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Knockdown of CaV1.3 or TRPV6 channels blocked osmotic stress and DSS-induced ROS production and attenuated TJ disruption and barrier dysfunction. N-Acetyl l-cysteine (NAC) and l-NG-Nitroarginine methyl ester (l-NAME) blocked stress-induced TJ disruption and barrier dysfunction. NAC and l-NAME also blocked stress-induced activation of c-Jun N-terminal kinase (JNK) and c-Src. ROS was colocalized with the mitochondrial marker in stressed cells. Cyclosporin A blocked osmotic stress and DSS-induced ROS production, barrier dysfunction, TJ disruption and JNK activation. Mitochondria-targeted Mito-TEMPO blocked osmotic stress and DSS-induced barrier dysfunction and TJ disruption. Chronic restraint stress in mice resulted in the elevation of intracellular Ca2+, activation of JNK and c-Src, and disruption of TJ in the colonic epithelium. Furthermore, corticosterone administration induced JNK and c-Src activation, TJ disruption and protein thiol oxidation in colonic mucosa. The present study demonstrates that oxidative stress is a common signal in the mechanism of TJ disruption in the intestinal epithelium by different types of cellular stress in vitro and bio behavioral stress in vivo.<\/jats:p>","DOI":"10.1042\/bcj20160679","type":"journal-article","created":{"date-parts":[[2017,1,6]],"date-time":"2017-01-06T02:00:20Z","timestamp":1483668020000},"page":"731-749","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Calcium-mediated oxidative stress: a common mechanism in tight junction disruption by different types of cellular stress"],"prefix":"10.1042","volume":"474","author":[{"given":"Ruchika","family":"Gangwar","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avtar S.","family":"Meena","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep K.","family":"Shukla","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana S.","family":"Nagaraja","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology and Center for RNA Interference and Non-Coding RNA, M.D. Anderson Cancer Center, Houston, TX, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr L.","family":"Dorniak","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology and Center for RNA Interference and Non-Coding RNA, M.D. Anderson Cancer Center, Houston, TX, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Pallikuth","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Waters","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Sood","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology and Center for RNA Interference and Non-Coding RNA, M.D. Anderson Cancer Center, Houston, TX, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RadhaKrishna","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Tennessee Health Science Center, Memphis, TN, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2017,2,20]]},"reference":[{"key":"2021112216513139400_BCJ-2016-0679C1","first-page":"591","article-title":"Stress and the gut: pathophysiology, clinical consequences, diagnostic approach and treatment options","volume":"62","author":"Konturek","year":"2011","journal-title":"J. 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