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To gain further insight into its biological activity, we solved the structure of N-terminal histone chaperone domain of PfAsf1 (1\u2013159 amino acids) by X-ray crystallography to a resolution of 2.4\u2005\u00c5. The structure is composed of two beta-sheet to form a beta-sandwich, which resembles an immunoglobulin-like fold. The surface-charge distribution of PfAsf1 is distinct from yAsf1 and hAsf1 although the core-structure shows significant similarity. The crystal-structure indicated that PfAsf1 may exist in a dimeric-state which was further confirmed by solution cross-linking experiment. PfAsf1 was found to specifically interact with Plasmodium histone H3 and H4 and was able to deposit H3\/H4 dimer onto DNA-template to form disomes, showing its characteristic histone chaperone activity. We mapped the critical residues of PfAsf1 involved in histone H3\/H4 interaction and confirmed by site-directed mutagenesis. Further analysis indicates that histone interacting surface of Asf1 is highly conserved while the dimerization interface is variable. Our results identify the role of PfAsf1 as a mediator of chromatin assembly in Plasmodium falciparum, which is the causative agent of malignant malaria in humans.<\/jats:p>","DOI":"10.1042\/bcj20200891","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T11:26:36Z","timestamp":1611746796000},"page":"1117-1136","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Structural insights into histone chaperone Asf1 and its characterization from <i>Plasmodium falciparum<\/i>"],"prefix":"10.1042","volume":"478","author":[{"given":"Dushyant Kumar","family":"Srivastava","sequence":"first","affiliation":[{"name":"Structural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarika","family":"Gunjan","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrima","family":"Das","sequence":"additional","affiliation":[{"name":"Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India"},{"name":"Homi Bhaba National Institute, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1603-2969","authenticated-orcid":false,"given":"Vasudevan","family":"Seshadri","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8609-9803","authenticated-orcid":false,"given":"Siddhartha","family":"Roy","sequence":"additional","affiliation":[{"name":"Structural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,3,10]]},"reference":[{"key":"2021112218180801100_BCJ-2020-0891C1","volume-title":"World Malaria Report 2019","author":"WHO.","year":"2019"},{"key":"2021112218180801100_BCJ-2020-0891C2","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1038\/s41541-017-0015-7","article-title":"A malaria vaccine protects Aotus monkeys against virulent Plasmodium falciparum infection","volume":"2","year":"2017","journal-title":"npj Vaccines"},{"key":"2021112218180801100_BCJ-2020-0891C3","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1186\/s12864-020-6625-x","article-title":"Conserved associations between G-quadruplex-forming DNA motifs and virulence gene families in malaria parasites","volume":"21","year":"2020","journal-title":"BMC Genomics"},{"key":"2021112218180801100_BCJ-2020-0891C4","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1016\/j.pt.2020.07.004","article-title":"Artemisinin resistance and the unique selection pressure of a short-acting antimalarial","volume":"36","year":"2020","journal-title":"Trends Parasitol."},{"key":"2021112218180801100_BCJ-2020-0891C5","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1016\/S1473-3099(20)30271-1","article-title":"Knowing the enemy: genetics to track antimalarial resistance","volume":"20","year":"2020","journal-title":"Lancet Infect. 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