{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T18:02:43Z","timestamp":1783188163793,"version":"3.54.6"},"reference-count":64,"publisher":"Portland Press Ltd.","issue":"8","license":[{"start":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T00:00:00Z","timestamp":1585180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,4,30]]},"abstract":"<jats:p>We investigated acute effects of two allosteric protein kinase B (PKB) inhibitors, MK-2206 and Akti-1\/2, on insulin-stimulated lipogenesis in rat epididymal adipocytes incubated with fructose as carbohydrate substrate. In parallel, the phosphorylation state of lipogenic enzymes in adipocytes and incubated epididymal fat pads was monitored by immunoblotting. Preincubation of rat epididymal adipocytes with PKB inhibitors dose-dependently inhibited the following: insulin-stimulated lipogenesis, increased PKB Ser473 phosphorylation, increased PKB activity and decreased acetyl-CoA carboxylase (ACC) Ser79 phosphorylation. In contrast, the effect of insulin to decrease the phosphorylation of pyruvate dehydrogenase (PDH) at Ser293 and Ser300 was not abolished by PKB inhibition. Insulin treatment also induced ATP-citrate lyase (ACL) Ser454 phosphorylation, but this effect was less sensitive to PKB inhibitors than ACC dephosphorylation by insulin. In incubated rat epididymal fat pads, Akti-1\/2 treatment reversed insulin-induced ACC dephosphorylation, while ACL phosphorylation by insulin was maintained. ACL and ACC purified from white adipose tissue were poor substrates for PKB\u03b1 in vitro. However, effects of wortmannin and torin, along with Akti-1\/2 and MK-2206, on recognized PKB target phosphorylation by insulin were similar to their effects on insulin-induced ACL phosphorylation, suggesting that PKB could be the physiological kinase for ACL phosphorylation by insulin. In incubated epididymal fat pads from wild-type versus ACC1\/2 S79A\/S212A knockin mice, effects of insulin to increase lipogenesis from radioactive fructose or from radioactive acetate were reduced but not abolished. Together, the results support a key role for PKB in mediating insulin-stimulated lipogenesis by decreasing ACC phosphorylation, but not by decreasing PDH phosphorylation.<\/jats:p>","DOI":"10.1042\/bcj20190788","type":"journal-article","created":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T13:08:17Z","timestamp":1585228097000},"page":"1373-1389","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effects of PKB\/Akt inhibitors on insulin-stimulated lipogenesis and phosphorylation state of lipogenic enzymes in white adipose tissue"],"prefix":"10.1042","volume":"477","author":[{"given":"Nusrat","family":"Hussain","sequence":"first","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Ju","family":"Chuang","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Johanns","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Vertommen","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory R.","family":"Steinberg","sequence":"additional","affiliation":[{"name":"Department of Medicine, Centre for Metabolism, Obesity and Diabetes Research, McMaster University, Hamilton, Ontario, Canada"},{"name":"Department of Biochemistry and Biomedical Sciences, Centre for Metabolism, Obesity and Diabetes Research, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce E.","family":"Kemp","sequence":"additional","affiliation":[{"name":"St. Vincent's Institute of Medical Research, University of Melbourne, Fitzroy, Victoria, Australia"},{"name":"Department of Medicine, University of Melbourne, Fitzroy, Victoria, Australia"},{"name":"Mary MacKillop Institute for Health Research, Australian Catholic University, Fitzroy, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-8921","authenticated-orcid":false,"given":"Mark H.","family":"Rider","sequence":"additional","affiliation":[{"name":"Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"2021112218072893900_BCJ-2019-0788C1","first-page":"49","volume-title":"Metabolic Regulation","year":"1976"},{"key":"2021112218072893900_BCJ-2019-0788C2","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/0026-0495(71)90058-8","article-title":"Hormonal regulation of pyruvate dehydrogenase","volume":"20","year":"1971","journal-title":"Metabolism"},{"key":"2021112218072893900_BCJ-2019-0788C3","doi-asserted-by":"publisher","first-page":"38P","DOI":"10.1042\/bj1230038Pb","article-title":"Effects of insulin and Adrenaline on rat epididymal-fat-pad pyruvate dehydrogenase","volume":"123","year":"1971","journal-title":"Biochem. 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