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In the uncanonical N-terminal region of the parasite enzyme, we identified several autophosphorylation sites and probed their role in activity regulation of PfGSK3. By combining molecular modeling with experimental small-angle X-ray scattering data, we show that increased PfGSK3 activity is promoted by conformational changes in the PfGSK3 N-terminus, triggered by N-terminal phosphorylation. Our work provides novel insights into the structure and regulation of the malarial PfGSK3.<\/jats:p>","DOI":"10.1042\/bcj20210829","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T07:54:36Z","timestamp":1642060476000},"page":"337-356","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["N-terminal phosphorylation regulates the activity of glycogen synthase kinase 3 from\n                    <i>Plasmodium falciparum<\/i>"],"prefix":"10.1042","volume":"479","author":[{"given":"Samuel","family":"Pazicky","sequence":"first","affiliation":[{"name":"Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany"},{"name":"Molecular Biology Laboratory (EMBL), Hamburg Unit c\/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne","family":"Alder","sequence":"additional","affiliation":[{"name":"Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany"},{"name":"Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany"},{"name":"Department of Biology, University of Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haydyn","family":"Mertens","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory (EMBL), Hamburg Unit c\/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitri","family":"Svergun","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory (EMBL), Hamburg Unit c\/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Gilberger","sequence":"additional","affiliation":[{"name":"Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany"},{"name":"Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Germany"},{"name":"Department of Biology, University of Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0764-7483","authenticated-orcid":false,"given":"Christian","family":"L\u00f6w","sequence":"additional","affiliation":[{"name":"Centre for Structural Systems Biology (CSSB), Notkestrasse 85, D-22607 Hamburg, Germany"},{"name":"Molecular Biology Laboratory (EMBL), Hamburg Unit c\/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2022,2,4]]},"reference":[{"key":"2022020415382286800_BCJ-2021-0829C1","unstructured":"World Health Organization. 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