{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:05:16Z","timestamp":1770897916779,"version":"3.50.1"},"reference-count":76,"publisher":"Portland Press Ltd.","issue":"3","license":[{"start":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:00:00Z","timestamp":1704758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["102172\/B\/13\/Z"],"award-info":[{"award-number":["102172\/B\/13\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/17\/56\/32925,FS\/EXT\/22\/35014,PG\/21\/10521"],"award-info":[{"award-number":["FS\/17\/56\/32925,FS\/EXT\/22\/35014,PG\/21\/10521"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/M009114\/1"],"award-info":[{"award-number":["MR\/M009114\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,2,7]]},"abstract":"<jats:p>Cysteine string protein \u03b1 (CSP\u03b1), also known as DNAJC5, is a member of the DnaJ\/Hsp40 family of co-chaperones. The name derives from a cysteine-rich domain, palmitoylation of which enables localisation to intracellular membranes, notably neuronal synaptic vesicles. Mutations in the DNAJC5 gene that encodes CSP\u03b1 cause autosomal dominant, adult-onset neuronal ceroid lipofuscinosis (ANCL), a rare neurodegenerative disease. As null mutations in CSP-encoding genes in flies, worms and mice similarly result in neurodegeneration, CSP is evidently an evolutionarily conserved neuroprotective protein. However, the client proteins that CSP chaperones to prevent neurodegeneration remain unclear. Traditional methods for identifying protein\u2013protein interactions, such as yeast 2-hybrid and affinity purification approaches, are poorly suited to CSP due to its requirement for membrane anchoring and its tendency to aggregate after cell lysis. Therefore, we employed proximity labelling, which enables the identification of interacting proteins in situ in living cells via biotinylation. Neuroendocrine PC12 cell lines stably expressing wild type or L115R ANCL mutant CSP constructs fused to miniTurbo were generated; then the biotinylated proteomes were analysed by liquid chromatography\u2013mass spectrometry and validated by western blotting. This confirmed several known CSP-interacting proteins, such as Hsc70 and SNAP-25, but also revealed novel binding proteins, including STXBP1\/Munc18-1. Interestingly, some protein interactions (such as Hsc70) were unaffected by the L115R mutation, whereas others (including SNAP-25 and STXBP1\/Munc18-1) were inhibited. These results define the CSP interactome in a neuronal model cell line and reveal interactions that are affected by ANCL mutation and hence may contribute to the neurodegeneration seen in patients.<\/jats:p>","DOI":"10.1042\/bcj20230319","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T09:59:52Z","timestamp":1704794392000},"page":"141-160","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Proximity labelling reveals effects of disease-causing mutation on the DNAJC5\/cysteine string protein \u03b1 interactome"],"prefix":"10.1042","volume":"481","author":[{"given":"Eleanor","family":"Barker","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy E.","family":"Milburn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nordine","family":"Helassa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dean E.","family":"Hammond","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Sanchez-Soriano","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0346-1289","authenticated-orcid":false,"given":"Alan","family":"Morgan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeff W.","family":"Barclay","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology, ISMIB, University of Liverpool, Liverpool, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"2024012913021498000_BCJ-2023-0319C1","doi-asserted-by":"publisher","first-page":"15","DOI":"10.3109\/01677069009084150","article-title":"A cysteine-string protein is expressed in retina and brain of Drosophila","volume":"7","year":"1990","journal-title":"J. Neurogenet."},{"key":"2024012913021498000_BCJ-2023-0319C2","doi-asserted-by":"publisher","first-page":"101758","DOI":"10.1016\/j.pneurobio.2020.101758","article-title":"Cysteine string proteins","volume":"188","year":"2020","journal-title":"Prog. Neurobiol."