{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:11:57Z","timestamp":1789499517718,"version":"build-2803163510"},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"5","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,3,13]]},"abstract":"<jats:p>Severe acute respiratory syndrome coronavirus is the causative agent of a respiratory disease with a high case fatality rate. During the formation of the coronaviral replication\/transcription complex, essential steps include processing of the conserved polyprotein nsp7\u201310 region by the main protease Mpro and subsequent complex formation of the released nsp's. Here, we analyzed processing of the coronavirus nsp7\u201310 region using native mass spectrometry showing consumption of substrate, rise and fall of intermediate products and complexation. Importantly, there is a clear order of cleavage efficiencies, which is influenced by the polyprotein tertiary structure. Furthermore, the predominant product is an nsp7+8(2\u2009:\u20092) hetero-tetramer with nsp8 scaffold. In conclusion, native MS, opposed to other methods, can expose the processing dynamics of viral polyproteins and the landscape of protein interactions in one set of experiments. Thereby, new insights into protein interactions, essential for generation of viral progeny, were provided, with relevance for development of antivirals.<\/jats:p>","DOI":"10.1042\/bcj20200029","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T08:09:09Z","timestamp":1582272549000},"page":"1009-1019","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":110,"title":["Processing of the SARS-CoV pp1a\/ab nsp7\u201310 region"],"prefix":"10.1042","volume":"477","author":[{"given":"Boris","family":"Krichel","sequence":"first","affiliation":[{"name":"Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistra\u00dfe 52, 20251 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Falke","sequence":"additional","affiliation":[{"name":"University of Hamburg, Institut f\u00fcr Biochemie und Molekularbiologie, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Hilgenfeld","sequence":"additional","affiliation":[{"name":"University of L\u00fcbeck, Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"},{"name":"German Center for Infection Research (DZIF), Hamburg-L\u00fcbeck-Borstel-Riems Site, University of L\u00fcbeck, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Redecke","sequence":"additional","affiliation":[{"name":"University of L\u00fcbeck, Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"},{"name":"Deutsches Elektronen Synchrotron (DESY), Notkestra\u00dfe 85, 22607 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1991-7922","authenticated-orcid":false,"given":"Charlotte","family":"Uetrecht","sequence":"additional","affiliation":[{"name":"Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistra\u00dfe 52, 20251 Hamburg, Germany"},{"name":"European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,3,13]]},"reference":[{"key":"2021112218064164600_BCJ-2020-0029C1","doi-asserted-by":"publisher","first-page":"1967","DOI":"10.1056\/NEJMoa030747","article-title":"Identification of a novel coronavirus in patients with severe acute respiratory syndrome","volume":"348","year":"2003","journal-title":"N. 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