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This sequence contains the critical residues, which are conserved in the homolog of other Helicobacter gastric pathogens. However, the underlying basis for the role of this motif in catalytic function is not completely understood. Here, we used biochemical, biophysical and molecular dynamics simulations studies to determine that Glu155 of this stretch interacts with both Lys57 and Ser152. These interactions are essential for positioning of the motif through Trp159, which is located near Glu155 (His122\u2013Trp159\u2013Tyr125 contact is essential to tertiary structural integrity). The individual or double mutation of Lys57 and Ser152 to Ala considerably reduces catalytic activity with Lys57 to Ala being more significant, indicating they are crucial to function. Our data suggest that the Lys57\u2013Glu155\u2013Ser152 interaction influences the positioning of the loop containing the catalytic His133 so that this His can participate in catalysis, thereby providing a mechanistic understanding into the role of this motif in catalytic function. Lys57 was also found only in the arginases of other Helicobacter gastric pathogens. Based on the non-conserved motif, we found a new molecule, which specifically inhibits this enzyme. Thus, the present study not only provides a molecular basis into the role of this motif in function, but also offers an opportunity for the design of inhibitors with greater efficacy.<\/jats:p>","DOI":"10.1042\/bcj20200978","type":"journal-article","created":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T17:57:20Z","timestamp":1611424640000},"page":"871-894","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An evolutionary non-conserved motif in <i>Helicobacter pylori<\/i> arginase mediates positioning of the loop containing the catalytic residue for catalysis"],"prefix":"10.1042","volume":"478","author":[{"given":"Ankita","family":"Dutta","sequence":"first","affiliation":[{"name":"National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ditsa","family":"Sarkar","sequence":"additional","affiliation":[{"name":"National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Murarka","sequence":"additional","affiliation":[{"name":"National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tasneem","family":"Kausar","sequence":"additional","affiliation":[{"name":"National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satya","family":"Narayan","sequence":"additional","affiliation":[{"name":"Departments of Chemical Sciences, Tata Institute of Fundamental Research, Dr. Homi Bhabha Road, Colaba, Mumbai 400005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohit","family":"Mazumder","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sri Rama Koti","family":"Ainavarapu","sequence":"additional","affiliation":[{"name":"Departments of Chemical Sciences, Tata Institute of Fundamental Research, Dr. Homi Bhabha Road, Colaba, Mumbai 400005, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4090-3063","authenticated-orcid":false,"given":"Samudrala","family":"Gourinath","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5909-9442","authenticated-orcid":false,"given":"Apurba Kumar","family":"Sau","sequence":"additional","affiliation":[{"name":"National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,2,24]]},"reference":[{"key":"2021112218180236100_BCJ-2020-0978C1","doi-asserted-by":"publisher","first-page":"5","DOI":"10.3892\/ijo.2012.1701","article-title":"H. pylori infection and gastric cancer: State of the art (Review)","volume":"42","year":"2013","journal-title":"Int. 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