},{"key":"2024012913021498000_BCJ-2023-0319C3","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1126\/science.7906056","article-title":"Cysteine string proteins: a potential link between synaptic vesicles and presynaptic Ca2+\u2009channels","volume":"263","year":"1994","journal-title":"Science"},{"key":"2024012913021498000_BCJ-2023-0319C4","doi-asserted-by":"publisher","first-page":"19514","DOI":"10.1074\/jbc.271.32.19514","article-title":"Cysteine string proteins are associated with chromaffin granules","volume":"271","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C5","doi-asserted-by":"publisher","first-page":"5048","DOI":"10.1093\/emboj\/17.17.5048","article-title":"Cysteine string protein (CSP) is an insulin secretory granule-associated protein regulating beta-cell exocytosis","volume":"17","year":"1998","journal-title":"EMBO J."},{"key":"2024012913021498000_BCJ-2023-0319C6","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1242\/jcs.114.2.445","article-title":"The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4","volume":"114","year":"2001","journal-title":"J. Cell Sci."},{"key":"2024012913021498000_BCJ-2023-0319C7","doi-asserted-by":"publisher","first-page":"6332","DOI":"10.1038\/s41598-017-06710-1","article-title":"Primary fibroblasts from CSP\u03b1 mutation carriers recapitulate hallmarks of the adult onset neuronal ceroid lipofuscinosis","volume":"7","year":"2017","journal-title":"Sci. Rep."},{"key":"2024012913021498000_BCJ-2023-0319C8","doi-asserted-by":"publisher","first-page":"4024","DOI":"10.1038\/s41467-021-24337-9","article-title":"The bacterial toxin ExoU requires a host trafficking chaperone for transportation and to induce necrosis","volume":"12","year":"2021","journal-title":"Nat. Commun."},{"issue":"Pt 2","key":"2024012913021498000_BCJ-2023-0319C9","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1042\/bj3350205","article-title":"The cysteine-string domain of the secretory vesicle cysteine-string protein is required for membrane targeting","volume":"335","year":"1998","journal-title":"Biochem. J."},{"key":"2024012913021498000_BCJ-2023-0319C10","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/0014-5793(96)00026-9","article-title":"Association of Drosophila cysteine string proteins with membranes","volume":"380","year":"1996","journal-title":"FEBS Lett."},{"key":"2024012913021498000_BCJ-2023-0319C11","doi-asserted-by":"publisher","first-page":"4748","DOI":"10.1091\/mbc.e06-03-0183","article-title":"Dual role of the cysteine-string domain in membrane binding and palmitoylation-dependent sorting of the molecular chaperone cysteine-string protein","volume":"17","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"2024012913021498000_BCJ-2023-0319C12","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1126\/science.8310297","article-title":"Paralysis and early death in cysteine string protein mutants of Drosophila","volume":"263","year":"1994","journal-title":"Science"},{"key":"2024012913021498000_BCJ-2023-0319C13","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/0896-6273(94)90255-0","article-title":"Presynaptic dysfunction in Drosophila csp mutants","volume":"13","year":"1994","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C14","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1523\/JNEUROSCI.20-04-01281.2000","article-title":"Comparison of cysteine string protein (Csp) and mutant \u03b1-SNAP overexpression reveals a role for Csp in late steps of membrane fusion in dense-core granule exocytosis in adrenal chromaffin cells","volume":"20","year":"2000","journal-title":"J. Neurosci."},{"key":"2024012913021498000_BCJ-2023-0319C15","doi-asserted-by":"publisher","first-page":"47877","DOI":"10.1074\/jbc.M108186200","article-title":"Phosphorylation of cysteine string protein by protein kinase A. Implications for the modulation of exocytosis","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C16","doi-asserted-by":"publisher","first-page":"16212","DOI":"10.1021\/bi048612+","article-title":"The variable C-terminus of cysteine string proteins modulates exocytosis and protein-protein interactions","volume":"43","year":"2004","journal-title":"Biochemistry"},{"key":"2024012913021498000_BCJ-2023-0319C17","doi-asserted-by":"publisher","first-page":"2259","DOI":"10.1091\/mbc.9.8.2259","article-title":"Cysteine string protein functions directly in regulated exocytosis","volume":"9","year":"1998","journal-title":"Mol. Biol. Cell"},{"key":"2024012913021498000_BCJ-2023-0319C18","doi-asserted-by":"publisher","first-page":"e99180","DOI":"10.1371\/journal.pone.0099180","article-title":"Phosphomimetic mutation of cysteine