{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:37:33Z","timestamp":1791653853980,"version":"4.3.4"},"reference-count":77,"publisher":"Portland Press Ltd.","issue":"13","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,7,16]]},"abstract":"<jats:p>The coronavirus disease 2019 (COVID-19) pandemic, which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a global public health challenge. While the efficacy of vaccines against emerging and future virus variants remains unclear, there is a need for therapeutics. Repurposing existing drugs represents a promising and potentially rapid opportunity to find novel antivirals against SARS-CoV-2. The virus encodes at least nine enzymatic activities that are potential drug targets. Here, we have expressed, purified and developed enzymatic assays for SARS-CoV-2 nsp13 helicase, a viral replication protein that is essential for the coronavirus life cycle. We screened a custom chemical library of over 5000 previously characterized pharmaceuticals for nsp13 inhibitors using a fluorescence resonance energy transfer-based high-throughput screening approach. From this, we have identified FPA-124 and several suramin-related compounds as novel inhibitors of nsp13 helicase activity in vitro. We describe the efficacy of these drugs using assays we developed to monitor SARS-CoV-2 growth in Vero E6 cells.<\/jats:p>","DOI":"10.1042\/bcj20210201","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T19:15:43Z","timestamp":1625166943000},"page":"2405-2423","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp13 helicase"],"prefix":"10.1042","volume":"478","author":[{"given":"Jingkun","family":"Zeng","sequence":"first","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Weissmann","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agustina P.","family":"Bertolin","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"Posse","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berta","family":"Canal","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Ulferts","sequence":"additional","affiliation":[{"name":"Cell Biology of Infection Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Wu","sequence":"additional","affiliation":[{"name":"High Throughput Screening, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Harvey","sequence":"additional","affiliation":[{"name":"World Influenza Centre, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saira","family":"Hussain","sequence":"additional","affiliation":[{"name":"World Influenza Centre, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer C.","family":"Milligan","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe","family":"Roustan","sequence":"additional","affiliation":[{"name":"Structural Biology, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel","family":"Borg","sequence":"additional","affiliation":[{"name":"Structural Biology, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"McCoy","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, University College London, London WC1E 6BT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy S.","family":"Drury","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svend","family":"Kjaer","sequence":"additional","affiliation":[{"name":"Structural Biology, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"McCauley","sequence":"additional","affiliation":[{"name":"World Influenza Centre, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Howell","sequence":"additional","affiliation":[{"name":"High Throughput Screening, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupert","family":"Beale","sequence":"additional","affiliation":[{"name":"Cell Biology of Infection Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5184-7680","authenticated-orcid":false,"given":"John F.X.","family":"Diffley","sequence":"additional","affiliation":[{"name":"Chromosome Replication Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,7,2]]},"reference":[{"key":"2021112218233320600_BCJ-2021-0201C1","unstructured":"World-Health-Organization. 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