{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:44:44Z","timestamp":1784609084741,"version":"3.55.0"},"reference-count":50,"publisher":"Portland Press Ltd.","issue":"21","license":[{"start":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T00:00:00Z","timestamp":1634688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,11,12]]},"abstract":"<jats:p>The effects of small-molecule AMP-activated protein kinase (AMPK) activators in rat epididymal adipocytes were compared. SC4 was the most effective and submaximal doses of SC4 and 5-amino-4-imidazolecarboxamide (AICA) riboside were combined to study the effects of AMPK activation in white adipose tissue (WAT). Incubation of rat adipocytes with SC4\u2009+\u2009AICA riboside inhibited noradrenaline-induced lipolysis and decreased hormone-sensitive lipase (HSL) Ser563 phosphorylation, without affecting HSL Ser565 phosphorylation. Preincubation of fat pads from wild-type (WT) mice with SC4\u2009+\u2009AICA riboside inhibited insulin-stimulated lipogenesis from glucose or acetate and these effects were lost in AMPK\u03b11 knockout (KO) mice, indicating AMPK\u03b11 dependency. Moreover, in fat pads from acetyl-CoA carboxylase (ACC)1\/2 S79A\/S212A double knockin versus WT mice, the effect of SC4\u2009+\u2009AICA riboside to inhibit insulin-stimulated lipogenesis from acetate was lost, pinpointing ACC as the main AMPK target. Treatment with SC4\u2009+\u2009AICA riboside decreased insulin-stimulated glucose uptake, an effect that was still observed in fat pads from AMPK\u03b11 KO versus WT mice, suggesting the effect was partly AMPK\u03b11-independent. SC4\u2009+\u2009AICA riboside treatment had no effect on the insulin-induced increase in palmitate esterification nor on sn-glycerol-3-phosphate-O-acyltransferase activity. Therefore in WAT, AMPK activation inhibits noradrenaline-induced lipolysis and suppresses insulin-stimulated lipogenesis primarily by inactivating ACC and by inhibiting glucose uptake.<\/jats:p>","DOI":"10.1042\/bcj20210411","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T20:42:10Z","timestamp":1634762530000},"page":"3869-3889","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue"],"prefix":"10.1042","volume":"478","author":[{"given":"Sheng-Ju","family":"Chuang","sequence":"first","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Johanns","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Pyr dit Ruys","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory R.","family":"Steinberg","sequence":"additional","affiliation":[{"name":"Centre for Metabolism, Obesity and Diabetes Research, Department of Medicine and Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce E.","family":"Kemp","sequence":"additional","affiliation":[{"name":"St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, Fitzroy, Victoria, Australia"},{"name":"Mary MacKillop Institute for Health Research, Australian Catholic University, Fitzroy, VIC, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"Viollet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Institut Cochin, CNRS, INSERM, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-8921","authenticated-orcid":false,"given":"Mark H.","family":"Rider","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2021,11,9]]},"reference":[{"key":"2021112218261535700_BCJ-2021-0411C1","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1038\/nrm3311","article-title":"AMPK: a nutrient and energy sensor that maintains energy homeostasis","volume":"13","year":"2012","journal-title":"Nat. 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