoble Alpes), BP53, F-38041 Grenoble Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susann","family":"Teneberg","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, The Sahlgrenska Academy, University of Gothenburg, G\u00f6teborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"key":"2021112216462646700_BCJ-2016-0575C1","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1128\/CMR.18.3.465-483.2005","article-title":"Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention","volume":"18","author":"Qadri","year":"2005","journal-title":"Clin. 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