string protein-\u03b1 increases the rate of regulated exocytosis by modulating fusion pore dynamics in PC12 cells","volume":"9","year":"2014","journal-title":"PLoS One"},{"key":"2024012913021498000_BCJ-2023-0319C19","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/S0896-6273(04)00190-4","article-title":"The synaptic vesicle protein CSP\u03b1 prevents presynaptic degeneration","volume":"42","year":"2004","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C20","doi-asserted-by":"publisher","first-page":"5916","DOI":"10.1093\/hmg\/ddu316","article-title":"Caenorhabditis elegans dnj-14, the orthologue of the DNAJC5 gene mutated in adult onset neuronal ceroid lipofuscinosis, provides a new platform for neuroprotective drug screening and identifies a SIR-2.1-independent action of resveratrol","volume":"23","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"2024012913021498000_BCJ-2023-0319C21","doi-asserted-by":"publisher","first-page":"165614","DOI":"10.1016\/j.bbadis.2019.165614","article-title":"The contribution of multicellular model organisms to neuronal ceroid lipofuscinosis research","volume":"1866","year":"2020","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"2024012913021498000_BCJ-2023-0319C22","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.ajhg.2011.07.003","article-title":"Mutations in DNAJC5, encoding cysteine-string protein alpha, cause autosomal-dominant adult-onset neuronal ceroid lipofuscinosis","volume":"89","year":"2011","journal-title":"Am. J. Hum. Genet."},{"key":"2024012913021498000_BCJ-2023-0319C23","doi-asserted-by":"publisher","first-page":"e26741","DOI":"10.1371\/journal.pone.0026741","article-title":"Exome-sequencing confirms DNAJC5 mutations as cause of adult neuronal ceroid-lipofuscinosis","volume":"6","year":"2011","journal-title":"PLoS One"},{"key":"2024012913021498000_BCJ-2023-0319C24","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1111\/cge.12020","article-title":"Recurrent mutations in DNAJC5 cause autosomal dominant Kufs disease","volume":"83","year":"2013","journal-title":"Clin. Genet."},{"key":"2024012913021498000_BCJ-2023-0319C25","doi-asserted-by":"publisher","first-page":"e29729","DOI":"10.1371\/journal.pone.0029729","article-title":"Mutations in the gene DNAJC5 cause autosomal dominant Kufs disease in a proportion of cases: study of the Parry family and 8 other families","volume":"7","year":"2012","journal-title":"PLoS One"},{"key":"2024012913021498000_BCJ-2023-0319C26","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1038\/s41431-019-0567-2","article-title":"Autosomal-dominant adult neuronal ceroid lipofuscinosis caused by duplication in DNAJC5 initially missed by Sanger and whole-exome sequencing","volume":"28","year":"2020","journal-title":"Eur. J. Hum. Genet."},{"key":"2024012913021498000_BCJ-2023-0319C27","doi-asserted-by":"publisher","first-page":"829573","DOI":"10.3389\/fnagi.2022.829573","article-title":"Adult-onset neuronal ceroid lipofuscinosis with a novel DNAJC5 mutation exhibits aberrant protein palmitoylation","volume":"14","year":"2022","journal-title":"Front. Aging Neurosci."},{"key":"2024012913021498000_BCJ-2023-0319C28","doi-asserted-by":"publisher","first-page":"2136","DOI":"10.1016\/j.bbadis.2014.07.009","article-title":"Oligomerization of cysteine string protein alpha mutants causing adult neuronal ceroid lipofuscinosis","volume":"1842","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"2024012913021498000_BCJ-2023-0319C29","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1038\/s41594-020-0375-y","article-title":"Aggregation of mutant cysteine string protein-alpha via Fe-S cluster binding is mitigated by iron chelators","volume":"27","year":"2020","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2024012913021498000_BCJ-2023-0319C30","doi-asserted-by":"publisher","first-page":"37330","DOI":"10.1074\/jbc.M112.389098","article-title":"Palmitoylation-induced aggregation of cysteine-string protein mutants that cause neuronal ceroid lipofuscinosis","volume":"287","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C31","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1038\/s41598-017-00036-8","article-title":"A cluster of palmitoylated cysteines are essential for aggregation of cysteine-string protein mutants that cause neuronal ceroid lipofuscinosis","volume":"7","year":"2017","journal-title":"Sci. Rep."