{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:44:26Z","timestamp":1788187466784,"version":"build-2803163510"},"reference-count":46,"publisher":"Portland Press Ltd.","issue":"14","license":[{"start":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T00:00:00Z","timestamp":1688342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wellcome Trust","award":["201505\/Z\/16\/Z"],"award-info":[{"award-number":["201505\/Z\/16\/Z"]}]},{"name":"Chulabhorn Royal Academy"},{"name":"Wellcome Trust  Infection, Immunology, and Translational Medicine Doctoral Training Centre in Oxford."}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,7,26]]},"abstract":"<jats:p>Pseudomonas aeruginosa is a common cause of serious hospital-acquired infections, the leading proven cause of mortality in people with cystic fibrosis and is associated with high levels of antimicrobial resistance. Pyocins are narrow-spectrum protein antibiotics produced by P. aeruginosa that kill strains of the same species and have the potential to be developed as therapeutics targeting multi-drug resistant isolates. We have identified two novel pyocins designated SX1 and SX2. Pyocin SX1 is a metal-dependent DNase while pyocin SX2 kills cells through inhibition of protein synthesis. Mapping the uptake pathways of SX1 and SX2 shows these pyocins utilize a combination of the common polysaccharide antigen (CPA) and a previously uncharacterized TonB-dependent transporter (TBDT) PA0434 to traverse the outer membrane. In addition, TonB1 and FtsH are required by both pyocins to energize their transport into cells and catalyze their translocation across the inner membrane, respectively. Expression of PA0434 was found to be specifically regulated by copper availability and we have designated PA0434 as Copper Responsive Transporter A, or CrtA. To our knowledge these are the first S-type pyocins described that utilize a TBDT that is not involved in iron uptake.<\/jats:p>","DOI":"10.1042\/bcj20220552","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T16:07:46Z","timestamp":1688400466000},"page":"1035-1049","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Outer membrane translocation of pyocins via the copper regulated TonB-dependent transporter CrtA"],"prefix":"10.1042","volume":"480","author":[{"given":"Jiraphan","family":"Premsuriya","sequence":"first","affiliation":[{"name":"1School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, U.K."},{"name":"2Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok 10210, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khedidja","family":"Mosbahi","sequence":"additional","affiliation":[{"name":"1School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iva","family":"Atanaskovic","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Kleanthous","sequence":"additional","affiliation":[{"name":"3Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4206-2942","authenticated-orcid":false,"given":"Daniel","family":"Walker","sequence":"additional","affiliation":[{"name":"1School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, U.K."},{"name":"4Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2023,7,17]]},"reference":[{"key":"2023071713483148400_BCJ-2022-0552C1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","year":"2022","journal-title":"Lancet"},{"key":"2023071713483148400_BCJ-2022-0552C2","volume-title":"Prioritization of Pathogens to Guide Discovery, Research and Development of new Antibiotics for Drug Resistant Bacterial Infections, Including Tuberculosis","author":"WHO","year":"2017"},{"key":"2023071713483148400_BCJ-2022-0552C3","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1038\/s41579-021-00569-w","article-title":"The microbiome-shaping roles of bacteriocins","volume":"19","year":"2021","journal-title":"Nat. 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