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Previous work has indicated that the PARK16 locus, which harbors the gene encoding for Rab29, is involved in Parkinson's, and that Rab29 operates in a common pathway with LRRK2. Co-expression of Rab29 and LRRK2 stimulates LRRK2 activity by recruiting LRRK2 to the surface of the trans Golgi network. Here, we report that knock-out of Rab29 does not influence endogenous LRRK2 activity, based on the assessment of Rab10 and Rab12 phosphorylation, in wild-type LRRK2, LRRK2[R1441C] or VPS35[D620N] knock-in mouse tissues and primary cell lines, including brain extracts and embryonic fibroblasts. We find that in brain extracts, Rab12 phosphorylation is more robustly impacted by LRRK2 inhibitors and pathogenic mutations than Rab10 phosphorylation. Transgenic overexpression of Rab29 in a mouse model was also insufficient to stimulate basal LRRK2 activity. We observed that stimulation of Rab10 and Rab12 phosphorylation induced by agents that stress the endolysosomal system (nigericin, monensin, chloroquine and LLOMe) is suppressed by LRRK2 inhibitors but not blocked in Rab29 deficient cells. From the agents tested, nigericin induced the greatest increase in Rab10 and Rab12 phosphorylation (5 to 9-fold). Our findings indicate that basal, pathogenic, as well as nigericin and monensin stimulated LRRK2 pathway activity is not controlled by Rab29. Further work is required to establish how LRRK2 activity is regulated, and whether other Rab proteins can control LRRK2 by targeting it to diverse membranes.<\/jats:p>","DOI":"10.1042\/bcj20200458","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T12:21:03Z","timestamp":1604319663000},"page":"4397-4423","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity"],"prefix":"10.1042","volume":"477","author":[{"given":"Alexia F.","family":"Kalogeropulou","sequence":"first","affiliation":[{"name":"Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordana B.","family":"Freemantle","sequence":"additional","affiliation":[{"name":"Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel","family":"Lis","sequence":"additional","affiliation":[{"name":"Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmundo G.","family":"Vides","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Stanford University School of Medicine, Stanford 94305-5307, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole K.","family":"Polinski","sequence":"additional","affiliation":[{"name":"Michael J Fox Foundation for Parkinson's Research, Grand Central Station, PO Box 4777, New York, NY 10163, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2140-9185","authenticated-orcid":false,"given":"Dario R.","family":"Alessi","sequence":"additional","affiliation":[{"name":"Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"2021112218152571900_BCJ-2020-0458C1","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/j.neuron.2004.11.005","article-title":"Mutations in LRRK2 cause autosomal-dominant parkinsonism with pleomorphic pathology","volume":"44","year":"2004","journal-title":"Neuron"},{"key":"2021112218152571900_BCJ-2020-0458C2","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/j.neuron.2004.10.023","article-title":"Cloning of the gene containing mutations that cause PARK8-linked Parkinson's disease","volume":"44","year":"2004","journal-title":"Neuron"},{"key":"2021112218152571900_BCJ-2020-0458C3","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.ceb.2020.01.001","article-title":"Advances in elucidating the function of leucine-rich repeat protein kinase-2 in normal cells and Parkinson's disease","volume":"63","year":"2020","journal-title":"Curr. 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