},{"key":"2024012913021498000_BCJ-2023-0319C32","doi-asserted-by":"publisher","first-page":"25989","DOI":"10.1074\/jbc.271.42.25989","article-title":"The cysteine string secretory vesicle protein activates Hsc70 ATPase","volume":"271","year":"1996","journal-title":"J. Biol. Chem."},{"issue":"Pt 3","key":"2024012913021498000_BCJ-2023-0319C33","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1042\/bj3220853","article-title":"Activation of the ATPase activity of heat-shock proteins Hsc70\/Hsp70 by cysteine-string protein","volume":"322","year":"1997","journal-title":"Biochem. J."},{"key":"2024012913021498000_BCJ-2023-0319C34","doi-asserted-by":"publisher","first-page":"1781","DOI":"10.1046\/j.1471-4159.2000.0741781.x","article-title":"Cysteine-string protein: the chaperone at the synapse","volume":"74","year":"2000","journal-title":"J. Neurochem."},{"key":"2024012913021498000_BCJ-2023-0319C35","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1038\/ncb2131","article-title":"CSP\u03b1 promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity","volume":"13","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"2024012913021498000_BCJ-2023-0319C36","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1038\/emboj.2011.467","article-title":"CSP\u03b1 knockout causes neurodegeneration by impairing SNAP-25 function","volume":"31","year":"2012","journal-title":"EMBO J."},{"key":"2024012913021498000_BCJ-2023-0319C37","doi-asserted-by":"publisher","first-page":"13488","DOI":"10.1074\/jbc.273.22.13488","article-title":"Interaction of cysteine string proteins with the \u03b11A subunit of the P\/Q-type calcium channel","volume":"273","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C38","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1016\/S0896-6273(00)80811-9","article-title":"Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo","volume":"23","year":"1999","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C39","doi-asserted-by":"publisher","first-page":"10270","DOI":"10.1523\/JNEUROSCI.19-23-10270.1999","article-title":"Overexpression of cysteine-string proteins in Drosophila reveals interactions with syntaxin","volume":"19","year":"1999","journal-title":"J. Neurosci."},{"key":"2024012913021498000_BCJ-2023-0319C40","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1042\/bj20020123","article-title":"Phosphorylation-dependent interaction of the synaptic vesicle proteins cysteine string protein and synaptotagmin I","volume":"364","year":"2002","journal-title":"Biochem. J."},{"key":"2024012913021498000_BCJ-2023-0319C41","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1096\/fj.09-152033","article-title":"A charged prominence in the linker domain of the cysteine-string protein CSP\u03b1 mediates its regulated interaction with the calcium sensor synaptotagmin 9 during exocytosis","volume":"25","year":"2011","journal-title":"FASEB J."},{"key":"2024012913021498000_BCJ-2023-0319C42","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.neuron.2012.01.029","article-title":"Identification of CSP\u03b1 clients reveals a role in dynamin 1 regulation","volume":"74","year":"2012","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C43","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/S0896-6273(00)00096-9","article-title":"Cysteine string protein regulates G protein modulation of N-type calcium channels","volume":"28","year":"2000","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C44","doi-asserted-by":"publisher","first-page":"30236","DOI":"10.1074\/jbc.M500722200","article-title":"Characterization of the G\u03b1s regulator cysteine string protein","volume":"280","year":"2005","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C45","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/j.bbrc.2008.10.069","article-title":"Phosphorylation of cysteine string protein on Serine 10 triggers 14-3-3 protein binding","volume":"377","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2024012913021498000_BCJ-2023-0319C46","doi-asserted-by":"publisher","first-page":"2967","DOI":"10.1242\/jcs.00595","article-title":"Molecular determinants of cysteine string protein modulation of N-type calcium channels","volume":"116","year":"2003","journal-title":"J. Cell Sci."},{"key":"2024012913021498000_BCJ-2023-0319C47","doi-asserted-by":"publisher","first-page":"2447","DOI":"10.1038\/srep02447","article-title":"The large conductance, calcium-activated K+ (BK) channel is regulated by cysteine string protein","volume":"3","year":"2013","journal-title":"Sci. Rep."