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In the present study, we screened 155 chemicals from a microbial natural product library and found Geldanamycin, an HSP90 inhibitor, profoundly rescued THP-1 cells from pyroptosis induced by LPS plus Nigericin treatment. Consistently, other HSP90 inhibitors, including Radicicol, 17-DMAG and 17-AAG, all ameliorated pyroptosis in THP-1 cells by suppressing the inflammasome\/Caspase-1\/GSDMD signal pathway in pyroptosis. HSP90 inhibition compromised the protein stability of NLRP3, a critical component of the inflammasome. Moreover, up-regulated HSP70 may also contribute to this effect. HSP90 inhibition may thus be a potential therapeutic strategy in the treatment of inflammatory diseases in which pyroptosis plays a role.<\/jats:p>","DOI":"10.1042\/bcj20200351","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T00:51:22Z","timestamp":1591318282000},"page":"3923-3934","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Heat shock protein 90 inhibitors suppress pyroptosis in THP-1 cells"],"prefix":"10.1042","volume":"477","author":[{"given":"Zhou","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, School of Medicine, University of Missouri Kansas City, 2411 Holmes Street, Kansas City, MO 64108, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuzhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, School of Medicine, University of Missouri Kansas City, 2411 Holmes Street, Kansas City, MO 64108, U.S.A."},{"name":"Department of Microbiology, School of Medicine, Nanchang University, NO 461 Bayi Road, Donghu District, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yisong","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, School of Medicine, University of Missouri Kansas City, 2411 Holmes Street, Kansas City, MO 64108, U.S.A."},{"name":"The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, 1299 Xuefu Rd, Honggu District, Nanchang 330031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, School of Medicine, University of Missouri Kansas City, 2411 Holmes Street, Kansas City, MO 64108, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, Nanchang University, NO 461 Bayi Road, Donghu District, Nanchang 330006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6315-8372","authenticated-orcid":false,"given":"Mingui","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, School of Medicine, University of Missouri Kansas City, 2411 Holmes Street, Kansas City, MO 64108, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"2021112218101342400_BCJ-2020-0351C1","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1146\/annurev.immunol.021908.132620","article-title":"Immune and inflammatory mechanisms of atherosclerosis (*)","volume":"27","year":"2009","journal-title":"Ann. 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