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To identify potential interaction partners of the enzyme, quantitative mass spectrometry using stable isotope labeling of cells was combined with formaldehyde cross-linking and proximity biotinylation, respectively. Besides other enzymes involved in sphingolipid synthesis and intermembrane transfer of ceramide, and putative redox partners of FA2H, progesterone receptor membrane component-1 (PGRMC1) and PGRMC2 were identified as putative interaction partners. These two related heme-binding proteins are known to regulate several cytochrome P450 enzymes. Bimolecular fluorescence complementation experiments confirmed the interaction of FA2H with PGRMC1. Moreover, the PGRMC1 inhibitor AG-205 significantly reduced synthesis of hydroxylated ceramide and glucosylceramide in FA2H-expressing cells. This suggests that PGRMC1 may regulate FA2H activity, possibly through its heme chaperone activity.<\/jats:p>","DOI":"10.1042\/bcj20170963","type":"journal-article","created":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T16:10:09Z","timestamp":1518451809000},"page":"853-871","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification of progesterone receptor membrane component-1 as an interaction partner and possible regulator of fatty acid 2-hydroxylase"],"prefix":"10.1042","volume":"475","author":[{"given":"Robert","family":"Hardt","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, 53115 Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic","family":"Winter","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, 53115 Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volkmar","family":"Gieselmann","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, 53115 Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Eckhardt","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, 53115 Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,3,6]]},"reference":[{"key":"2021112217000971300_BCJ-2017-0963C1","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/j.bbalip.2009.12.004","article-title":"Fatty acid 2-Hydroxylation in mammalian sphingolipid biology","volume":"1801","author":"Hama","year":"2010","journal-title":"Biochim. 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