},{"key":"2024012913021498000_BCJ-2023-0319C48","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1038\/s41421-018-0012-7","article-title":"DNAJC5 facilitates USP19-dependent unconventional secretion of misfolded cytosolic proteins","volume":"4","year":"2018","journal-title":"Cell Discov."},{"key":"2024012913021498000_BCJ-2023-0319C49","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.15252\/embj.201593489","article-title":"DnaJ\/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins","volume":"35","year":"2016","journal-title":"EMBO J."},{"key":"2024012913021498000_BCJ-2023-0319C50","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1080\/15548627.2022.2065618","article-title":"Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5\/CSP\u03b1 mutants causes lipofuscin accumulation","volume":"19","year":"2022","journal-title":"Autophagy"},{"key":"2024012913021498000_BCJ-2023-0319C51","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1038\/s41598-017-01115-6","article-title":"Neurons export extracellular vesicles enriched in cysteine string protein and misfolded protein cargo","volume":"7","year":"2017","journal-title":"Sci. Rep."},{"key":"2024012913021498000_BCJ-2023-0319C52","doi-asserted-by":"publisher","first-page":"14359","DOI":"10.1074\/jbc.RA118.003660","article-title":"Secretion of misfolded cytosolic proteins from mammalian cells is independent of chaperone-mediated autophagy","volume":"293","year":"2018","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C53","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1016\/0028-3908(95)00114-L","article-title":"Cysteine string protein, a DnaJ family member, is present on diverse secretory vesicles","volume":"34","year":"1995","journal-title":"Neuropharmacology"},{"key":"2024012913021498000_BCJ-2023-0319C54","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1016\/S0006-291X(02)02964-9","article-title":"Oligomerization characteristics of cysteine string protein","volume":"300","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2024012913021498000_BCJ-2023-0319C55","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/S0171-9335(99)80079-X","article-title":"Two distinct domains in hsc70 are essential for the interaction with the synaptic vesicle cysteine string protein","volume":"78","year":"1999","journal-title":"Eur. J. Cell Biol."},{"key":"2024012913021498000_BCJ-2023-0319C56","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1016\/S0896-6273(01)00427-5","article-title":"A trimeric protein complex functions as a synaptic chaperone machine","volume":"31","year":"2001","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C57","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1038\/nbt.4201","article-title":"Efficient proximity labeling in living cells and organisms with TurboID","volume":"36","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"2024012913021498000_BCJ-2023-0319C58","doi-asserted-by":"publisher","first-page":"E2453","DOI":"10.1073\/pnas.1315147111","article-title":"Probing nuclear pore complex architecture with proximity-dependent biotinylation","volume":"111","year":"2014","journal-title":"Proc. Natl Acad. Sci. U.S.A."},{"key":"2024012913021498000_BCJ-2023-0319C59","doi-asserted-by":"publisher","first-page":"25014","DOI":"10.1074\/jbc.M802140200","article-title":"Palmitoylation and membrane interactions of the neuroprotective chaperone cysteine-string protein","volume":"283","year":"2008","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C60","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1523\/JNEUROSCI.1649-17.2017","article-title":"The role of cysteine string protein \u03b1 phosphorylation at Serine 10 and 34 by protein kinase C\u03b3 for presynaptic maintenance","volume":"38","year":"2018","journal-title":"J. Neurosci."},{"key":"2024012913021498000_BCJ-2023-0319C61","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1016\/j.str.2016.06.009","article-title":"Phosphorylation of cysteine string protein triggers a major conformational switch","volume":"24","year":"2016","journal-title":"Structure"},{"key":"2024012913021498000_BCJ-2023-0319C62","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1111\/cge.13829","article-title":"Autosomal dominant neuronal ceroid lipofuscinosis: clinical features and molecular basis","volume":"99","year":"2021","journal-title":"Clin. Genet."