{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T13:28:19Z","timestamp":1743341299918,"version":"3.37.3"},"reference-count":62,"publisher":"Portland Press Ltd.","issue":"9","license":[{"start":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T00:00:00Z","timestamp":1649894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,5,13]]},"abstract":"<jats:p>Chromatin remodelling in spermatids is an essential step in spermiogenesis and involves the exchange of most histones by protamines, which drives chromatin condensation in late spermatids. The gene Rimklb encodes a citrylglutamate synthase highly expressed in testes of vertebrates and the increase of its reaction product, \u03b2-citrylglutamate, correlates in time with the appearance of spermatids. Here we show that deficiency in a functional Rimklb gene leads to male subfertility, which could be partially rescued by in vitro fertilization. Rimklb-deficient mice are impaired in a late step of spermiogenesis and produce spermatozoa with abnormally shaped heads and nuclei. Sperm chromatin in Rimklb-deficient mice was less condensed and showed impaired histone to protamine exchange and retained transition protein 2. These observations suggest that citrylglutamate synthase, probably via its reaction product \u03b2-citrylglutamate, is essential for efficient chromatin remodelling during spermiogenesis and may be a possible candidate gene for male subfertility or infertility in humans.<\/jats:p>","DOI":"10.1042\/bcj20210844","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T08:56:36Z","timestamp":1649926596000},"page":"953-972","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Citrylglutamate synthase deficient male mice are subfertile with impaired histone and transition protein 2 removal in late spermatids"],"prefix":"10.1042","volume":"479","author":[{"given":"Lihua","family":"Wang-Eckhardt","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Sylvester","sequence":"additional","affiliation":[{"name":"Core Facility Mass Spectrometry, Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivonne","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Pierre","family":"Allam","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, Medical Faculty, University of Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1964-9064","authenticated-orcid":false,"given":"Matthias","family":"Eckhardt","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2022,5,6]]},"reference":[{"key":"2022050613242891300_BCJ-2021-0844C1","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1007\/s00412-002-0227-z","article-title":"Roles of transition nuclear proteins in spermiogenesis","volume":"111","year":"2003","journal-title":"Chromosoma"},{"key":"2022050613242891300_BCJ-2021-0844C2","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.bbagrm.2013.08.004","article-title":"Chromatin dynamics during spermiogenesis","volume":"1839","year":"2014","journal-title":"Biochim. 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