{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:20:42Z","timestamp":1792099242985,"version":"4.4.0"},"reference-count":76,"publisher":"Portland Press Ltd.","issue":"8","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,4,15]]},"abstract":"<jats:p>We studied the kinetics of extracellular ATP (ATPe) in Escherichia coli and their outer membrane vesicles (OMVs) stimulated with amphipatic peptides melittin (MEL) and mastoparan 7 (MST7). Real-time luminometry was used to measure ATPe kinetics, ATP release, and ATPase activity. The latter was also determined by following [32P]Pi released from [\u03b3-32P]ATP. E. coli was studied alone, co-incubated with Caco-2 cells, or in rat jejunum segments. In E. coli, the addition of [\u03b3-32P]ATP led to the uptake and subsequent hydrolysis of ATPe. Exposure to peptides caused an acute 3-fold (MST7) and 7-fold (MEL) increase in [ATPe]. In OMVs, ATPase activity increased linearly with [ATPe] (0.1\u20131\u2005\u00b5M). Exposure to MST7 and MEL enhanced ATP release by 3\u20137 fold, with similar kinetics to that of bacteria. In Caco-2 cells, the addition of ATP to the apical domain led to a steep [ATPe] increase to a maximum, with subsequent ATPase activity. The addition of bacterial suspensions led to a 6\u20137 fold increase in [ATPe], followed by an acute decrease. In perfused jejunum segments, exposure to E. coli increased luminal ATP 2 fold. ATPe regulation of E. coli depends on the balance between ATPase activity and ATP release. This balance can be altered by OMVs, which display their own capacity to regulate ATPe. E. coli can activate ATP release from Caco-2 cells and intestinal segments, a response which in vivo might lead to intestinal release of ATP from the gut lumen.<\/jats:p>","DOI":"10.1042\/bcj20160879","type":"journal-article","created":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T01:10:32Z","timestamp":1488330632000},"page":"1395-1416","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Dynamic regulation of extracellular ATP in<i>Escherichia coli<\/i>"],"prefix":"10.1042","volume":"474","author":[{"given":"Cora Lilia","family":"Alvarez","sequence":"first","affiliation":[{"name":"Universidad de Buenos Aires. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Qu\u00edmica y Fisico-Qu\u00edmica Biol\u00f3gicas (IQUIFIB) \u201cProf. Alejandro C. Paladini\u201d. Facultad de Farmacia y Bioqu\u00edmica. Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Corradi","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Qu\u00edmica y Fisico-Qu\u00edmica Biol\u00f3gicas (IQUIFIB) \u201cProf. Alejandro C. Paladini\u201d. Facultad de Farmacia y Bioqu\u00edmica. Buenos Aires, Argentina"},{"name":"Universidad de Buenos Aires. Facultad de Farmacia y Bioqu\u00edmica. Departamento de Qu\u00edmica Biol\u00f3gica. C\u00e1tedra de Qu\u00edmica Biol\u00f3gica Superior. Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Lauri","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Qu\u00edmica y Fisico-Qu\u00edmica Biol\u00f3gicas (IQUIFIB) \u201cProf. Alejandro C. Paladini\u201d. Facultad de Farmacia y Bioqu\u00edmica. Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Marginedas-Freixa","sequence":"additional","affiliation":[{"name":"UMR-S1134, Integrated Biology of Red Blood Cells, Inserm, Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Institut National de la Transfusion Sanguine, Laboratoire d'Excellence GR-Ex, F-75015. Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Florencia","family":"Leal Denis","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Qu\u00edmica y Fisico-Qu\u00edmica Biol\u00f3gicas (IQUIFIB) \u201cProf. Alejandro C. Paladini\u201d. Facultad de Farmacia y Bioqu\u00edmica. Buenos Aires, Argentina"},{"name":"Universidad de Buenos Aires. Facultad de Farmacia y Bioqu\u00edmica. Departamento de Qu\u00edmica Anal\u00edtica y Fisicoqu\u00edmica. C\u00e1tedra de Qu\u00edmica Anal\u00edtica. Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Enrique","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Plata. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP). Facultad de Ciencias Exactas. La Plata, Argentina"},{"name":"Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biol\u00f3gicas. C\u00e1tedra de Fisiolog\u00eda. La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina Mar\u00eda","family":"Mate","sequence":"additional","affiliation":[{"name":"Universidad Nacional de La Plata. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Investigaciones Bioqu\u00edmicas de La Plata (INIBIOLP) \"Prof. Dr. Rodolfo R. Brenner\". Facultad de Ciencias M\u00e9dicas. La Plata, Argentina"},{"name":"Universidad Nacional de La Plata. Facultad de Ciencias M\u00e9dicas. La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Milesi","sequence":"additional","affiliation":[{"name":"Universidad Nacional de la Plata. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP). Facultad de Ciencias Exactas. La Plata, Argentina"},{"name":"Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biol\u00f3gicas. C\u00e1tedra de Fisiolog\u00eda. La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano Anibal","family":"Ostuni","sequence":"additional","affiliation":[{"name":"UMR-S1134, Integrated Biology of Red Blood Cells, Inserm, Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Institut National de la Transfusion Sanguine, Laboratoire d'Excellence GR-Ex, F-75015. Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanesa","family":"Herlax","sequence":"additional","affiliation":[{"name":"Universidad Nacional de La Plata. Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas. Instituto de Investigaciones Bioqu\u00edmicas de La Plata (INIBIOLP) \"Prof. Dr. Rodolfo R. Brenner\". Facultad de Ciencias M\u00e9dicas. La Plata, Argentina"},{"name":"Universidad Nacional de La Plata. Facultad de Ciencias M\u00e9dicas. 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