},{"key":"2024012913021498000_BCJ-2023-0319C63","doi-asserted-by":"publisher","first-page":"2549","DOI":"10.1016\/j.molcel.2021.04.012","article-title":"Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains","volume":"81","year":"2021","journal-title":"Mol. Cell"},{"key":"2024012913021498000_BCJ-2023-0319C64","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/s13024-017-0149-0","article-title":"The SH-SY5Y cell line in Parkinson's disease research: a systematic review","volume":"12","year":"2017","journal-title":"Mol. Neurodegener."},{"key":"2024012913021498000_BCJ-2023-0319C65","doi-asserted-by":"publisher","first-page":"Unit 15.12","DOI":"10.1002\/0471143030.cb1512s36","article-title":"Analysis of regulated secretion using PC12 cells","volume":"Chapter 15","year":"2007","journal-title":"Curr. Protoc. Cell Biol."},{"key":"2024012913021498000_BCJ-2023-0319C66","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","year":"2009","journal-title":"Nat. Protoc."},{"key":"2024012913021498000_BCJ-2023-0319C67","doi-asserted-by":"publisher","first-page":"19.23.11","DOI":"10.1002\/cpps.51","article-title":"BioID: a screen for protein-protein interactions","volume":"91","year":"2018","journal-title":"Curr. Protoc. Protein Sci."},{"key":"2024012913021498000_BCJ-2023-0319C68","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.cell.2005.09.028","article-title":"\u03b1-Synuclein cooperates with CSP\u03b1 in preventing neurodegeneration","volume":"123","year":"2005","journal-title":"Cell"},{"key":"2024012913021498000_BCJ-2023-0319C69","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1038\/nrm2002","article-title":"SNAREs\u2013engines for membrane fusion","volume":"7","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2024012913021498000_BCJ-2023-0319C70","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1126\/science.287.5454.864","article-title":"Synaptic assembly of the brain in the absence of neurotransmitter secretion","volume":"287","year":"2000","journal-title":"Science"},{"key":"2024012913021498000_BCJ-2023-0319C71","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.neuron.2012.02.019","article-title":"Motorneurons require cysteine string protein-\u03b1 to maintain the readily releasable vesicular pool and synaptic vesicle recycling","volume":"74","year":"2012","journal-title":"Neuron"},{"key":"2024012913021498000_BCJ-2023-0319C72","doi-asserted-by":"publisher","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","article-title":"Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ","volume":"13","year":"2014","journal-title":"Mol. Cell. Proteomics"},{"key":"2024012913021498000_BCJ-2023-0319C73","doi-asserted-by":"publisher","first-page":"1974","DOI":"10.1080\/15548627.2020.1712109","article-title":"Cytosolic domain of SIDT2 carries an arginine-rich motif that binds to RNA\/DNA and is important for the direct transport of nucleic acids into lysosomes","volume":"16","year":"2020","journal-title":"Autophagy"},{"key":"2024012913021498000_BCJ-2023-0319C74","doi-asserted-by":"publisher","first-page":"53072","DOI":"10.1074\/jbc.M306230200","article-title":"Cysteine string protein (CSP) inhibition of N-type calcium channels is blocked by mutant huntingtin","volume":"278","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2024012913021498000_BCJ-2023-0319C75","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1038\/nprot.2016.018","article-title":"Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2","volume":"11","year":"2016","journal-title":"Nat. Protoc."},{"key":"2024012913021498000_BCJ-2023-0319C76","doi-asserted-by":"publisher","first-page":"D442","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE database and related tools and resources in 2019: improving support for quantification data","volume":"47","year":"2019","journal-title":"Nucleic Acids Res."}],"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/481\/3\/141\/953693\/bcj-2023-0319.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/481\/3\/141\/953693\/bcj-2023-0319.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T13:02:43Z","timestamp":1706533363000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/481\/3\/141\/233957\/Proximity-labelling-reveals-effects-of-disease"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":76,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,2,7]]}},"URL":"https:\/\/doi.org\/10.1042\/bcj20230319","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,29]]}}}